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81.
LUAN Yongjiao XIE Zhixun WANG Sheng LUO Sisi ZHANG Lei XIE Liji XIE Zhiqin DENG Xianwen ZHANG Minxiu ZHANG Yanfang ZENG Tingting FAN Qing 《中国畜牧兽医》2007,47(12):3793-3804
To understand the genetic characteristics of fowl adenovirus serotype 4 (FAdV-4) in Guangxi region,the whole-genome sequencing were performed on a FAdV-4 strain GX2019-010 isolated from Guangxi in this study.Genome-wide nucleotide sequences were analyzed for homology alignment and genetic evolution,and the amino acid sequences of the major structural proteins Hexon,Penton and Fiber were further analyzed.The results showed that GX2019-010 had a full length of 43 719 bp and GC content of 54.9%,mainly including 43 potential protein coding regions.Genome-wide nucleotide homology comparisons revealed that GX2019-010 was 95.8% homologous to the pathogenic strain MX-SHP95,and 95.0% and 95.2% homologous to non-pathogenic strains ON1 and KR5,respectively.Genetic evolutionary analysis showed that GX2019-010 was in the same branch as the popular strains of FAdV-4 in China in recent years,but not in the same branch as the foreign strains of FAdV-4.This results indicated that there were genetic differences in FAdV-4.The main differences was at the right end of the genome and all domestic strains isolated in China were 1 966 bp missing including ORF19,ORF48 and ORF27 genes.It showed that FAdV-4 popular in China was territorial.Analysis of the amino acid sequences of the major structural proteins of Hexon,Penton and Fiber revealed that all of the three major structural proteins had mutations in amino acid sites,of which Fiber-2 had the most mutations.This study enriched the gene pool of FAdV-4 in Guangxi region and laid the foundation for future research on the prevention and control of HHS and epidemiological investigation. 相似文献
82.
从患眼炎、鼻窦炎的不同地点、不同时间、不同发病鸡群的病料共分离到17株细菌,经形态学、培养特性、生化试验和动物回归试验等结果表明,该细菌能运动、无芽孢的革兰氏阴性小杆菌,即禽波氏杆菌。该菌在鲜血平板上产生β溶血,在麦康凯平板上生长良好,形成光滑中央突起的珍珠状菌落。不利用糖类,不能在6.5%NaCl培养基中生长,尿素酶和硝酸盐还原试验均呈阳性,能凝集豚鼠和其他动物红血球。人工接种细菌均能使雏鸡发生相似于自然病例的临床症状和死亡,并且从病变部位回收到相应细菌。 相似文献
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Shinjiro OJIMA Masashi OKAMURA Nana OSAWA Akiko TAMURA Kazuki YOSHIOKA Takashige KASHIMOTO Takeshi HANEDA Hisaya K. ONO Dong-Liang HU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1147
Salmonella enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) is a host-specific pathogen causing systemic infection in poultry, which leads to significant economic losses due to high mortality. However, little is known about the dynamic process of systemic infection and pathogenic characteristics of S. Gallinarum in chickens. In the present study, we developed an oral infection model that reproduces the pathology of S. Gallinarum and clarified the host immune response of the infected chickens. Chickens at 20 days of age orally inoculated at a dose of 108 colony forming unit (CFU) showed typical clinical signs of fowl typhoid and died between 6 and 10 days post infection. The inoculated S. Gallinarum rapidly disseminated to multple organs and the bacterial counts increased in the liver and spleen at 3 days post infection. Pathological changes associated wirh inflammation in the liver and spleen became apparent at 4 days post infection, and increased expression of interferon (IFN)-γ and interleuikin (IL)-12 in the liver and spleen did not observed until 3 days post infection. These results indicate that S. Gallinarum rapidly spread to entire body through intestine, and the low-level of inflammatory responses in the liver during the early stage of infection may contribute to rapid, systemic dissemination of the bacteria. Our infection model and findings will contribute to the better understanding of the pathogenic mechanism of S. Gallinarum, and provide new insights into the prevention and control of fowl typhoid. 相似文献
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氧氟沙星在乌骨鸡体内残留消除规律研究 总被引:1,自引:0,他引:1
采用高效液相色谱法分析氧氟沙星在乌骨鸡体内各组织中的残留消除规律。荧光检测器的激发波长为280nm,发射波长为450Dill。色谱柱为ttypersilODS—C18,柱温40~C,以磷酸(以三乙胺调pH至2.5)-乙腈(82:18)为流动相,流速1.0mL/min,进样量为20肛L。氧氟沙星在乌骨鸡肌肉、肝脏、。肾脏中的平均回收率分别为86.3%、83.2%、82.5%。检测限为10ng/g。当组织中氧氟沙星浓度在10~2000ug/kg范围时,线性关系良好。利用该方法对氧氟沙星在乌骨鸡体内的残留消除规律进行了研究,结果表明,氧氟沙星在鸟骨鸡体内的残留消除速度极为缓慢。40天休药期后,2日龄使用组可在肌肉、肝脏和肾脏中分别检出氧氟沙星146.8“g/kg、20.6“g/kg和437.4μg/kg;28日龄使用组分别检测出193.5ug/kg、45.0μg/kg和425.5μg/kg。2日龄使用组休药78天时(即停药71天),肌肉和肝脏中还能分别检出氧氟沙星11.2μg/kg和12.0μg/kg。实验表明乌骨鸡使用氧氟沙星仅适用于10日龄以下给药并休药70天以上,或不给予该类药物。 相似文献
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89.
Ganesh K Raghavan R Gowda RN Satyanarayana ML Suryanarayana VV 《Tropical animal health and production》2002,34(1):7-17
Hydropericardium hepatitis syndrome in broiler chickens is an acute, infectious disease characterized by high mortality, excess pericardial fluid and multifocal hepatic necrosis. The aetiological agent was purified to homogeneity from infected liver tissues from field outbreaks. Electron-microscopic and serological confirmation of the virus were undertaken and the disease was reproduced experimentally in broiler chicks. The results indicated that an adenovirus, fowl adenovirus serotype 4, was alone responsible for the disease in the materials studied. 相似文献
90.
氟甲砜霉素对实验性鸡巴氏杆菌病的药效研究 总被引:1,自引:0,他引:1
以两倍稀释法测定氟甲砜霉素及甲砜霉素对鸡巴氏杆菌的最小抑菌浓度分别为0.25μg/mL和1.25μg/mL。按10,20和30mg/kg的氟甲砜霉素及30mg/kg的甲砜霉素分别给实验性巴氏杆菌病患鸡肌注,每天给药一次,共5d。药物对鸡巴氏杆菌病的治愈率分别为93.3%,100%,100%和100%,而感染对照组鸡全部死亡。用药组与感染对照组间疗效差异极显著 相似文献