Effect of homocysteine on expression of interleukin-8 mRNA and protein in THP-1 macrophages

AIM: To investigate the effect of homocysteine (Hcy) on expression of interleukin-8 (IL-8) mRNA and protein in THP-1-derived macrophages (THP-1 macrophages). METHODS: Cultured THP-1 monocytes were induced to macrophages by 0.1 μmol/L PMA treatment for 72 hours, then the differentiated THP-1 macrophages were incubated with homocysteine (0.01 mmol/L-0.20 mmol/L) for 24 hours, or with 0.10 mmol/L Hcy for various time up to 48 hours. IL-8 protein in THP-1 supernatants was measured by ELISA, and IL-8 mRNA expression was detected by semiquantitive RT-PCR. RESULTS: Compared with control, Hcy significantly increased the expression of IL-8 protein in a concentration-dependent manner. 0.05 mmol/L, 0.10 mmol/L and 0.20 mmol/L Hcy increased IL-8 production by 1.28 fold, 1.32 fold and 1.55 fold, respectively (P＜0.01). IL-8 production were elevated significantly 3 h after treatment with 0.10 mmol/L Hcy. In addition, Hcy also increased IL-8 mRNA expression in a concentration-and time-dependent manner. CONCLUSION: Hcy may contribute to atherogenesis by inducing IL-8 expression and secretion in THP-1 macrophages.     

Effects of changing PKC activity on proliferation and telomerase expression in human hepatocellular carcinoma cells

AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC.     

秀丽纤杆线虫在寄生性线虫抗原研究中的应用

秀丽纤杆线虫 (Caenorhabditis elegans)是一种模式线虫 ,目前广泛应用于寄生性线虫基因的功能研究 ,并且还被用于捻转血矛线虫等寄生性线虫疫苗侯选抗原基因的表达和耐药性产生机制方面的研究     

猪瘟病毒及其全长cDNA在猪肾细胞中增殖表达特性的比较研究

以猪瘟C-株弱毒疫苗株、经典强毒石门株和C-株全长cDNA为试验材料，以免疫荧光技术为主；结合ELISA和RT-PCR检测技术，对其在培养细胞中的增殖特性和表达规律进行了比较研究。同时筛选猪瘟病毒及其全长cDNA的敏感细胞，探索其增殖表达规律，以便为猪瘟病毒反向遗传操作提供技术方法和可操作的条件。     

不同温度下圆叶决明降解过程红壤可溶性氮及氮水解酶动态变化研究

为了探明圆叶决明（Chamaecrista rotundifolia）降解过程果园红壤供氮水平的变化规律，本研究采用模拟培养试验，研究15℃和25℃培养果园红壤硝态氮、铵态氮、可溶性总氮、可溶性有机氮含量的变化及脲酶、蛋白酶、天冬酰胺酶活性的变化。结果表明，圆叶决明降解过程果园红壤4种可溶性氮含量均显著提高，且在培养140 d达到最大值，25℃培养的效果更佳。其中，可溶性总氮、可溶性有机氮和硝态氮含量随时间的动态变化可用三次曲线方程来拟合。圆叶决明降解过程还能显著提高脲酶、蛋白酶和天冬酰胺酶的活性，其中蛋白酶活性和脲酶活性的变化可用指数方程和三次曲线方程来拟合。本研究认为圆叶决明降解过程可提高果园红壤可溶性氮含量，因此可通过翻压圆叶决明提高土壤的供氮水平。     

Response of labile organic C and N pools to plastic film removal from semiarid farmland soil

To examine the effects of plastic film removal on grain yield and soil organic matter (SOM), a spring maize (Zea may L.) field experiment was conducted for 5 yr at Changwu Agricultural and Ecological Experimental Station of Northwest China. Compared with traditional plastic film mulching during entire growing stages (FM), plastic film removal at the silking stage (RM) resulted in a 6.3% higher average maize yield. Under the RM treatment, soil organic carbon and total nitrogen significantly increased after the 5‐yr cultivation in the 0‐ to 20‐cm layer. Significant increases in extractable organic C (EOC), KMnO₄‐oxidizable C (KMnO₄‐C) and C management index (CMI) in the 0‐ to 20‐cm layer, and light fraction organic C and EOC in the 20‐ to 40‐cm layer were observed in response to plastic film removal after the 1‐yr treatment; the responses were more significant after 5 yr. Under the RM treatment, significant increases in microbial biomass C, light fraction organic N, extractable organic N, KMnO₄‐C and CMI were also observed after five years in the 20‐ to 40‐cm layer. Moreover, KMnO₄‐C and EOC were much more sensitive than other labile SOM fractions to the application of RM, even after only 1 yr of cultivation. Therefore, compared with mulching for the whole growing season, plastic film removal at the maize silking stage is an effective option for increasing yields and enhancing SOM concentration and soil sustainability in the regions with semiarid monsoon climates that have sufficient rainfall during maize reproductive stages.     

一种简易的花房温湿度无线自动监控系统

花房内的温湿度变化直接影响到花卉的健壮生长,以C8051F330为核心设计一款简易的花房温湿度无线自动监控系统,可对花房内的温湿度进行实时自动监控,当花房内温湿度指标超过设定值时,系统发出声光报警。该系统温度测量范围为-55~125℃,精度为±4℃;相对湿度测量范围为10%~90%,精度为±5%。经测试,系统运行稳定,...     

[CO2]升高对粮食作物影响的研究进展

自工业革命以来,由人类活动引起的大气CO_2浓度([CO_2])不断攀升,正驱动着全球气候变化,对全球农业产生重大影响。本文归纳总结了目前作物对高[CO_2]响应的主要研究技术手段,以及作物对高[CO_2]响应的机理研究,并进一步梳理了当前全球关于[CO_2]升高对作物产量和营养品质影响的研究。结果表明:相比封闭式或半封闭式环境控制试验系统,开放式试验系统(如开放式CO_2控制系统FACE)由于其能更加真实地模拟自然条件下作物对未来高[CO_2]的响应和适应情况,被公认为是目前研究作物对高[CO_2]响应的最理想手段。[CO_2]增高会增加C3作物光合速率、生物量和产量,在一定程度上缓解气候变化对农作物产生的负面影响,但是作物对大气[CO_2]的升高存在光合适应现象,当作物长期暴露在高[CO_2]条件下时,高[CO_2]对作物的促进作用会逐渐减缓。近10年的FACE试验发现,对高[CO_2]出现高应答的水稻品种,其光合速率和产量在高[CO_2]下的增加幅度比早期的主要粮食作物FACE试验结果平均高出两倍。此外,高[CO_2]会明显降低大部分非豆科C3作物中蛋白质和矿物质(如锌、铁)以及部分维生素的含量,加剧目前全球约2亿人由于维生素和矿物质元素等营养缺乏导致的健康问题。如何充分利用未来高[CO_2]实现高增产的同时,减缓粮食养分下降的负面影响,是迫切需要解决的科学问题。     

高温胁迫下蝴蝶豆的生理响应和耐热性评价

以自然高温胁迫下的11份蝴蝶豆为材料,研究叶片厚度、叶绿素值、叶片含水量、电导率值和丙二醛(MDA)含量等外观和生理指标与对照的差异,隶属函数分析结果表明:在高温胁迫下,材料C4、C6、C11的生长表现较好,在叶绿素含量、质膜透性、叶片含水量、MDA含量中的综合反应均较好,相比其它8个材料具有较好的相对耐高温特性,C1和C5的综合反应较弱,为热敏材料.     

可燃气体检测与报警装置设计

文章介绍了以STC12C5201AD为核心的可燃气体检测与报警装置设计.该装置用催化型可燃气体传感器将气体浓度转换为对应的电压值,然后采用A/D进行采集和标度变换,最后由单片机进行信息处理,判断浓度是否超标,并控制报警动作.