莫氏兰的品种选育和栽培技术研究

对从新加坡引进的莫氏兰进行适应性栽培。筛选出Mokara Adisa、M. Red、M. Dinah Shore、M. Chittl Orange Spot、M. Chao Praya Gold、M. Chao Praya Boy、M. Chark Kuan Pink、M. Kitiya共8个适宜在海南省三亚市栽培的莫氏兰优良切花品种，并对莫氏兰优良品种的主要栽培技术进行介绍。     

331份四川小麦品种(系)在甘肃陇南抗条锈性表现及利用价值

2014年-2017年度先后对331份四川省小麦生产品种(系)在温室进行苗期人工接种条锈菌混合菌鉴定和甘谷试验站大田成株期分别接种CYR32、CYR33、CYR34、G22-14、中4-1和混合菌鉴定,同时在甘谷试验站和汪川良种场两地进行自然诱发鉴定。结果发现:在人工接种条件下,苗期、成株期表现抗病的分别有‘XK201465’等36份和‘XK20132’等72份材料,分别占10.88%和21.75%;有‘XK62483’等11份材料全生育期表现抗病,占3.32%;两地三年自然诱发鉴定发现,仅有‘XK20132’等5份材料在两地均表现抗病,有‘川农17’等30份材料具有慢条锈性。对供鉴材料在甘肃陇南的利用前景进行了分析。     

Effect of aging at different temperatures on LAOS properties and secondary protein structure of hard wheat flour dough

The effects of aging from t = 0–108 h at two different temperatures (4 and 25 °C) on the non-linear viscoelastic rheological properties and secondary protein structure of hard wheat flour dough (HWD) were investigated using large amplitude oscillatory shear tests (LAOS) coupled with Fourier transform infrared spectroscopy (FTIR) and SDS-PAGE. Storage (G') and loss (G'') moduli rapidly decreased during aging at 25 °C. Subjecting HWD to progressively longer aging times at 25 °C caused dramatic changes in the non-linear viscoelastic properties demonstrated by strain softening (negative values of e₃/e₁) and shear thinning (negative values of v₃/v₁) behavior. Elastic Lissajous curves of the unaged control dough showed clockwise turn and wider elliptical trajectories as dough aging proceeds especially at higher temperatures. Other non-linear LAOS parameters (G'_M-G''_L, η'_M-η'_L, S and T) supported that aging process at higher temperature caused a progressive change in dough structure from strain stiffening to strain softening behavior while dough samples aged at 4 °C showed fairly close behavior with the control dough sample. FTIR spectra indicated that the relative content of β-sheet and β-turn structures decreased while the content of α-helix structure increased for all dough samples as a result of dough aging. SDS-PAGE results supported the breakdown of high molecular weight (HMW) and low molecular weight (LMW) glutenin subfractions. Aging at the higher temperature of 25 °C decreased the HMW/LMW ratio from 0.77 to 0.59, while the ratio was 0.73 for the dough aged at 4 °C which is fairly close to the control sample. Our results show that the degradation rate of gluten/starch network was triggered by aging at higher temperature, longer aging time, and natural fermentation which resulted in increasing acidity and increase in endogenous proteolytic and amylolytic activity, and also increasing gluten solubility and break down of intermolecular disulfide bonds at acid pH.     

镉胁迫下猕猴桃对镉的吸收及转运

在对湖南省猕猴桃成年果园8个猕猴桃品种(金魁、金艳、翠玉、东红、红阳、丰悦、魁蜜、米良一号)镉含量调查分析的基础上，选取镉富集能力有差异的米良一号与金艳猕猴桃进行镉胁迫(5、10、15、20 mg/kg)下对镉的吸收、转运的差异研究。结果表明：成年果园8个猕猴桃品种在土壤中度镉污染(0.9 mg/kg)时，果实镉含量均未超过国家标准值(0.05 mg/kg)；猕猴桃果实成熟期镉含量最高；镉胁迫下，米良一号和金艳镉含量随镉处理浓度增大而上升，且在镉浓度较低情况下富集能力较强；根的镉分配系数最大(＞0.90)，果实的最小，说明猕猴桃主要由根部吸收累积镉；米良一号整株吸收和积累镉量显著高于金艳，但金艳对镉的转移能力强于米良一号，金艳果实镉含量高于米良一号。     

Development and Application of Cotton InDel Markers Based on High Throughput Sequencing

[Objective] Dimorphic InDel markers can be used for cotton variety identification and purity detection, to improve the accuracy and efficiency of cotton seed testing, and to play a role in molecular breeding of cotton. [Method] Based on the whole genome sequencing of 121 cotton varieties from different sources, the InDel markers with high polymorphism were developed according to polymorphism information content(PIC) and were applied in the genetic distance analysis and cluster analysis by using 66 cotton varieties in China. [Result] Totally 10 967 InDel were identified based on the next generation sequencing data of 121 cotton varieties. Among the 85 pairs of InDel primers synthesized, 64 were selected including 35 from At group and 29 from Dt group. The minimum average allelic frequency(MAF) of At and Dt chromosomes were 0.45 and 0.32, respectively, while the PIC were 0.49 and 0.40, respectively. The genetic distances of the 66 cotton varieties ranged from 0.04 to 0.65 centimorgan (cM), with an average of 0.39 cM. The two varieties with the largest genetic distance were Simian 3 and CCRI 36, and the two varieties with the smallest genetic distance were Xumian 18 and Xuza 3. [Conclusion] The 64 cotton dimorphic InDel markers can effectively reveal the relationships among varieties based on the genetic distance, and distinguish cotton varieties from different sources, which has certain theoretical significance and application value.     

赤峰市苦荞麦品种比较试验初报

为筛选赤峰市适宜种植的苦荞品种,2011年在赤峰市进行9个苦荞麦品种比较试验.结果表明：西荞1号、黔苦3号、苦荞04-46、川荞1号、黔苦5号、晋荞麦2号、西荞2号、六苦2081产量均比对照CK-10-2高,适宜当地推广种植.     

小麦面粉中赭曲霉毒素A的CdSe/ZnS荧光量子点免疫探针检测

构建一种基于荧光量子点免疫探针的小麦面粉中赭曲霉毒素A(OTA)的检测方法。以EDC为活化剂将CdSe/ZnS半导体荧光量子点与OTA单克隆抗体偶联,制备OTA检测探针并评价其检测性能。结果表明:制备的检测探针在3d保存期内具有良好的稳定性。当OTA质量浓度为0.1~0.9μg/L时,检测探针的荧光强度与OTA质量浓度呈线性关系,其相关系数为0.996 3,检出限为0.017 7μg/L。与国标法对照的结果说明,此方法具有可行性。小麦面粉中的加标回收试验结果显示,OTA的回收率为96.0%~102.7%,相对标准偏差不超过4.1%,说明准确性良好。可见,OTA的CdSe/ZnS荧光量子点免疫探针检测方法操作简单、准确度高、结果稳定,有望应用于小麦面粉中OTA的检测。     

Impact of different crop rotations and tillage systems on weed infestation and productivity of bread wheat

Crop rotation and tillage systems have important implications for weed infestation and crop productivity. In this study, five tillage systems viz. zero tillage (ZT), conventional tillage (CT), deep tillage (DT), bed sowing (60/30 cm with four rows; BS₁) and bed sowing (90/45 cm with six rows; BS₂) were evaluated in five different crop rotations viz. fallow-wheat (FW), rice-wheat (RW), cotton-wheat (CW), mungbean-wheat (MW) and sorghum-wheat (SW) for their effect on weed infestation and productivity of bread wheat. Interaction between different tillage practices and cropping systems had significant effect on density and dry biomass of total, broadleaved and grass weeds, agronomic and yield-related traits, and grain yield of bread wheat. The un-disturbed soils (ZT) under fallow-wheat or mungbean-wheat rotations favoured the weed prevalence (a total weed dry biomass of 72.4–109.6 and 105.6–112.1 g m⁻² in first and second year, respectively). Contrary to this, the disturbed soils (CT, DT, BS₁ and BS₂) had less weed infestation with either of the rotations (a total weed biomass of 0.4–7.1 and 1.1–5.4 g m⁻² in first and second year, respectively). Sorghum-wheat rotation had strong suppressive effect on weed infestation in all tillage systems. The impact of crop rotation was more visible during second year of experimentation. Bed sown wheat (BS₁ and BS₂) in mungbean-wheat rotation had the highest wheat grain yield (6.30–6.47 t ha⁻¹) compared to other tillage systems in different crop rotation combinations.     

籼型耐高温三系不育系巨风2A的选育

以优质常规材料9526为母本,宜陵1B为父本杂交,优选后代与宜陵1A回交而育成的早熟中籼型三系不育系巨风2A,具有耐高温、高异交率、高配合力,所配杂交稻熟色好等优点,应用前景广泛。2014年8月通过了湖北省农作物品种审定委员会的现场鉴定,2015年通过了湖北省审定。     

田间春播法鉴定小麦新品系冬春性

[目的]鉴定小麦新品系冬春性,为小麦品系选育提供帮助。[方法]通过田间春播法对2015年新选育的21个小麦品系进行冬春性分析。[结果]3个播期相互参考,相互印证,参加鉴定的21个品系,最终判定为1个弱冬性,13个半冬性,4个弱春性,3个春性;冬性类14个,春性类7个,冬性类品系占品系总数的66.7%。[结论]田间春播可以作为小麦冬春性鉴定的有效方法。