长期定位施不同氮源有机肥替代部分含氮化肥对陇东旱塬冬小麦产量和水分利用效率的影响

在陇东黄土旱塬覆盖黑垆土大田,利用陇鉴301进行了连续10年的定位试验,观测长期施用不同氮源有机肥(发酵有机肥、农家肥)或小麦秸秆还田各处理对冬小麦产量相关性状和水分利用效率的影响。结果显示,小麦产量和水分利用效率年际间变化较大,与降雨量有直接关系;不同有机氮源替代部分含氮化肥处理对提高冬小麦产量和水分利用效率具有明显作用。在干旱、平水和丰水年型,较不施肥对照分别增产53.1%~103.7%、40.3%~79.3%和73.1%~94.8%,较单施化肥对照分别增产6.6%~41.8%、7.0%~36.8%和-2.9%~9.3%。以发酵有机肥做替代物处理的产量和水分利用效率最高,增产幅度最大,10年平均产量较不施肥和单施化肥对照分别增加88.9%和25.4%,水分利用效率和边际水分利用率也最高,分别为10.8 kg mm~(-1) hm~(-2)和1.03 kg m~(-3);并且产量构成因素和植株生理指标也优于其他处理。因此,发酵有机肥是陇东半湿润偏旱雨养农业区(年降水量约550 mm)冬小麦生产上替代部分含氮化肥的首选有机氮源。     

新时代土壤化学前沿进展与展望

土壤化学是重要的土壤学基础分支学科。在回顾了土壤化学发展历程的基础上,梳理了土壤化学的四个前沿交叉方向,并展望了土壤化学与其他相关学科的交叉发展趋势,以期寻求新的学科增长点。土壤化学经历了从恒电荷到可变电荷土壤学说演变,我国在土壤电化学、根际土壤化学、土壤化学-物理-微生物界面反应等方向逐步领跑。新时代中国已经发展成为国际土壤化学的研究中心之一,尤其在土壤化学与微生物学、地球化学、矿物学、环境化学等交叉领域取得了突破性发展。同时,发展并运用同步辐射、微流控联用光谱能谱、高分辨显微镜、光谱电化学等实时、原位、高精度研究方法,推动土壤化学研究取得了长足的进步。新时代的土壤化学具有三个重要发展趋势,首先系统揭示地球表层系统中物质循环与能量交换的土壤化学机制,实现"0到1"的土壤化学原创性成果的突破;其次需要综合运用地球表层系统理论,从多界面、多要素、多过程的"三多"交互耦合;再次,需要加强与地球宜居性这一人类重大命题的交叉融合,为生态文明建设、土壤污染防治攻坚战、全球变化等国家重大需求提供理论支持。     

Short time effects of biological and inter-row subsoiling on yield of potatoes grown on a loamy sand,and on soil penetration resistance,root growth and nitrogen uptake

Soil compaction, especially subsoil compaction, in agricultural fields has increased due to widespread use of heavy machines and intensification of vehicular traffic. Subsoil compaction changes the relative distribution of roots between soil layers and may restrict root development to the upper part of the soil profile, limiting water and mineral availability. This study investigated the direct effects of inter-row subsoiling, biological subsoiling and a combination of these two methods on soil penetration resistance, root length density, nitrogen uptake and yield. In field experiments with potatoes in 2013 and 2014, inter-row subsoiling (subsoiler) and biological subsoiling (preceding crops) were studied as two potential methods to reduce soil penetration resistance. Inter-row subsoiling was carried out post planting and the preceding crops were established one year, or in one case two years, prior to planting. Soil resistance was determined with a penetrometer three weeks after the potatoes were planted and root length density was measured after soil core sampling 2 months after emergence. Nitrogen uptake was determined in haulm (at haulm killing) and tubers (at harvest). Inter-row subsoiling had the greatest effect on soil penetration resistance, whereas biological subsoiling showed no effects. Root length density (RDL) in the combined treatment was higher than in the separate inter-row and biological subsoiling treatments and the control, whereas for the separate inter-row and biological subsoiling treatments, RLD was higher than in the control. Nitrogen uptake increased with inter-row subsoiling and was significantly higher than in the biological subsoiling and control treatments. However, in these experiments with a good supply of nutrients and water, no yield differences between any treatments were observed.     

Evaluating biodegradability of soil organic matter by its thermal stability and chemical composition

The stability of soil organic matter (SOM) as it relates to resistance to microbial degradation has important implications for nutrient cycling, emission of greenhouse gases, and C sequestration. Hence, there is interest in developing new ways to quantify and characterise the labile and stable forms of SOM. Our objective in this study was to evaluate SOM under widely contrasting management regimes to determine whether the variation in chemical composition and resistance to pyrolysis observed for various constituent C fractions could be related to their resistance to decomposition. Samples from the same soil under permanent pasture, an arable cropping rotation, and chemical fallow were physically fractionated (sand: 2000-50 μm; silt: 50-5 μm, and clay: <5 μm). Biodegradability of the SOM in size fractions and whole soils was assessed in a laboratory mineralization study. Thermal stability was determined by analytical pyrolysis using a Rock-Eval pyrolyser, and chemical composition was characterized by X-ray absorption near-edge structure (XANES) spectroscopy at the C and N K-edges. Relative to the pasture soil, SOM in the arable and fallow soils declined by 30% and 40%, respectively. The mineralization bioassay showed that SOM in whole soil and soil fractions under fallow was less susceptible to biodegradation than that in other management practices. The SOM in the sand fraction was significantly more biodegradable than that in the silt or clay fractions. Analysis by XANES showed a proportional increase in carboxylates and a reduction in amides (protein) and aromatics in the fallow whole soil compared to the pasture and arable soils. Moreover, protein depletion was greatest in the sand fraction of the fallow soil. Sand fractions in fallow and arable soils were, however, relatively enriched in plant-derived phenols, aromatics, and carboxylates compared to the sand fraction of pasture soils. Analytical pyrolysis showed distinct differences in the thermal stability of SOM among the whole soil and their size fractions; it also showed that the loss of SOM generally involved preferential degradation of H-rich compounds. The temperature at which half of the C was pyrolyzed was strongly correlated with mineralizable C, providing good evidence for a link between the biological and thermal stability of SOM.     

Environmental stress response limits microbial necromass contributions to soil organic carbon

The majority of dead organic material enters the soil carbon pool following initial incorporation into microbial biomass. The decomposition of microbial necromass carbon (C) is, therefore, an important process governing the balance between terrestrial and atmospheric C pools. We tested how abiotic stress (drought), biotic interactions (invertebrate grazing) and physical disturbance influence the biochemistry (C:N ratio and calcium oxalate production) of living fungal cells, and the subsequent stabilization of fungal-derived C after senescence. We traced the fate of ¹³C-labeled necromass from ‘stressed’ and ‘unstressed’ fungi into living soil microbes, dissolved organic carbon (DOC), total soil carbon and respired CO₂. All stressors stimulated the production of calcium oxalate crystals and enhanced the C:N ratios of living fungal mycelia, leading to the formation of ‘recalcitrant’ necromass. Although we were unable to detect consistent effects of stress on the mineralization rates of fungal necromass, a greater proportion of the non-stressed (labile) fungal necromass C was stabilised in soil. Our finding is consistent with the emerging understanding that recalcitrant material is entirely decomposed within soil, but incorporated less efficiently into living microbial biomass and, ultimately, into stable SOC.     

Development of a kompetitive allele‐specific PCR marker for selection of the mutated Wx‐D1d allele in wheat breeding

Waxy (Wx) protein is a key enzyme for synthesis of amylose in endosperm. Amylose content in wheat grain influences the quality of end‐use products. Seven alleles have been described at the Wx‐D1 locus, but only two of them (Wx‐D1b, Wx‐D1e) were genotyped with codominant markers. The waxy wheat line K107Wx1 developed by treating ‘Kanto 107’ seeds with ethyl methanesulphonate carries the Wx‐D1d allele. However, no molecular basis supports this nomenclature. In the present study, DNA sequence analysis confirmed that a single nucleotide polymorphism in the sixth exon of Wx‐D1 changed tryptophan at position 301 into a termination codon. Based on this sequence variation, a PCR‐based KASP marker was developed to detect this point mutation using 68 BC₈F₁ plants and 297 BC₈F₂ lines derived from the cross ‘Ningmai 14’*9/K107Wx1. Combined with codominant markers for the Wx‐A1 and Wx‐B1 alleles, waxy and non‐waxy near‐isogenic lines were distinguished. The KASP marker was efficient in identifying the mutant allele and can be used to transfer waxiness to elite lines.     

Contrasting development of soil microbial community structure under no-tilled perennial and tilled cropping during early pedogenesis of a Mollisol

A range of agricultural practices influence soil microbial communities, such as tillage and organic C inputs, however such effects are largely unknown at the initial stage of soil formation. Using an eight-year field experiment established on exposed parent material (PM) of a Mollisol, our objectives were to: (1) to determine the effects of field management and soil depth on soil microbial community structure; (2) to elucidate shifts in microbial community structure in relation to PM, compared to an arable Mollisol (MO) without organic amendment; and (3) to identify the controlling factors of such changes in microbial community structure. The treatments included two no-tilled soils supporting perennial crops, and four tilled soils under the same cropping system, with or without chemical fertilization and crop residue amendment. Principal component (PC) analysis of phospholipid fatty acid (PLFA) profiles demonstrated that microbial community structures were affected by tillage and/or organic and inorganic inputs via PC1 and by land use and/or soil depth via PC2. All the field treatments were separated by PM into two groups via PC1, the tilled and the no-tilled soils, with the tilled soils more developed towards MO. The tilled soils were separated with respect to MO via PC1 associated with the differences in mineral fertilization and the quality of organic amendments, with the soils without organic amendment being more similar to MO. The separations via PC1 were principally driven by bacteria and associated with soil pH and soil C, N and P. The separations via PC2 were driven by fungi, actinomycetes and Gram (−) bacteria, and associated with soil bulk density. The separations via both PC1 and PC2 were associated with soil aggregate stability and exchangeable K, indicating the effects of weathering and soil aggregation. The results suggest that in spite of the importance of mineral fertilization and organic amendments, tillage and land-use type play a significant role in determining the nature of the development of associated soil microbial community structures at the initial stages of soil formation.     

不同林龄柠条锦鸡儿土壤有效磷的变化

研究柠条锦鸡儿(Caragana Microphylla Lam.)林地不同土层深度土壤有效磷的变化规律,为进一步深入研究北方农牧交错带土壤性质及地区植被建设提供依据。笔者通过野外调查和数据处理,对赤峰市敖汉旗风沙土区不同林龄(5、11、17、23、29年)柠条锦鸡儿不同土层的土壤有效磷进行了分析。研究表明:相同林龄不同立地条件的土壤有效磷含量随土层厚度的增加呈现减小的趋势;相同林龄不同立地条件的土壤有效磷含量大小表现为丘间低地阴坡阳坡;相同立地条件不同林龄的土壤有效磷含量随林龄的增加而增加。柠条锦鸡儿的种植有利于土壤性质向良性方向发展。     

滨海盐碱地棉花秸秆还田对土壤理化性质及棉花产量的影响

在滨海盐碱地定点设置连续3年棉花秸秆还田和未还田2个处理,研究其对0~60 cm土层土壤理化性质和棉花产量的影响。结果表明,连续3年棉花秸秆还田显著降低0~30 cm土层土壤容重和0~10 cm土层0.25 mm土壤微团聚体含量;显著提高0~10 cm土层5 mm土壤大团聚体含量。在0~20 cm土层,3年秸秆还田显著提高棉花播前和各生育阶段土壤有机质、硝态氮、铵态氮和速效钾含量,平均分别比对照提高13.45%、18.57%、22.80%和22.57%;降低土壤速效磷和含盐量,平均分别比对照降低18.29%和16.59%。在20~40 cm土层,3年秸秆还田显著提高土壤硝态氮和铵态氮含量,平均比对照提高37.20%和31.62%;显著降低土壤含盐量,平均比对照降低19.06%。在40~60 cm土层,3年秸秆还田显著提高土壤硝态氮和铵态氮含量,平均分别比对照提高38.26%和24.83%。3年棉花秸秆还田分别比未还田显著提高棉花籽棉产量11.57%、19.01%和13.24%和皮棉产量18.56%、19.78%和18.73%,但对棉花单铃重和衣分无显著影响。     

测墒补灌深度对济麦22冠层光截获和荧光特性及籽粒产量的影响

测墒补灌是近年开发的一种小麦节水栽培新技术,水分管理的土层深度是该技术的关键因素之一。本研究以济麦22为试验品种,于2013—2014和2014—2015年度在山东兖州进行大田试验,设置4个测墒补灌土层深度,补灌至目标土层拔节期相对含水量70%和开花期相对含水量75%,以定量灌溉(拔节期和开花期各灌水60 mm)和全生育期不灌水处理为对照,通过测定花后0~30 d灌浆阶段小麦冠层光截获特性、群体光合速率、旗叶荧光特性,以及最终籽粒产量和水分利用效率,以明确测墒补灌达到增产的光合基础及最佳土层。当补灌土层为0~20 cm时,灌水量为50.1~51.2 mm,小麦叶面积指数、冠层光合有效辐射截获量、冠层光截获率和群体光合速率,以及旗叶实际光化学效率(ΦPSII)和最大光化学效率(Fv/Fm)在各灌水处理中最低;补灌土层为0~40 cm时,灌水量为73.1~93.1 mm,上述前4项指标比补灌深度20 cm时依次提高6.0%~42.4%、8.5%~27.9%、6.7%~14.5%、11.0%~14.6%,同时旗叶ΦPSII和Fv/Fm亦显著提高;补灌深度加大至60 cm(灌水量87.5~105.4 cm)和80 cm(灌水量101.8~115.0 cm)时,这些指标无显著增加。与光合特性相关指标一致,籽粒产量也表现为补灌深度大于40 cm的3个处理间无显著差异,且与定量灌溉对照无显著差异,但都显著高于补灌深度20 cm处理。在本试验条件下,对0~40 cm土层实施测墒补灌,较定量灌溉减少用水26.9~46.9 mm,水分利用效率提高16.2%~16.7%,灌溉效益增加34.0%~68.1%,说明在类似生态条件下,中穗型小麦品种济麦22测墒补灌节水栽培技术的目标土层为0~40 cm。