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991.
利用花粉管通道法将兔防御素NP-1基因导入小麦的研究 总被引:4,自引:3,他引:4
兔防御素NP-1是抗性谱最广的防御素之一。为提高小麦的抗病虫能力,培育优质高抗的小麦品种,采用花粉管通道法将兔防御素NP-1基因导入优质小麦品种G8901。对得到的193株T0植株进行抗除草剂筛选,获得4株抗性植株。通过PCR及PCR-Southern杂交,证实了NP-1基因已经整合到转基因植株的基因组中,并稳定遗传到T1。田间抗病虫鉴定结果表明,这些植株对于白粉病、叶锈和条锈病有较强的免疫力和抗性,但对于蚜虫的抗性没有明显提高。 相似文献
992.
The importance of reduced tillage in sustainable agriculture is well recognized. Reduced-tillage practices (which may or may
not involve retention of crop residues) and their effects differ from those of conventional tillage in several ways: soil
physical properties; shifts in host–weed competition; soil moisture availability (especially when sowing deeply or under stubble);
and the emergence of pathogen populations that survive on crop residues. There may be a need for genotypes suited to special
forms of mechanization (e.g. direct seeding into residues) and to agronomic conditions such as allelopathy, as well as specific
issues relating to problem soils. This article examines issues and breeding targets for researchers who seek to improve crops
for reduced-tillage systems. Most of the examples used pertain to wheat, but we also refer to other crops. Our primary claim
is that new breeding initiatives are needed to introgress favourable traits into wheat and other crops in areas where reduced
or zero-tillage is being adopted. Key traits include faster emergence, faster decomposition, and the ability to germinate
when deep seeded (so that crops compete with weeds and use available moisture more efficiently). Enhancement of resistance
to new pathogens and insect pests surviving on crop residues must also be given attention. In addition to focusing on new
traits, breeders need to assess germplasm and breeding populations under reduced tillage. Farmer participatory approaches
can also enhance the effectiveness of cultivar development and selection in environments where farmers’ links with technology
providers are weak. Finally, modern breeding tools may also play a substantial role in future efforts to develop adapted crop
genotypes for reduced tillage. 相似文献
993.
The height-reducing gene Rht8 was introduced into Italian wheats by breeder Nazareno Strampelli from the Japanese landrace Akakomugi, and has been widely
used in wheats adapted to southern and eastern European conditions. Following identification of a close linkage to Rht8, microsatellite marker Gwm261 has been used extensively to screen large numbers of diverse international germplasm. A 192bp allele at this locus has been
taken as “diagnostic” for Rht8 and used to infer the international distribution of Rht8. In this paper, we report several instances of cultivars and mapping populations that vary for the presence of the 192bp
allele at the Xgwm261 locus (Xgwm261
192
), but with no associated reduction in height, suggesting a lack of association with Rht8. For instance, in the population derived from a cross between Sunco (Rht-B1b, Xgwm261
165
) and Tasman (Rht-D1b, Xgwm261
192
), there were significant height differences associated with the segregation of Rht-B1b and Rht-D1b, but no height differences between Xgwm261 genotypes. Similar results were obtained in a population derived from the cross between Molineux (Rht-B1b, Xgwm261
192
) and Trident (Rht-D1b Xgwm261
208
). In contrast, the cross between Trident and Chuanmai 18 (Xgwm261
192
) gave significant height effects at both the Rht-D1 and Xgwm261 loci, with no epistatic interaction between loci. Chuanmai 18 is closely related to the Strampelli wheat Mara (ancestrally
derived from Akakomugi) and is therefore likely to carry Rht8. The old Japanese cultivar Norin 10, used by Norman Borlaug to introduce Rht-B1b and Rht-D1b into Mexican wheats, also has a 192bp allele at the Xgwm261 locus, and the sequence of the amplified product is identical to that of Akakomugi. We suggest that the widespread use of
Norin 10-derived germplasm during and after the Green Revolution introduced a second haplotype into international germplasm,
in which Xgwm261
192
has no association with Rht8. Therefore, the presence of Xgwm261
192
is only indicative of Rht8 in wheat cultivars that have inherited this allele from Akakomugi or a Strampelli wheat ancestor. 相似文献
994.
995.
996.
本研究以克隆所得杜仲(Eucommia ulmoides)EuGT2基因为基础,利用基因重组技术构建原核表达载体pMCSG19-EuGT2。重组载体转化到E.coli Rosetta(DE3)中,用0.05 mmol/L异丙基β-D-硫代半乳糖苷(IPTG)在37℃下诱导6 h。使用镍-亚氨基二乙酸(Ni-IDA)亲和层析柱进行纯化,并用15%十二烷基硫酸钠-聚丙烯酰胺(SDS-PAGE)凝胶电泳进行检测。研究结果表明,杜仲EuGT2基因在E.coli Rosetta(DE3)中得到成功表达,重组蛋白表达形式为部分包涵体、部分可溶性蛋白。经SDS-PAGE凝胶电泳检测,在相对分子质量约56 kD处有1条特异性蛋白条带,经质谱鉴定该特异性蛋白条带确为杜仲EuGT2蛋白。以上研究结果为后续研究杜仲糖基转移酶的功能提供了科学依据。 相似文献
997.
采用酸性聚丙烯酰胺凝胶电泳(A-PAGE),分析了山东省种植面积较大的37个小麦品种醇溶蛋白Gli-1和Gli-2位点等位基因的组成特点。结果表明,山东小麦在醇溶蛋白Gli-1 (Gli-A1、Gli-B1和Gli-D1) 和Gli-2 (Gli-A2、Gli-B2和Gli-D2) 位点存在多样性,共鉴定出58个等位基因,出现频率较高的有6个,分别为Gli-A1a (48.6 %)、Gli-B1l (35.1%)、Gli-D1k (35.1%)、Gli-A2b (35.1%)、Gli-B2g (35.1%)和Gli-D2a (29.7%),其中Gli-B1l出现频率较高,表明1BL/1RS易位系在山东小麦中存在比较普遍。醇溶蛋白6个主要位点的遗传变异系数较高,平均为0.7930,变幅为0.7297~0.8269,其中Gli-D2位点遗传多样性最高,Gli-A1最低。对具有优质醇溶蛋白等位基因Gli-B1b或Gli-A2b的品种进行了高分子量谷蛋白亚基的组成分析,表明烟农15、烟优361、山农98-1和山农93-52同时含有优质谷蛋白5+10亚基,在小麦育种中可利用这些优质亚基基因。 相似文献
998.
研究刺山柑提取物(CSE)对小鼠耳廓微循环的影响,为改善微循环障碍性疾病提供实验依据。将正常小鼠50只随机分为5组(空白对照组,阳性对照组,CSE 1.25、2.5、5 mg/(10g·天)组。每天给药一次,连续灌胃7天。用WX-6微循环显微仪分别于末次给药后六个时间点即0.5、1.0、1.5、2.0、2.5、3.0 h于给药前耳廓相同部位测量耳廓微循环微动脉、微静脉血管口径及毛细血管开放的数目。与空白组比较,CSE低、中、高剂量组能明显增加耳廓微血管管径及毛细血管开放数目(P0.05),增强作用与作用时间及剂量成正相关,以CSE高剂量更为显著。CSE对正常小鼠微循环具有改善作用。 相似文献
999.
1000.
氮硫交互作用对小麦苗期生长和养分吸收的影响 总被引:15,自引:1,他引:15
通过基质盆栽冬小麦,研究了氮硫交互作用对小麦苗期生长及养分吸收的影响。研究结果表明,氮、硫对苗期小麦的生长都有一定影响,氮的增产效果远大于硫,当氮肥用量为200mg/盆时,小麦生物量的最大增产幅度89%;在低氮水平下,硫肥的增产效果最佳,最高可增产125。硫肥的施用可以提高苗期小麦植株体内硫的累积量,在氮素胁迫的情况下最为显著,可提高1倍以上;同时降低植株体内氮的累积,但降低幅度不大。氮素充足时,地上部与地下部生物量之比为2.4-3.9,氮素胁迫条件下,小麦的地上部和地下部的生物量相当,说明氮素养分不足时,小麦合成的有机物质更倾向于分配给根系,促进根系的生长,这可能是小麦抵抗胁迫环境条件的生理机制之一。 相似文献