黑穗醋栗品种亲缘关系的ISSR分析

 采用ISSR标记技术对39个黑穗醋栗品种的亲缘关系进行分析。结果表明：13条ISSR引物扩增的总带数（A）从3到11条不等,平均扩增出6.77条带,平均多态性带百分比（P）为64.37%。聚类分析显示,在相似性系数为0.8496处将39个黑穗醋栗品种分为5组。第Ⅰ组包含8个起源国家的品种,说明世界各国之间基因交流较为频繁。‘Ben Lomond’和‘Kantata’同其他品种的遗传距离较远,两者又有诸多优良农艺性状,可作为黑穗醋栗的优良育种材料。     

Pathogenesis-related (PR)-proteins: Chitinase and β-1,3-glucanase in defense mechanism against malformation in mango (Mangifera indica L.)

Mango, the king of fruits in India is cultivated commercially in many tropical and subtropical regions of the world. Undoubtedly, mango malformation is a serious disease affecting mango production in India and many other countries around the world. It is now shown that the malady is inflicted by Fusarium, a fungus, and also that the plants have the capacity to suppress or reduce pathogen attack by inducing the synthesis of antimicrobial metabolites such as chitinase and/or the synthesis of lignin, both of which may enhance plant defense system. The present study was aimed at investigating the variability and relationship between activities of chitinase, β-1,3-glucanase and content of lignin in the leaves using 12 mango cultivars with the different degree of resistance to floral malformation. Results revealed that the activity of chitinase and β-1,3-glucanase in the leaves were significantly high in mango cultivars resistant to malformation (r = −0.90 and r = −0.91, respectively) during the flowering period, whereas lignin content did not show a significant correlation with malformation. The highest activity of chitinase (1.977–2.011 units) and β-1,3-glucanase (80.54–82.06 units) was recorded in resistant mango cultivars Bhadauran and Elaichi. In contrast, these activities were less than 1.010 and 25.21 respectively in highly susceptible mango cultivars such as Amrapali, Eldon and Neelum. Lignin content was highest in resistant cultivar Bhadauran, but it did not show significant relation to the malformation intensity of the cultivars. Thus, leaf chitinase and β-1,3-glucanase may be contributing towards resistance to malformation in mango and that the relative activities of these enzymes can be used as a criterion to predict and screen the mango germplasm and cultivars for resistance to floral malformation.     

不同白菜品种对锌的响应及锌利用效率研究

采用盆栽试验研究了白菜[Brassica campestris L.ssp.Chinensis(L.)Makino]4个品种对不同浓度锌(Zn 0、1、10 mg/kg)的响应.结果表明,白菜的生物量及体内锌含量随锌水平的增加而增加;但白菜品种对锌营养反应的敏感性不同.地上部锌含量、锌积累量和锌吸收效率均以日本华冠(J...     

早实核桃物候期观察与避晚霜品种的筛选

针对北方地区核桃受晚霜危害制约生产效益的问题,在渭河平原中部选择避晚霜品种及适宜的授粉品种,对12个早实核桃品种物候期进行了观察.结果表明:不同早实核桃品种物候期各异.早实核桃各物候期(除展叶期)均与有效积温呈极显著正相关.“辽核3号”、“辽核4号”、“香玲”、“鲁光”、“扎343”、“维纳”、“吐来尔”、“强特勒”为雄先型品种;“西林2号”、“西扶2号”、“温185”、“中林5号”为雌先型品种.“西林2号”、“西扶2号”、“中林5号”、“维纳”、“吐来尔”和“强特勒”雌雄花期部分相遇;“香玲”、“鲁光”、“辽核3号”、“辽核4号”、“温185”、“扎343”雌雄花期不相遇;“西林2号”、“西扶2号”、“香玲”、“鲁光”、“扎343”可为“辽核3号”、“辽核4号”、“温185”授粉;“扎343”和“温185”可以相互授粉.“香玲”、“鲁光”、“扎343”的雌花期基本能避开晚霜危害;“维纳”、“吐来尔”、“强特勒”能避开晚霜危害,是渭北栽培核桃的首选品种.     

Protein heterogeneity in European wheat landraces and obsolete cultivars

Identity and present degree of genetic homogeneity and heterogeneity, respectively of 52 European wheat accessions, maintained in the collection of wheat genetic resources, have been characterized using analyses of glutenins by sodiumdodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). Six of the analyzed wheat accessions were observed to be homogeneous, while 46 (88.5%) of them were heterogeneous in protein profiles. Heterogeneous accessions possessed 2 to 13 different protein lanes. Together, 17 high molecular weight glutenin subunit (HMW-GS) alleles have been found. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1, and Glu-D1 complex loci were 1, 7+9, and 2+12, respectively. However, also low frequented HMW-GS alleles or allelic combinations, such as 7+15, 13+16, 20, 6, 7, and 9 were observed. Furthermore, another new allele encoding HMW glutenin subunit with relative molecular weight 98.6 kDa has been found in one of the lines of the cultivar Eritrospermum 917. The Glu-score in the examined accessions varied in broad range, some of the lines reached the maximum value 10.     

利用作物模拟模型辅助决策小麦新品种推广初探

在田间试验和前人研究的基础上，以比较成熟的CERES-WHEAT模型为研究工具，以我国的主要作物小麦为研究材料，探讨了作物模型在区域试验和良种评价方面的潜在应用。目的在于应用作物模型的动态性和预测性功能，通过利用品种已有生理和遗传参数以及各地的气象、土壤资料，对冬小麦不同品种同一地点和同一品种不同地点的生育期、物候期、产量和产量构成因素进行模拟，并与实测值进行比较，最后对品种的生态适应性进行了综合评价。最终在品种产量表现和生态适应性等方面为品种区域试验和良种推广提供决策的依据，试图尝试用一条新的途径，辅助     

Allelopathy variation in dryland winter wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.) accessions grown on the Loess Plateau of China for about fifty years

In this study, we investigated the genetic variation of allelopathic potential, its grey correlation with important agronomic characters, and rank analysis on allelopathic stability of fifteen wheat accessions commonly grown in arid regions of the Loess Plateau in China. The genetic variation of allelopathic property in dryland winter wheat showed significant differences between accessions. Allelopathic effects exhibited high heritability (55–95%) throughout the life cycle of wheat. Heritability was highest in the tillering stage and weakest in the seed filling stage. Allelopathic potential varied and was discontinuous throughout the wheat life cycle. Grey correlation analysis indicated a close relationship between allelopathic potential dependant on growth phase and agronomic characters. Allelopathic expression during some growth periods induced a partial correlation effect on some important agronomic characters that affected wheat yield. Allelopathic heritability and its degree of influence on yield were more evident in the vegetative growth stage compared to the reproductive stage. A multiple linear regression was built between allelopathic potential during different growth periods and agronomic characters pursued in wheat breeding. Allelopathic potential had a linear effect on production traits cultivated in wheat breeding. When allelopathic intensity varied from 0 to 1 in the reproductive stage, plant height ranged from 44 to 108 cm, spike length from 6.4 to 9.2 cm, number of spikelets with seeds from 13.4 to 21.0, mean seed number per spike from 41.5 to 50.3 and thousand seed weight from 36.2 to 38.3 g. Based on rank analysis, we concluded that there was a synergistic relationship between allelopathic potential in wheat and genetic, chemical and ecological factors. Triticum aestivum L. ‘No 6 Lankao’ and T. aestivum L. ‘No 22 Xiaoyan’ were identified as stable and relatively strong allelopathic accessions, whereas T. aestivum L. ‘Lankao 95–25’ was a stable but relatively weak allelopathic cultivar. Other varieties showed unstable allelopathic potential.     

Root growth and N‐uptake activity of oilseed rape (Brassica napus L.) cultivars differing in nitrogen efficiency

Nitrate‐N uptake from soil depends on root growth and uptake activity. However, under field conditions N‐uptake activity is difficult to estimate from soil‐N depletion due to different loss pathways. We modified the current mesh‐bag method to estimate nitrate‐N‐uptake activity and root growth of two oilseed‐rape cultivars differing in N‐uptake efficiency. N‐efficient cultivar (cv.) ‘Apex' and N‐inefficient cv. ‘Capitol' were grown in a field experiment on a silty clayey gleyic fluvisol near Göttingen, northern Germany, and fertilized with 0 (N₀) and 227 (N₂₂₇) kg N ha^–1. In February 2002, PVC tubes with a diameter of 50 mm were installed between plant rows at 0–0.3 and 0–0.6 m soil depth with an angle of 45°. At the beginning of shooting, beginning of flowering, and at seed filling, the PVC tubes were substituted by PVC tubes (compartments) of the same diameter, but with an open window at the upper side either at a soil depth of 0–0.3 or 0.3–0.6 m allowing roots to grow into the tubes. Anion‐exchange resin at the bottom of the compartment allowed estimation of nitrate leaching. The compartments were then filled with root‐free soil which was amended with or without 90 mg N (kg soil)^–1. The newly developed roots and nitrate‐N depletion were estimated in the compartments after the installing period (21 d at shooting stage and 16 d both at flowering and grain‐filling stages). Nitrate‐N depletion was estimated from the difference between NO ‐N contents of compartments containing roots and control compartments (windows closed with a membrane) containing no roots. The amount of nitrate leached from the compartments was quantified from the resin and has been taken into consideration in the calculation of the N depletion. The amount of N depleted from the compartments significantly correlated with root‐length density. Suboptimal N application to the crop reduced total biomass and seed‐yield formation substantially (24% and 38% for ‘Apex’ and ‘Capitol’, respectively). At the shooting stage, there were no differences in root production and N depletion from the compartments by the two cultivars between N₀ and N₂₂₇. But at flowering and seed‐filling stages, higher root production and accordingly higher N depletion was observed at N₀ compared to N₂₂₇. Towards later growth stages, the newly developed roots were characterized by a reduction of root diameter and a shift towards the deeper soil layer (0.3–0.6m). At low but not at high N supply, the N‐efficient cv. ‘Apex’ exhibited higher root growth and accordingly depleted nitrate‐N more effectively than the N‐inefficient cv. ‘Capitol’, especially during the reproductive growth phase. The calculated nitrate‐N‐uptake rate per unit root length was maximal at flowering (for the low N supply) but showed no difference between the two cultivars. This indicated that the higher N‐uptake efficiency of cv. ‘Apex’ was due to higher root growth rather than higher uptake per unit of root length.     

四川省近10年审定普通玉米品种主要性状分析

对2005-2014年四川省审定的226个玉米品种的产量、8个农艺性状和5个品质性状进行统计分析,并对其变化趋势进行分析.结果表明,10年间四川省审定的玉米品种平均每667 m2产量为536.66 kg,产量优势不突出;平均生育期、平均株高、平均穗长、平均行粒数、平均穗行数、平均穗位高、平均百粒重、平均出籽率分别为123.26 d、262.96 cm、19.54 cm 、36.45粒、16.32行、104.92 cm、30.78 g、85.35％,年际间差异不大;平均容重为736.66g/L,高于国家一级标准;平均粗蛋白质含量、平均粗淀粉含量、平均粗脂肪含量、平均赖氨酸含量分别为9.99％、73.93％、4.53％、0.31％,整体营养水平不高.指出四川省玉米品种选育应根据生产需要选择适宜当地生态条件的区域品种,继续加大高产、优质玉米新品种选育的投入力度.     

城市广场发展中的隐喻演变研究

通过对广场历史发展的研究,分析了4个广场的案例,得出了隐喻的5个不同时期的演变:民主政治的隐喻、帝王君权的隐喻、宗教神权的隐喻、人文精神的隐喻、历史文脉的隐喻.认为历史延续性和社会适应性是城市广场隐喻演变的两大特性.