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黑穗醋栗品种亲缘关系的ISSR分析
引用本文:宋杨,张春雨,张志东,温景辉,李亚东,吴林,刘海广.黑穗醋栗品种亲缘关系的ISSR分析[J].园艺学报,2011,38(9):1747-1752.
作者姓名:宋杨  张春雨  张志东  温景辉  李亚东  吴林  刘海广
作者单位:1. 吉林农业大学园艺学院,长春,130118
2. 吉林大学植物科学学院,长春,130062
3. 吉林省农业科学院,长春,130033
基金项目:农业部公益性行业(农业)科研专项(201103037,200903008-07-07); 吉林省科技发展计划项目(20080713)
摘    要: 采用ISSR标记技术对39个黑穗醋栗品种的亲缘关系进行分析。结果表明:13条ISSR引物扩增的总带数(A)从3到11条不等,平均扩增出6.77条带,平均多态性带百分比(P)为64.37%。聚类分析显示,在相似性系数为0.8496处将39个黑穗醋栗品种分为5组。第Ⅰ组包含8个起源国家的品种,说明世界各国之间基因交流较为频繁。‘Ben Lomond’和‘Kantata’同其他品种的遗传距离较远,两者又有诸多优良农艺性状,可作为黑穗醋栗的优良育种材料。

关 键 词:黑穗醋栗  ISSR  品种鉴定  亲缘关系
收稿时间:2011-6-9
修稿时间:2011-8-17

Genetic Relationship of Blackcurrant Cultivars Revealed by ISSR Markers
SONG Yang,ZHANG Chun-yu,ZHANG Zhi-dong,WEN Jing-hui,LI Ya-dong,WU Lin, LIU Hai-guang.Genetic Relationship of Blackcurrant Cultivars Revealed by ISSR Markers[J].Acta Horticulturae Sinica,2011,38(9):1747-1752.
Authors:SONG Yang  ZHANG Chun-yu  ZHANG Zhi-dong    WEN Jing-hui  LI Ya-dong  WU Lin  LIU Hai-guang
Institution:SONG Yang~1,ZHANG Chun-yu~2,ZHANG Zhi-dong~1,*,WEN Jing-hui~3,LI Ya-dong~1,WU Lin~1,and LIU Hai-guang~1(1College of Horticulture,Jilin Agricultural University,Changchun 130118,China,2College of Plant Science,Jilin University,Changchun 130062,3Jilin Academy of Agricultural Sciences,Changchun 130033,China)
Abstract:he genetic relationship of 39 blackcurrant cultivars was analyzed by inter-simple sequence repeats(ISSR). The results showed that the total number of bands(A)amplified by 13 ISSR primers ranged from 3 to 11,the average was 6.77,the mean percentage of polymorphic band(P)was 64.37%. Cluster analysis showed that 39 blackcurrant cultivars were divided into 5 groups at 0.8496 level of similarity coefficient. Cultivars in the groupⅠcame from 8 origin countries. It indicated that there was larger gene flow between countries.‘Ben Lomond’and‘Kantata’were farther with other blackcurrant cultivars in genetic distances. Both cultivars have high agronomic and fruit quality traits.‘Ben Lomond’and‘Kantata’will be good cultivars in the future breeding programme.
Keywords:blackcurrant  ISSR  cultivars identification  genetic relationship  
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