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91.
Characterization of plant-derived water extractable organic matter by multiple spectroscopic techniques 总被引:1,自引:0,他引:1
Zhongqi He Jingdong Mao C. Wayne Honeycutt Tsutomu Ohno James F. Hunt Barbara J. Cade-Menun 《Biology and Fertility of Soils》2009,45(6):609-616
Water extractable organic matter (WEOM) derived from fresh- or early-stage decomposing soil amendment materials may play an
important role in the process of organic matter accumulation. In this study, eight WEOM samples extracted with a 40:1 (v/w) water to sample ratio from alfalfa (Medicago sativa L.), corn (Zea mays L.), crimson clover (Trifolium incarnatum L.), hairy vetch (Vicia villosa L.), lupin (Lupinus albus L.), soybean (Glycine max L. Merr.), wheat (Triticum aestivum L.), and dairy manure were investigated using ultraviolet (UV)–visible, Fourier transform infrared (FT-IR), solution 31P nuclear magnetic resonance (NMR), and solid state 13C NMR spectroscopies. UV–visible and FT-IR spectra of the plant-derived WEOM samples were typical for natural organic matter,
but possessed less humic-like characteristics than dairy manure-derived WEOM. Solution 31P NMR spectra indicated that WEOM samples extracted from alfalfa, corn, and soybean shoots contained both orthophosphate and
monoester P. Of the monoester P in WEOM from soybean shoot, 70% was phytate P. WEOM from crimson clover, hairy vetch, lupin,
and wheat shoots contained orthophosphate only. The solid-state 13C NMR spectra of the seven plant-derived WEOM samples indicated that they all were primarily composed of sugars, amino acids
or peptides, and low molecular mass carboxylic acids. Carbohydrates were dominant components with very few aromatics present
in these samples. In addition, WEOM from crimson clover and lupin, but not other three leguminous plant WEOM samples, contained
significant asparagine. On the other hand, WEOM from corn and wheat contained less amino acids or peptides. The spectra of
WEOM of dairy manure revealed the presence of significant amounts of nonprotonated carbons and lignin residues, suggesting
humification of the manure-derived WEOM. Significant carbohydrates as well as aromatics were present in this WEOM. The P and
C bonding information for these WEOM samples may be useful for understanding the effects of WEOM on soil nutrient availability
to plants.
Trade or manufacturers' names mentioned in the paper are for information only and do not constitute endorsement, recommendation,
or exclusion by the USDA-ARS. 相似文献
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一种改进的双色SDS-PAGE凝胶和可视化上样电泳方法 总被引:1,自引:0,他引:1
SDS-PAGE电泳是蛋白质分析和研究中的重要高效手段.由于分离胶、浓缩胶与电泳缓冲液以及空气都是无色透明的物质,在胶的制作和点样过程中仅仅通过浓缩胶相与缓冲液水相或空气相对光的折射来准确判断点样孔的位置并进行点样操作,因而比较困难.该文介绍1种在浓缩胶中添加颜色染料,使浓缩胶显现颜色,以此可视化区分浓缩胶中点样孔(井)位置的方法,可以准确而高效完成点样操作,使传统的SDS-PAGE点样过程通过折光性来判断变为可视化准确判断,提高点样效率和电泳质量. 相似文献
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A new spectrophotometric assay for the quantification of polyphenolic content has been validated. It is based on Prussian Blue method and adapted to microplate spectrophotometry. Prussian Blue reaction is critically dependent on reaction time, but microplate spectrophotometry permits exact measures of many samples at the same time. This new method is precise, reproducible, repeatable and exact. 相似文献
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[目的]研究用荧光分光光度法测定水中苯酚和对苯二酚的含量,为环境样品中痕量物质的分析提供测定方法。[方法]取水样,加入β-环糊精溶液、EDTA溶液及缓冲溶液后测定。[结果]在波长对为λEx/λEm=240 nm/309 nm时测定苯酚含量,线性范围为0~1×10-4mol/L,检出限为6.6×10-8mol/L;在波长对为λEx/λEm=310 nm/340 nm时测定对苯二酚含量,线性范围为0~1.5×10-5mol/L,检出限为5.2×10-9mol/L。[结论]通过三维荧光扫描来选择干扰少而测定灵敏度较高的检测波长,实现了荧光分光光度法直接测定水样中的苯酚和对苯二酚含量。 相似文献
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分光光度法测定凤仙花中总黄酮的含量 总被引:1,自引:0,他引:1
[目的]建立能够准确测定凤仙花中总黄酮含量的方法。[方法]分别以芦丁、山柰酚为对照品,可见分光光度法是采用AlCl3显色,在412 nm波长处测定总黄酮的含量;紫外分光光度法在270 nm处测定总黄酮的含量。[结果]以芦丁、山柰酚为对照品采用可见分光光度法测得总黄酮含量的平均回收率分别为98.52%、99.72%,RSD分别为2.38%、3.14%;紫外分光光度法测得总黄酮含量的平均回收率分别为97.53%、98.75%,RSD分别为2.71%、2.86%。[结论]以山柰酚为对照品、采用AlCl3为显色剂的可见分光光度法是一种较理想的测定凤仙花中总黄酮含量的方法。 相似文献
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