基于三维荧光光谱法的水样中苯酚和对苯二酚含量测定

目的]研究用荧光分光光度法测定水中苯酚和对苯二酚的含量,为环境样品中痕量物质的分析提供测定方法。方法]取水样,加入β-环糊精溶液、EDTA溶液及缓冲溶液后测定。结果]在波长对为λEx/λEm=240 nm/309 nm时测定苯酚含量,线性范围为0～1×10-4mol/L,检出限为6.6×10-8mol/L;在波长对为λEx/λEm=310 nm/340 nm时测定对苯二酚含量,线性范围为0～1.5×10-5mol/L,检出限为5.2×10-9mol/L。结论]通过三维荧光扫描来选择干扰少而测定灵敏度较高的检测波长,实现了荧光分光光度法直接测定水样中的苯酚和对苯二酚含量。

Direct Determination of Phenol and Hydroquinone Contents in Environmental Water Based on Three-dimensional Fluorescence Spectroscopy

Objective] The research aimed to measure phenol and hydroquinone contents in water by fluorescence spectrophotometry,provide a new method to analyze trace substances in environmental samples.Method] The method firstly sampled water and added β-cyclodextrin and EDTA buffer solution in it,then the measurement was carried out.Result] Phenol were tested when wave length was at λEx /λEm=240 nm/309 nm.The results showed that the linear range was 0-1×10-4 mol/L and the detection limit was 6.6×10-8 mol/L.Hydroquinone was tested when wave length was λEx /λEm=310 nm/340 nm.The results showed that the linear range was 0-1.5×10-5 mol/L and the detection limit was 5.2×10-9 mol/L.Conclusion] The method of choose detection wave length with higher sensitivity and less interference by three-dimensional fluorescence scanning allows to determine directly the phenol and hydroquinone content by fluorescence spectrophotometry in water samples.