利用DNA分子标志鉴别台湾茶树品种及评估种原之遗传歧异性

为评估台湾茶树种原之遗传歧异性。本研究由100条ISSR引子申筛选出12条可产生多型性条带明显的引子．这些引子共可产生67个的多型性条带。依据每一种原之分子标志数据进行UPGMA法分群分析结果。可将台湾133个茶树种原区分成六大群，包括油茶群、赤芽山茶群、野生茶树群、大叶变种与小叶变种混合群、大叶、小叶及大叶、小叶杂交种混合群及小叶变种群。而主成分向量分析的结果与利用群聚分析得到的亲缘关系树形固结果相符合。台湾茶树种原高比例的遗传歧异度是由台湾的野生茶树所贡献．部分重要栽培种间的相似性仍极高。为了探讨制茶过程封分子级品种鉴定之影响及DNA分子标志应用于咸茶品种鉴定之可行性，本研究分析不同发酵程度的茶类。在制茶过程申封DNA质量之影响。试验结果显示高温杀菁过程严重造成咸茶DNA的降解。利用各种类别咸茶与新鲜茶叶（封照）所抽取之DNA样品进行PCR扩增反应．结果发现分子量小于1,000bp的ISSR DNA条带表现较稳定。     

绿茶原料低温保鲜技术研究

将微波杀青的茶鲜叶原料与鲜叶、干茶用有色塑料袋抽气包装,在不同低温温度下贮藏1年后烘干,在室温放置1个月,HPLC分析几种主要儿茶素含量,GC分析茶叶香气.检测结果表明,贮藏1年的杀青叶制成的绿茶在主要品质因子上均优于对照.因此,茶鲜叶微波杀青后,迅速低温去湿,在-5～-20℃下真空包装贮藏,可保鲜1年以上.     

Yield performance of interspecific F1 hybrids of the greek mountain tea, Sideritis spp. L.

Interspecific F₁ hybrids were produced after artificial intercrossing of six Sideritis species indigenous to Greece, namely: S. athoa, S. euboea, S. syriaca, S. raeseri, S. scardica, S. clandestina and a cultivated clone of S. raeseri. It was found that all species cross with each other, but the level of crossability varied with the cross-combination. In the yield evaluation experiments, the interspecific hybrids with a common female parent were considered as a group. It was observed that yield during the year of establishment was relatively low, but the interspecific hybrids out-yielded the control in both fresh and dry weight. All hybrids produced higher yields in the following years and their productivity started to decline after the fifth year. All hybrids produced higher yields than the control in all six years of the experiment. The highest yield over the years, in fresh and dry weight, was produced by the group Sideritis raeseri cultivated clone (cc) ×Sideritis spp. This yield, however, was not always significantly different from the yield produced by the second in the rank group (S. clandestina×Sideritis spp.). A high correlation (r > 0.99) between fresh and dry weight was observed. The two groups with the highest yield also had the highest essential oil content. In addition, all the interspecific hybrids had a higher essential oil content than the control species. Furthermore, the essential oil content of the selected individual hybrid plants ranged between the midparental value to a value higher than the high-parental species, indicating an additive and dominance gene action. It was also found that the content of the essential oil in the seven most important components (pinene, lemonene, cynene, menthone, copaene, caryophyllene and valerianic ester) varied from hybrid to hybrid. Given that Sideritis reproduces mainly asexually, the direct use of individual interspecific hybrids with higher yield and content in essential oil than the control species is possible in cultivation.     

六安瓜片绿色名茶可持续开发研究

历史名茶—六安瓜片被列为中国十大经典绿茶之一，是与其独特的生长环境、制作工艺和优良的品质分不开的。目前，六安瓜片开发在取得初步成就的同时，也存在着质量标准难以控制、生产成本偏高、品牌保护力度不够等问题。因此，必须改进六安瓜片的生产工艺，降低其生产成本，全面实施无公害茶目标，扶持龙头企业，打造知名特色品牌，才能确保六安瓜片绿色名茶的可持续发展。     

枸杞叶保健茶的研制

依据枸杞叶所含的营养成分和保健功能因子介绍了枸杞叶的保健作用.以枸杞叶为原料,开发研制具有保健作用的枸杞叶保健茶.探索了枸杞叶保健茶的加工工艺.     

茶业生物多样性与生态谴康

阐明了茶业生物多样性概念及其影响因子，生物多样性在维持茶业生态健康的作用，指出茶业生物多样性保持不仅仅在于保持茶园生态系统的完整性。更应注重茶业生态健康及其管理。     

优质茶鲜对品质成份在年周期中的动态变化

通过全年茶季各阶段测定,分析了芽叶的机械组成,叶、梗及整体的水浸出物、茶多酚、氨基酸、儿茶素总量和纤维素的动态变化。探明叶/梗比值可以作为鲜叶嫩度指标;而5个品质成份中,以水浸出物和氨基酸的季节性差异达极显著水平,并据此建立回归方程。     

黄瓜茉莉花茶保健饮料的研制

以黄瓜、茉莉花茶为主要原料,配以适量的白砂糖和柠檬酸,研制黄瓜茉莉花茶保健饮料。结果表明,黄瓜茉莉花茶保健饮料的最佳配方为:黄瓜汁20%,茉莉花茶汁40%,白砂糖10%,柠檬酸0.15%;稳定剂选用0.2%的海藻酸钠、耐酸性CMC-Na、黄原胶的混合液(1∶1∶1)对饮料的稳定效果最好。按此配方制作的饮料呈淡绿色,口感清爽,营养丰富,具有黄瓜和茉莉花茶混合的特殊滋味与香气。     

贵州茶树种质资源数据库的建立研究

通过对贵州现有茶树资源资料的收集、整理和发掘，从而建立贵州茶树种质资源数据库。本文对建立数据库的要求和方法进行了论述，并说明建立的重要和价值。     

天然抗氧化剂茶多酚在肉制品上的应用

茶多酚是一种新型的天然抗氧化剂,具有抗氧化能力强、使用安全、无副作用和防腐保鲜等优点。将茶多酚应用于肉制品,可以预防和减缓其腐败变质。目前茶多酚的应用虽然仍存在一些问题,但是它具有广阔的发展前景。