Iron uptake in a bicarbonate-resistant cell line of tobacco

Abstract

In alkaline soils, plant growth is impaired mainly by high pH and high concentration of bicarbonates. The bicarbonate concentration increases the pH value, and causes deficiency of iron. A bicarbonate-resistant cell line (BR line) of tobacco (Nicotiana tabacum L. cv. Burley21) was selected by adding excess bicarbonate ions (20 mmol L^?1) to the culture medium. The pH of the medium was buffered 8.0 to 8.3. Under these conditions, about 80% of iron in the medium became insoluble. However, under such conditions, the BR line grew well. In this report, we examined some characteristics of the growth and iron uptake in the BR line under iron-deficient (i.e. high pH or no-iron) condition. At pH 5.8, the Fe³⁺ reduction activity was not significantly different between the non-selected line and the BR line. At pH 8.0, however, the Fe³⁺ reduction activity of the BR line was higher than that of the non-selected line. In no-iron condition, the growth of the non-selected line was markedly reduced after 2 weeks, while the BR line was not affected. The content of malic acid in both lines increased with the medium pH, and the content in the BR line was higher than that in the non-selected line. The BR line was able to adapt to the conditions, which restricted iron uptake, such as high bicarbonate concentration, high pH, and low iron conditions. The high ability of Fe³⁺ reduction was maintained at even high pH conditions. Further, the BR line may be able to improve the utilization of iron in the cells.     

Relationship between Olsen P and Ammonium Lactate–Extractable P in Portuguese Acid Soils

The soil phosphorus (P) test in Portugal is based on extraction with ammonium lactate (AL) at acidic pH. Because this test is rarely used in other countries, it is desirable to see whether the AL-P values correlate with the more commonly used P tests. In this work, we compared AL-P with bicarbonate-extractable P (Olsen's method) for a group of 48 samples from Portuguese acidic soils differing widely in P status. Despite their contrasting composition, both reagents extracted phosphate from the same sources, albeit in different proportions. Both Olsen P and AL-P were strongly correlated with resin-extractable and dilute electrolyte-desorbable P, which are respectively related to the soil contents in phytoavailable P and P that can be released to runoff or drainage water. Olsen P and AL-P were strongly correlated (R² = 0.870), the correlation became stronger when three overfertilized soils were excluded (Olsen P = 2.35 + 0.45 AL-P; R² = 0.908; P ≤ 0.001, n = 45). No correlation was observed for a group of soils recently fertilized with Gafsa phosphate probably because the acidic AL reagent dissolved residual calcium phosphate, thus overestimating the soil content in desorbable P. On the basis of the present results and the AL-P-based fertility classes used for fertilizer recommendation purposes, Olsen P–based fertility classes were tentatively proposed for Portuguese acidic soils.     

Capercaillie (Tetrao urogallus) Lekking sites in fragmented Finnish forest landscape

The location of Capercaillie lekking sites was compared in forest mosaics of different level of fragmentation. In north Finland the stand characteristics of the lekking sites did not differ from those of randomly selected stands in the 1950s judged from forestry maps and stand registers. In the 1980s, when forestry had considerably altered stand structure, lekking stands were older, larger and had a higher timber volume than random stands. These features evenly changed from the lek centre to the distance of 4 km, to which distance the adjacent areas were assessed. Southern and central Finnish analyses, based on topographic maps, showed that the proportion of forest distinctly decreased with increasing distance from a lek centre, up to a distance of 1–1.5 km. The average patch size of lekking stands were largest in the north (where both cut areas and remaining stands are large) and smallest in central Finland dominated by fine‐grained forest landscape. The results are discussed in terms of landscape ecology and forestry planning.     

用最大熵法进行沥青路面工程实例的结构可靠性分析

结合工程实例以蒙特卡罗分析法为基本出发点,首先通过计算机根据弯沉和弯拉应力的功能函数来生成1000个功能函数的一个随机抽样,然后用大熵法拟合沥青路面的弯沉功能函数的密度函数,求出两种失效模式的失效概率。同时,把整个路段看作是一个由弯沉失效和弯拉应力失效的两种失效模式串联组成的网络系统,对整个系统进行了可靠性评估。     

原木截面形状扫描参数的计算

利用激光扫描技术得到原木截面的基本参数,通过计算得到比较精确的原木截面形状,在整根原木上进行周期扫描从而得到整根原木的形状参数。     

早熟苦瓜新品种春研2号的选育

春研2号苦瓜是以强雌系A94为母本、自交系B8为父本育成的早熟苦瓜一代杂种,表现早熟、丰产、连续结果能力强、抗逆性强、品质优等优良特性。果实青白色,棒形,单果约重400g,粒瘤,富有光泽,苦味适中,口感脆嫩,外观商品性及风味品质佳。可露地早熟栽培或春季保护地栽培,一般产量60000～90000kg/hm2,前期产量、总产量、品质性状优于蓝山苦瓜。     

地下水-土系统中苯并三唑去除方法研究

含苯并三唑的地下水很难用常规的水污染处理方法去除,因此迫切需要探讨它在地下水-土系统中有效的去除方法。详细介绍了吸附法、光激发氧化法和生物法,并且针对这些方法应用到地下水中的可行性进行了分析。结果表明,吸附法受到环境因素的限制,并会发生缓慢的解析过程,需进一步探索和完善;光激发氧化法去除效果较好但仍处于探索阶段,需改善耗费较多人力、物力、财力的缺点。目前的生物法很难对苯并三唑进行降解,尚需要进一步的研究。     

西瓜新品种爱能5 号的选育

爱能5 号母本系湘蜜西瓜F₁ 自交12 代得到的稳定单系XM12，父本系西农8 号F1 自交10代得到的稳定单系XL10。植株生长势强，分枝力强，易坐果。果实椭圆形，果皮黑色，单瓜质量4 ～7kg，果皮厚1.1 cm 左右，瓤红色，肉质细脆，汁多味甜，中心可溶性固形物含量10% 左右。全生育期93d（天）左右，中熟，雌花开放～果实成熟约需32 d（天）。高抗枯萎病，抗疫病和炭疽病，适宜在湖南省及其周边地区种植。     

生鲜乳中添加皮革水解蛋白的测定方法研究

L-羟脯氨酸是皮革水解蛋白构成的特征氨基酸,因此鉴定L-羟脯氨酸就是鉴定皮革水解蛋白。建立UV法测定生鲜乳中皮革水解蛋白,L-羟脯氨酸浓度在0μg/mL～2.0μg/mL范围呈良好的线性关系,R=0.9998。L-羟脯氨酸平均回收率为100.2%,猪皮粉平均回收率为98.1%,皮革粉平均回收率为99.9%。该方法回收率高,检出限低,工作曲线稳定,重复性好。     

基于项目的模拟电子技术课程教学模式研究

以《模拟电子技术》课程为对象,对基于项目的教学模式进行了探讨,主要提出了项目法教学的研究内容及需要解决的关键问题,并给出了具体的实施办法。