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101.
汴粉19号是以优良自育自交系ks305为母本,以自交系ks325为父本配制而成的番茄一代杂种。早熟,无限生长类型,植株生长势强,连续坐果能力强;成熟果粉红色,扁圆形,果面光滑,平均单果质量174g左右;果实硬度高,耐贮运。果肉质沙,品质佳。含有Ty1、Ty3a抗病基因,抗番茄黄化曲叶病毒病(TYLCV);田间对叶霉病、枯萎病和根结线虫病的抗性强于对照金棚8号和洛番9号。春秋保护地栽培每667m~2产量5800kg左右。适宜河南、河北、陕西、山东等地春秋保护地及露地种植。 相似文献
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103.
为阐明青花菜(Brassica oleracea var.italica)开花促进因子AGL19与开花整合子AGL24和SOC1蛋白的互作机制,从青花菜中克隆了AGL19、SOC1及AGL24基因。它们分别编码221、221和214个氨基酸,均为MIKC型蛋白,并且与甘蓝、油菜、大白菜等亲缘关系较近,AGL19与SOC1同属TM3/SOC1亚家族成员。酵母双杂交表明:AGL19与AGL24蛋白能互作,激活酵母报告基因AUR1-C、HIS3、ADE2和MEL1,在QDO/X-α-Gal/Ab A平板培养基上长出蓝斑;但与SOC1蛋白不能相互作用,说明AGL19的直接靶蛋白是AGL24而非SOC1。此外,青花菜SOC1也能与AGL24蛋白互作,说明AGL19可以通过AGL24间接与SOC1相互作用。 相似文献
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105.
为评价SARS-CoV-2特异性IgM和IgG抗体检测在2019-冠状病毒病(corona virus disease 2019, COVID-19)中的临床应用价值.本试验采用回顾性研究方法,选择重庆市公共卫生医疗救治中心2020年2月4-24日门诊和住院患者作为研究对象,其中COVID-19患者101例,排除COVID-19的其他肺部疾病及发热患者54例作为对照组,以RT-PCR检测结果作为金标准,采用胶体金免疫层析法(gold immunochromatography assay, GICA)检测所有研究对象的SARS-CoV-2特异性IgM和IgG抗体,使用诊断特异性、敏感度、诊断准确度等指标对诊断价值进行评价,并分析抗体与疾病病程以及疾病分型的关系,采用χ~2检验对检测结果进行统计学分析.结果表明, SARS-CoV-2特异性IgM/IgG临床诊断敏感度、特异度和诊断准确度分别为92.1%, 90.7%和91.6%,与RT-PCR方法相比,经过χ~2检验,二者差异无统计学意义(p0.05). SARS-CoV-2特异性IgM和IgG抗体阳性率与病程的关系经过χ~2检验,不同发病天数各组间差异有统计学意义(p0.05).不同疾病分型的SARS-CoV-2特异性IgM和IgG抗体阳性率经过χ~2检验,差异无统计学意义(p0.05).总之, SARS-CoV-2特异性IgG/gM抗体的检测可用于COVID-19感染的诊断,但在疾病早期可能存在漏检的风险. 相似文献
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107.
Yasuhiro UNO Hiroshi YAMAZAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):147-148
In cynomolgus macaques, which are widely used in drug metabolism studies, CYP2C19
(formerly known as CYP2C75) is abundantly expressed in liver, metabolizes human CYP2C
substrates and is thus an important drug-metabolizing enzyme. One of the cynomolgus
CYP2C19 alleles (p.Phe100Asn, p.Ala103Val and p.Ile112Leu) results in
substantially reduced metabolic activity and thus is an important allele in drug
metabolism studies. For this allele, a genotyping tool was developed using allele-specific
TaqMan probe. Genotyping 40 Cambodian cynomolgus macaques using this tool found 1
homozygote, 17 heterozygotes and 22 wild type animals, and the result was confirmed by
direct-sequencing. Interestingly, this allele frequency was similar to that of Chinese
cynomolgus macaques. The genotyping tool established is useful for drug metabolism studies
using cynomolgus macaques. 相似文献
108.
Jennie N. Jeyapalan Victoria. James David S. Gardner Jennifer H. Lothion-Roy Nigel P. Mongan Catrin Sian Rutland 《Anatomia, histologia, embryologia》2023,52(1):93-100
Research is often an essential component of completing a veterinary medicine degree, with universities worldwide aiming to teach students a variety of techniques and general research comprehension and skills. As universities worldwide navigated the COVID-19 pandemic, it was often necessary to move towards distance learning, this was employed for the research module at The University of Nottingham, School of Veterinary Medicine and Science. Following completion of their independent research project, each student cohort was sent a student evaluation of the module questionnaire and quantitative and qualitative analysis was undertaken. In addition, assessment outcomes based on dissertation grade, supervisor grade and overall module score were analysed quantitatively. This was conducted on both the individual cohorts and between the pre- and peri-pandemic groups, ranging from 2017–2018 through to 2021–2022 cohorts. The students received increased dissertation and supervisor grades (by nearly 6%) during the 2021–2022 peri-pandemic cohort, when compared to the pre-pandemic cohorts, but did differ significantly compared to the 2020–2021 cohort. The pre- and peri-pandemic Likert-scale ratings for module organisation and assessment criteria were similar, workload management and the ability to explore concepts and ideas was reduced in the peri-pandemic cohorts, whereas the accessibility to resources was increased in the peri-pandemic students compared to those taught prior to the pandemic. Student feedback can provide essential information when designing and managing research projects and when compared to assessment grades it can help us understand attainment, essential information when providing a quality university level education whilst supporting student welfare following the COVID-19 pandemic. 相似文献
109.
110.
从豆天蛾幼虫虫体中分离纯化出一种新型的豆天蛾核型多角体病毒(CbNPV),提取基因组DNA,进行基因组测序。结果发现一个与杆状病毒F蛋白基因同源性很高的读码框序列,该长度为2 109 bp,编码702个氨基酸。氨基酸序列同源性分析表明:CbNPV F蛋白与II类NPV和I类NPV F蛋白的同源性分别为32.9%~61.8%和8.0%~16.1%,并且将它与多种I类NPV杆状病毒同功能蛋白GP64进行分析,同源性在9.1%~11.4%,表明CbNPV F蛋白与I类NPV的F及GP64蛋白的同源性很低,因此认为CbNPV属于II类NPV。 相似文献