马铃薯蕾花果脱落与内源激素和光照的关系

以乌Ｈ_４比的母本０８１和父本ＮＳ４０－３７为材料,通过田间和室内试验,对马铃薯蕾花果脱落过程中内源激素变化进行研究,阐明了内源激素的变化规律及ＩＡＡ、ＧＡ_３、ＡＢＡ、Ｚ、ＺＲ对蕾花果脱落的具体作用方式;同时研究了不同光周期和光照强度与脱落的关系及所引起的内源激素的变化。结果表明,蕾花果中ＡＢＡ含量升高是引起脱落的主要原因,不利的外界因素可诱发ＡＢＡ的变化,而长光周期和充足的光照有助于减少蕾、花、果的脱落。     

茶树新梢生育的内源激素水平及其调控机理（第二报）茶树休眠与内源激素的关

本文研究了不同茶树品种新梢夏季生长休止、冬季休眠与内源激素水平的关系。结果表明,内源激素水平差异及激素间的比值变化与新梢生长和休止密切相关,高含量IAA及高IAA／zeatin与IAA／GA3比值,导致夏季生长休止;高含量ABA及高BAB／GA3与ABA／zeatin比值,是诱发冬季休眠的主要原因。     

Effect of Chinese Rice Wine on the Endogenous Protease Activity,Myofibrillar Degradation,and Quality Characteristics in Topmouth Culter (Culter alburnus)

ABSTRACT

The aim of this study was to investigate the influence of rice wine (Jinbiao, Yinbiao, Wunianchen, Nv’erhong) on the endogenous protease activity, myofibrillar degradation, and quality characteristics of topmouth culter (Culter alburnus). Rice wine had a pH of 4.3 and calcium ions ranging from 89.30 to 123.70 μg/mL. Nv’erhong rice wine had the highest total phenolic content of 583.38 μg gallic acid equivalent (GAE)/mL, while Wunianchen rice wine (WNC) showed the strongest total antioxidant capacity of 87.29 U/mL. WNC presented the optimum inhibitory effect on endogenous proteases of calpain and cathepsin B, D, and L, delaying the degradation of myosin heavy chain and α-actinin, which are important to muscle softening. The quality characteristic evaluation indicated that the addition of rice wine could maintain the hardness and chewiness, increase the springiness of the fish fillet, as well as inhibit the total volatile basic nitrogen production. These results suggest that rice wine had effects of inhibiting endogenous proteases, delaying myofibrillar degradation, and maintaining the sensory quality of topmouth culter.     

甜菜对豆磺隆的敏感性与内源激素关系的研究

文章采用水培试验方法,在豆磺隆4μg.kg-1条件下,对耐性及感性甜菜内源激素含量的动态变化进行测定。结果表明,耐性甜菜在豆磺隆施用后第3天IAA含量开始增加并高于对照7.9%,GA及Zr与敏感甜菜相比保持较高含量,ABA含量略有降低,而敏感甜菜ABA含量明显增加。耐性甜菜品系三种促进型激素IAA、GA及Zr与ABA比值较高,因而维持了耐性甜菜体内激素的相对平衡,在增强对豆磺隆耐性上具有重要作用。     

月季切花内源激素含量ELISA测定方法的建立

ELISA法测定植物内源激素含量具有专一、快捷等特点。介绍了ELISA法测定月季切花内源激素含量的具体步骤,建立了测定月季切花内源激素含量的ELISA方法。     

板栗子房内源激素含量与空苞形成关系的研究

采艇控制授粉方法分析了板栗授粉前后子房内源激素含量的变化。结果表明,授粉刺激了板栗子房内源ＧＡ１／３、ｉＰＡ８含量及（ＧＡ１／３＋ｉＰＡｓ）／ＡＢＡ值增加,并降低了子房内源ＡＢＡ含量。未授粉子房和正常授粉子房在授粉前后内源激素含量及变化趋势上的差异,与空苞栗子房和正常栗子房间同一时期的差异基本相似,且未授粉子房内激激素含量变化与空苞栗一致,因此可认为板栗子房授粉不良是空苞形成的重要原因。     

内源及外源植物激素对陆地棉无絮突变体胚珠纤维初始发育诱导效应的研究(英文)

采用陆地棉徐州１４２无絮突变体（ＦＬ）与其正常品种（ＷＴ）比较,研究了外源激素对离体胚珠纤维的诱导作用以及纤维初始发育过程中胚珠内源激素（ＩＡＡ,ＧＡ,ＡＢＡ和ｉＰＡ）含量的变化。结果表明,ＧＡ能够诱导离体的正常和突变体未受精和受精胚珠产生出纤维,开花前２ｄ～３ｄ的胚珠培养到天然开花日之后纤维方才突起。开花当天内源ＩＡＡ含量的急剧上升可能是胚珠表皮细胞迅速伸长的一个重要原因。开花前４ｄ到开花后４ｄ突变体胚珠内高水平的ｉＰＡ阻碍其纤维发生。     

海藻提取物缓解高温对樱桃萝卜胁迫效应的研究

选取樱桃萝卜(Raphanus sativus L.var.radculus pers)为研究对象,在盆栽试验条件下以清水处理为对照(CK),并以浓度为66.7 mg·L-1的芸苔素内酯为阳性对照(BHG),通过添加不同浓度(100.0、200.0 mg·L-1)的海藻提取物处理(SWE-1、SWE-2),探究了在高温...     

内源酶辅助提取虾壳虾青素的研究

文章探讨了内源酶对虾青素提取效果的影响,并以高效液相色谱法（HPLC）测定样品中虾青素的质量浓度,研究了pH、温度和酶解时间这3个因素对虾青素提取效果的影响,采用正交试验进行优化,以虾壳虾青素的萃取率作为评价提取效果的指标。结果表明,当酶解最佳工艺条件为pH 4.0,温度50℃,酶解时间1.5 h时,虾青素的萃取率最高,可达32.16μg.g-1湿虾壳,比直接超声提取提高28%。通过空白试验可知,外加脂肪酶对虾青素提取无促进作用,仅利用内源酶即可达到较好的提取效果,且经济节约。     

Inhibition of endogenous peroxidase activity in soil samples. Application to the analysis of soil‐bound triazine residues by enzyme immunoassays with peroxidase tracer

In the analysis of soil samples with immunochemical methods, the interpretation of tracer signals derived from marker enzymes such as peroxidase may be limited by the presence of endogenous enzyme activity. Therefore various inhibitors of peroxidase were tested for their ability to remove endogenous enzyme activity. Hydrogen peroxide, sodium azide and phenylhydrazine effectively inhibited a commercial peroxidase, while only phenylhydrazine proved to be a successful inhibitor of peroxidase in soil samples. An optimized enzyme immunoassay was then used for the investigation of soil samples for non‐extractable atrazine residues. Only soil samples where atrazine had been applied during preceding years yielded positive signals in contrast to a control soil that came from a barn with no atrazine applied.