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为筛选适合甜菜品种纯度鉴定的SRAP核心引物,以12个进口国外甜菜品种为材料,对546对SRAP引物组合进行扩增,利用8%的非变性聚丙烯酰胺凝胶电泳进行分离,选择适合甜菜品种纯度鉴定的SRAP核心引物组合。结果从546对SRAP引物组合中筛选出扩增条带清晰、多态性丰富、重复性好的引物组合23对,这23对引物组合共扩增出177条多态性条带,引物的PIC值均大于0.6。平均每对引物扩增7.7条多态性条带,从理论上讲,这些核心引物完全可以实现对现有甜菜品种的鉴定。 相似文献
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J. E. Parlevliet 《Euphytica》2007,153(3):353-362
Improved cultivars loose their identity and healthiness unless maintained properly. Contaminating and degrading forces, such
as outcrossing, volunteer plants, mixing, natural selection, mutation and seed-borne diseases, are at the root of this. Maintenance
selection can prevent this deterioration. How it is carried out depends on the reproduction system of the crop. Crops are
therefore classified into four categories; typical cross-pollinating crops, self-pollinating crops with a substantial amount
of outcrossing, typical self-pollinating crops with little outcrossing, and the vegetatively reproduced crops.
Generally some of the “breeder seed” is used to plant a small plot with spaced plants. A fair number of healthy plants of
the cultivar type is selected and the seed is harvested per plant. The progenies of the selected plants are grown in small
plots. Non-uniform or deviating plots and plots with a seed-borne disease are removed. The seed of the progenies that are
healthy, uniform and similar (and so of the cultivar type) are harvested per progeny to be tested next season on larger plots.
The same selection is applied and only the seed of the progenies that are healthy, uniform and similar are harvested together
to produce the “breeder seed”. The details of this maintenance selection vary with the reproduction system, the multiplication
rate of the crop and the possibilities available to the breeder. Seven crops, potato, common bean, barley, wheat, faba bean,
quinoa and maize are discussed here as they represent the different reproduction systems and multiplication rates, while being
important Andean food crops. 相似文献
90.
Summary Isozyme analyses have been used for the definitive identification of many plant cultivars, but not for cultivated tomatoes. Six isozyme systems, namely alcohol dehydrogenase, acid phosphatase, phosphoglucomutase, esterase, phosphoglucoisomerase and 6-phosphogluconate dehydrogenase of tomato seed extracts were resolved by isoelectric focusing on polyacrylamide gels with a narrow pH gradient. Nine alcohol dehydrogenase phenotypes were distinguished which, with three acid phosphatase phenotypes, identified twelve of the seventeen cultivars. Fewer differences were found for the other isozymes. Since this method could differentiate between breeding parents and their progeny it is concluded that further investigations are warranted.Abbreviations APS
acid phosphatase
- ADH
alcohol dehydrogenase
- EST
esterase
- IEF
isoelectric focusing
- PGI
phosphoglucoisomerase
- PGM
phosphoglucomutase
- 6-PGDH
6-phosphogluconate dehydrogenase
- RFLP
restriction fragment length polymorphism
- VOPRI
Vegetable and Ornamental Plant Research Institute 相似文献