全文获取类型
收费全文 | 2644篇 |
免费 | 78篇 |
国内免费 | 217篇 |
专业分类
林业 | 119篇 |
农学 | 169篇 |
基础科学 | 20篇 |
160篇 | |
综合类 | 1378篇 |
农作物 | 215篇 |
水产渔业 | 125篇 |
畜牧兽医 | 444篇 |
园艺 | 70篇 |
植物保护 | 239篇 |
出版年
2024年 | 6篇 |
2023年 | 14篇 |
2022年 | 53篇 |
2021年 | 70篇 |
2020年 | 71篇 |
2019年 | 76篇 |
2018年 | 49篇 |
2017年 | 116篇 |
2016年 | 129篇 |
2015年 | 141篇 |
2014年 | 171篇 |
2013年 | 168篇 |
2012年 | 248篇 |
2011年 | 253篇 |
2010年 | 180篇 |
2009年 | 175篇 |
2008年 | 146篇 |
2007年 | 162篇 |
2006年 | 117篇 |
2005年 | 95篇 |
2004年 | 60篇 |
2003年 | 53篇 |
2002年 | 34篇 |
2001年 | 37篇 |
2000年 | 42篇 |
1999年 | 26篇 |
1998年 | 34篇 |
1997年 | 40篇 |
1996年 | 26篇 |
1995年 | 18篇 |
1994年 | 15篇 |
1993年 | 13篇 |
1992年 | 15篇 |
1991年 | 14篇 |
1990年 | 13篇 |
1989年 | 14篇 |
1988年 | 12篇 |
1987年 | 7篇 |
1986年 | 2篇 |
1985年 | 7篇 |
1984年 | 6篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有2939条查询结果,搜索用时 31 毫秒
41.
42.
43.
研究了用3种大口径毛细管柱分析由有机磷杀虫剂组成的“难分离有机磷对”。HP-1大口径毛细管柱只能分离由甲基对硫磷和其它有机磷杀虫剂组成的“有机磷对”;QF-1大口径毛细管柱能够分离部分“有机磷对”;阿皮松L固定液大口径毛细管柱能完全分离马拉硫磷和水胺硫磷组成的“有机磷对”。 相似文献
44.
45.
C. Caprari C. Bergmann Q. Migheli G. Salvi P. Albersheim A. Darvill F. Cervone G. De Lorenzo 《Physiological and Molecular Plant Pathology》1993,43(6)
Extracellular endopolygalacturonase, purified from the pathogenic fungus Fusarium moniliforme, consists of four molecular forms (38, 41·5, 45, and 48·5 kDa, respectively. Three forms (38, 41·5, and 45 kDa) were purified to homogencity by FPLC on a Mono S column followed by electroelution after SDS-PAGE. The N-terminal amino acid sequences of each of the three forms, and of a mixture containing all four forms were shown to be identical to that predicted from the nucleotide sequence of the endopolygalacturonase gene previously cloned from F. moniliforme. Enzymatic deglycosylation experiments revealed the presence of N-linked, high mannose oligosaccharide side-chains on all four forms of endo polygalacturonase. Hydrogen fluoride catalysed chemical deglycosylation of the polygalacturonase mixture yielded a single polypeptide with an apparent molecular mass of 36·2 kDa. Southern blot analysis, carried out at high stringency with an endopolygalacturonase-specific probe on genomic DNA digested with three different restriction enzymes, showed a single hybridizing restriction fragment in all three digests. A single 2·0 Mb chromosome hybridized with the endo polygalacturonase-specific probe, as shown by Southern blot analysis of F. moniliforme chromosomes separated by CHEF electrophoresis. Northern blot analysis revealed only one mRNA species 1350 nt encoding endo polygalacturonase. These data indicate that a single gene encodes the endopolygalacturonases of F. moniliforme. 相似文献
46.
Capece BP Castells G Pérez F Arboix M Cristòfol C 《Veterinary research communications》2000,24(5):339-348
Benzimidazole anthelmintic drugs are widely used in veterinary practice. Albendazole sulphoxide (ABZSO) is a benzimidazole drug with two enantiomers, as a consequence of a chiral centre in the sulphoxide group. The kinetics of these enantiomers were studied in male and female sheep. Plasma samples were obtained from the animals between 0.5 and 72 h after oral administration of 7.5 mg/kg of a racemic formulation of ABZSO (total-ABZSO). After a liquid–liquid extraction, the samples were analysed by HPLC to determine the concentrations of total-ABZSO and of the sulphone metabolite (ABZSO2). During the chromatographic analysis, the ABZSO peak was collected and reanalysed by an HPLC technique using a Chiral AGP column to quantify the enantiomeric proportion therein. After kinetic analysis, the AUCs obtained for the (+)-ABZSO were 5.8 and 4.0 times higher than those for the (–)-ABZSO in male and female animals, respectively. The mean residence times were 23.4 and 16.1 h for (+)-ABZSO and 22.2 and 17.4 h for (–)-ABZSO for male and female animals, respectively. The only significant difference between the sexes (p<0.05) was in the T
max of the (–)-ABZSO. Comparing both enantiomers within each sex, significant differences were found in all the kinetic parameters. Finally, no kinetic differences were found between sex for total-ABZSO or ABZSO2. 相似文献
47.
本实验建立了饲料中有机氯农药(OCPs)残留的气相色谱-微池电子捕获检测器(GC-μECD)分析方法。试样经丙酮提取,硅胶层析柱净化后,用GC-μECD测定其中农药残留量。有机氯农药浓度在5.00~500.00ng/mLp,p’-DDD相关系数除为0.9960外,其余均大于0.998,线性关系良好;检测限除o,p’-DDT和p,p’-DDT为10μg/kg外,其余农药为5μg/kg;添加有机氯农药50μg/kg水平时回收率为77.1%~113.4%,添加200μg/kg时回收率为75.8%~107.3%;相对标准偏差为2.3%~11.1%。本方法对饲料中有机氯农药残留分析结果准确可靠。 相似文献
48.
采用高效液相色谱法(HPLC)建立了新型抗球虫中药复方TF-103的指纹图谱。实验采用Dia-monsil C18色谱柱(250mm×4.6mm5μm i.d.);流动相:A相为0.2%磷酸和0.1%三乙胺的水溶液,B相为乙腈,梯度洗脱;柱温:30℃;流速:1.0ml/min;紫外检测波长:330nm。以内标物香兰素、总峰面积以及峰面积最大的9个共有峰为评价指标,并采用“中药色谱图分析和数据管理系统”软件进行了相似度计算。结果表明,该法操作简单,精密度和稳定性良好,有助于该中药复方的质量控制和谱效关系的研究。 相似文献
49.
50.