反向遗传技术在牛轮状病毒研究中的应用

本文综述了牛轮状病毒的生物学特性、反向遗传学系统的建立及其研究进展,并对反向遗传技术应用于牛轮状病毒进行展望。     

我国马铃薯主产区病毒病发生情况调查

为了解我国马铃薯病毒病发生情况,在马铃薯主产区黑龙江、内蒙古、甘肃、云南等马铃薯田采集了具有典型病毒病症状的样品、疑似样品和随机无症样品,试管苗和原原种为随机样品,共649份。应用DASELISA方法筛查6种马铃薯主要病毒:PVX、PVY、PVS、PLRV、PVM和PVA。结果表明:129份样品为阳性,PVY的检出率最高,为9.86%,PVS次之,为6.47%;试管苗PVS检出最多,为32份,原原种PVX检出最多,为10份,大田样品PVY病毒发生比例最高,为54份;有38份样品是由多种病毒复合侵染造成的,大田PVY+PVS复合侵染率最高,为2.93%,PVS+PVY+PLRV次之,为0.98%,试管苗PVS+PVM复合侵染率最高,为4.85%。通过历年的检测结果比较,试管苗中PVS病毒检出率最高,原原种中PVX和PLRV病毒成为危害最为严重的病害,大田样品中PVY检出率一直居高不下。     

水质快速检测设备的硬软件设计及实现

[目的]研制本质快速检测设备,实现水质的现场多指标快速检测.[方法]对设备的硬件及软件进行了设计.硬件设计包含了1P2303芯片、锂电,Cortex-M3型ARM微控制器、树莓派显示系统、光频转换器等的设计;软件设计则包含了软件设置、项目选项、项目测量结果以及测量数据表格输出等模块的设计;并对水质快速检测设备的可靠性进行了试验检验.[结果]通过对该设计的设备硬件和软件合理搭配连接,从而研发出了水质快速检测设备.用该设备与国家标准要求的设备同时测定某一河道水样的5项指标结果发现,3个样点水样中铁、氨态氮、氟化物、硝酸盐和6价铬含量的两组数据值接近,无明显差异.虽然国家标准法检测下限比快测法高一个数量级,但快速设备的测定结果不影响操作员对水质的判断,说明该水质快速检测设备精密度高;两种测定方法的稳定性比较,结果快速测定法的5个指标数据的相对偏差略高于国家标准法(3.92％),但其测定结果能够较好地满足现场测定要求,说明该水质快速检测设备稳定性良好.[结论]该水质快速检测设备的检测精度和稳定性较高,可满足水质的现场快速检测要求.     

高校图书馆为企业技术创新提供信息服务调查分析

信息是企业进行技术创新的重要战略资源。本文从高校图书馆为企业提供信息服务的实践调查出发,总结出目前服务体系中存在的问题,梳理出提升企业技术创新能力的对策。     

水肥耦合对小麦/玉米带田产量及构成因素的影响

采用"3414"最优回归设计方案,于2007-2008年在甘肃省农业科学院张掖节水试验站,开展不同水肥耦合处理小麦/玉米带田产量效应研究。结果表明,不同水肥耦合模式对小麦/玉米带田产量的影响较大,其差异达极显著水平。其中,氮肥对产量的贡献最大,水分次之,磷肥最小,氮肥对带田混合产量的绝对贡献率达79.4%,而水分对带田混合产量的绝对贡献率达为52.9%;水肥耦合效应为:水氮耦合水磷耦合氮磷耦合;获得高产量12 952.5~13 880.0kg/hm2的施氮量为420~630kg/hm2、灌水量为5 550~6 750m3/hm2、施磷量为120kg/hm2。相关分析表明,施氮量和灌水量与间作玉米的穗粒数、千粒质量、穗粒质量、株高均呈极显著正相关,施氮量与间作小麦的穗粒质量、穗粒数呈极显著正相关。     

Design and performance of recirculating aquaculture system for marine finfish broodstock development

Tropical and subtropical climatic conditions in India present an ideal and unique opportunity for being the leader in tropical marine finfish aquaculture. However, the problem persist due to non-availability of marine finfish seed for the culture. In response to this problem, broodstock development of different tropical marine finfishes for seed production was started. The present study was undertaken to design a recirculating aquaculture system (RAS) and studying their performance in managing the various water quality required for the marine finfish broodstock development and breeding. The design of RAS, developed in the present study, included a broodstock tank, egg collection chamber, electrical pump, rapid sand filter, venturi type protein skimmer and biological filter. Two RAS were designed, one was stocked with a demersal fish species, orange spotted grouper (Epinephelus coioides) and the other was stocked with a pelagic fish species, Indian pompano (Trachinotus mookalee) at the rate 1 and 0.5 kg/m³ with a sex ratio of 1:1 and 1:2 (female: male) respectively. Various physio-chemical parameters, viz, total ammonia nitrogen (TAN), nitrite, nitrate, pH, alkalinity, temperature, free carbon dioxide (CO₂) and dissolved oxygen (DO) of both tank water were analyzed to assess the performance of recirculating aquaculture system in maintaining the water quality. Gonadal development of the fishes was assessed and the spawning performance was recorded and finally, economic performance of the system was also evaluated. During the entire experimental period, mean monthly total ammonia nitrogen was less than 0.07 and 0.06 mg L⁻¹ and mean monthly nitrite was less than 0.02 and 0.01 mg L⁻¹ in orange spotted grouper and Indian pompano RAS tanks respectively. The pH (7.8–8.2), DO (>4 mg/L) and alkalinity (100–120 mg/L) were found to be in optimum range in both recirculating aquaculture systems. Carbon dioxide was found to be nil during the entire experimental period in both the systems. In fact these levels were comparable or less than that is reported as the permissible limits for broodstock development. Indian pompano and Orange spotted grouper matured and spawning was obtained with production of fertilized eggs round the year. Economic evaluation showed the price of 10,000 fertilized eggs of orange spotted grouper to be US $ 1.33. The design of RAS devised in the present study is efficient in controlling and maintaining optimum water quality for broodstock development of both demersal and pelagic finfishes. The fishes stocked in RAS attained final maturation and round the year spawning was obtained.     

农业绿色高效节水领域研究现状与思考

节水农业是水资源高效利用和农业可持续发展的重大问题，对于节约水资源具有极其重要的意义。为了解当前绿色农业高效节水领域的最新研究热点和发展趋势，本文梳理总结了我国现代化节水农业领域的基础背景、研究现状以及发展态势。结果表明，“灌溉设备与技术开发”、“农业用水精量调配与控制”、“作物高效用水机理”及“农业用水对环境变化的响应”等是近年来的研究热点，并建议开展水肥一体化智能技术与管理及其对作物、土壤、环境、社会等综合效应等的相关研究，以期为我国农业绿色高质量发展提供帮助。     

第24届冬奥会举办地空气质量特征及环境因子响应机制

空气质量问题是近些年来广受关注的问题，尤其对国际性盛会冬奥会而言，举办地的空气质量也是其能否举办成功的重要评估标准。为评估冬奥会举办地近6 a空气质量状况及动态变化环境相应机理，该研究收集了2014年以来3个冬奥会场馆所在地（北京延庆、奥体中心和河北张家口）的空气质量数据，分别分析了3个监测点的6个空气质量指标（CO、NO₂、O₃、SO₂、PM_2.5、PM₁₀）的浓度动态，并结合2016年奥体中心的气象数据分析了6个指标环境因子的响应机制，结果表明:1)张家口空气质量相对最佳，PM_2.5达标率均在80%以上，但春季大颗粒物污染仍需治理；2)2014-2019年3个监测点的空气质量有明显改善，尤其张家口SO₂从37.50 μg/m³（2014）降至11.06 μg/m³（2019），但截至2019年奥体中心NO₂仍高于国际标准，气体污染物仍是其治理的重点；3)CO和NO²日变化呈“U型”，O³和SO²为“单峰型”，CO、O₃、SO₂季节变化明显。各空气质量指标的主要影响因子分别为CO-太阳净辐射（R_n）、NO₂-太阳净辐射（R_n）、O₃-空气温度（T_a）、SO₂-空气温度（T_a），其中风速（Ws）与各空气质量指标呈负相关关系；6个指标年最大值（7.6 mg/m³、155.5、186.9、68.4、373.2 μg/m³和465.5 μg/m³）均出现在Ws<1 m/s时；然而，由于各空气质量指标的来源及彼此转换，各空气质量指标之间的相关性强于与环境因子的相关性。     

我国楠属种质资源分布现状及主要种特征差异

对福建、江西、浙江、湖南、湖北、贵州、重庆、四川、海南、云南及广西11省(自治区、直辖市)70个县(区、市)的楠属(Phoebe)树种种质资源进行详细调查,记录楠属种质资源分布、群落现状、木材利用及人工造林情况,对种质资源进行收集保存,并详细介绍主要树种的特征差异。调查发现,中国楠属树种资源主要分布在长江流域及以南地区,其中紫楠(P.sheareri)、闽楠(P.bournei)及白楠(P.neurantha)分布范围最广;综合比较生长速度和适应性,广西适宜推广种植的楠属树种有闽楠、桢楠(P.zhennan)及崖楠(P.yaiensis)。     

Soil physical changes and maize growth in a structurally fragile tropical soil due to mulching and duration between irrigation intervals

Under tropical meteorological conditions, the volume of soil explored by plant roots is crucial for crop growth as it allows increased water and nutrient use efficiency. We hypothesized that, under different irrigation intervals, leguminous mulch can extend the duration between irrigation events but maintain crop performance, because decreased evaporative fluxes also reduce constraints to root exploration imposed by mechanical stress. We evaluated the combined effects of leguminous mulch and irrigation intervals on soil physical properties to determine whether the growth and productivity of maize were modified in a structurally fragile tropical soil. The experiment involved the following treatments: 4‐day irrigation intervals with soil mulched (4C) or bare (4S), 6‐day irrigation intervals with soil mulched (6C) or bare (6S), 8‐day irrigation intervals with soil mulched (8C) or bare (8S) and 10‐day irrigation intervals with soil mulched (10C) or bare (10S). Mulch decreased soil penetration resistance and increased to 4 days the favourable time for root development in drying soil. Relative to bare soil, mulch with a 6‐day irrigation interval almost doubled nitrogen uptake post‐tasselling, which decreased nitrogen remobilization and increased the crop growth rate during this stage. These conditions had a positive effect on the transpiration rate and stomatal conductance as well as on the growth and yield of maize. A 6‐day irrigation interval with mulch compared to 4 days with bare soil resulted in similar conditions for root development, but greater uptake of nitrogen (102.73–78.70 kg/ha) and better yield (6.2–5.3 t/ha), which means greater efficiency in nitrogen and water use.