我国马铃薯主产区病毒病发生情况调查

为了解我国马铃薯病毒病发生情况,在马铃薯主产区黑龙江、内蒙古、甘肃、云南等马铃薯田采集了具有典型病毒病症状的样品、疑似样品和随机无症样品,试管苗和原原种为随机样品,共649份。应用DASELISA方法筛查6种马铃薯主要病毒:PVX、PVY、PVS、PLRV、PVM和PVA。结果表明:129份样品为阳性,PVY的检出率最高,为9.86%,PVS次之,为6.47%;试管苗PVS检出最多,为32份,原原种PVX检出最多,为10份,大田样品PVY病毒发生比例最高,为54份;有38份样品是由多种病毒复合侵染造成的,大田PVY+PVS复合侵染率最高,为2.93%,PVS+PVY+PLRV次之,为0.98%,试管苗PVS+PVM复合侵染率最高,为4.85%。通过历年的检测结果比较,试管苗中PVS病毒检出率最高,原原种中PVX和PLRV病毒成为危害最为严重的病害,大田样品中PVY检出率一直居高不下。

Investigation on Virus Disease of the Main Potato Producing Areas in China

In order to investigate the occurrence of viruses on potato around China, 649 samples were collected in main potato producing areas including Heilongjiang, Inner Mongolia, Gansu and Yunnan province. Six viruses of PVX,PVY,PVS,PVM,PVA and PLRV were tested by DAS-ELISA. The results showed that there were 129 copies of the samples which were tested as positive,PVY had the highest detection rate which was 9.86% ,and PVS followed by 6.47% while PVS was the highest detection in the virus-free plantlets which was 32 samples, PVX was the highest in pre-dlitey,PVY was highest in field which was 54 samples;which was 10 samples. 38 samples were infected by infection with multiple viruses,in the field, compound infection rate was the highest of PVY＋PVS which was 2.93% ,and PVS＋PVY＋PLRV was 0. 98%. PVS＋PVM were compound infection rate in the virus-free plantlets which was 4.85 % ;Comparing the results of over the years,PVS was the highest detection rate in virus-free plantlets,PVX and PLRV become the most serious disease in preelite, the potao vi- rus ineidence of PVY was the highest in field.