狐阴道加德纳氏菌疫苗免疫狐血清抗体消长规律

应用ＤｏｔＥＬＩＳＡ检测狐阴道加德纳氏菌疫苗免疫狐的抗体水平及其哺乳仔狐的母源抗体水平。结果表明,接苗后４天开始产生抗体,７天阳转率７２７％,１０天时免疫狐１００％抗体阳转,２１～３０天抗体效价达高峰。接种后６个月血清抗体仍在１∶６４０以上,哺乳仔狐母源抗体随日龄增长而逐渐降低。３０日龄前,平均抗体效价（ＧＭＴ）为１∶２２６,以后逐渐降低。５０日龄抗体效价只有１∶１２。通过免疫抗体与攻毒保护相关性测试,抗体效价在１∶３２０以上,能抵抗１００个ＩＤ５０狐阴道加德纳氏菌强毒攻击。     

饲粮铬对0—3周龄肉鸡生长,血清生化特性和免疫功能的影响

用１日龄Ａｒｂｏｒ Ａｃｒｅｓ（ＡＡ）商品代肉公鸡３８４只,研究饲粮添加铬源与铬水平对００－３周龄肉鸡生长,血清生化特性和免疫功能的影响。试鸡按体重随机分成８组,分别喂以添加铬的玉米－豆粕型基础饲粮（含铬０．３４ｍｇ／ｋｇ）及这种基础饲粮分别添加０．４,２．０,１０．０ｍｇ／ｋｇ铬分别源于三氯化铬和酵母铬的饲粮以及２０．０ｍｇ／ｋｇ铬源于三氯化铬的饲粮。     

蟋蟀粉替代鱼粉对黄颡鱼幼鱼生长性能、肌肉氨基酸组成和血清生化指标的影响

为研究蟋蟀(Gryllus bimaculatus)粉替代鱼粉对黄颡鱼(Pelteobagrus fulvidraco)幼鱼生长性能、肌肉成分和血清生化指标的影响,以黄颡鱼幼鱼[(2.0±0.13) g]为研究对象,分别使用蟋蟀粉替代0 (对照组)、15%、30%、45%和60%的鱼粉配制成5组等氮等能的实验饲料,分别记为T0、T15、T30、T45和T60组。实验幼鱼在室内循环水系统进行为期10周的养殖实验。结果显示,随着蟋蟀粉替代量的增加,黄颡鱼幼鱼生长呈先增加后降低的趋势;T30组的终末均重(FBW)、增重率(WGR)和特定生长率(SGR)最高,且均显著高于T0组;T30组的饲料系数(FCR)显著低于T0和T15组;T30的肝体比(HSI)显著高于T0和T15,而与T45和T60组无显著差异。与T0组相比,各替代组的脏体比(VSI)、摄食量(FI)、肥满度(CF)和成活率(SR)均无显著差异。黄颡鱼幼鱼肌肉必需氨基酸(EAA)含量中,T60组肌肉中精氨酸(Arg)和缬氨酸(Val)含量显著高于T0组;且蟋蟀粉替代不同比例的鱼粉对黄颡鱼幼鱼肌肉中的总呈味氨基酸(TFAA)含量均未产生显著影响。T30、T45和T60组血清中的葡萄糖(GLU)含量显著高于T0组;相反,其总胆固醇(TCHO)含量显著低于T0组。在本研究条件下,蟋蟀粉替代不同比例的鱼粉不影响黄颡鱼幼鱼的生长性能和肌肉氨基酸含量,且能增加血清GLU和降低TCHO含量,以30%的蟋蟀粉替代鱼粉比例能生长最佳,且优于对照组。本研究对揭示蟋蟀粉在黄颡鱼饲料中替代鱼粉应用的可行性、为今后昆虫蛋白源在水产饲料中的开发提供科学依据。     

酶解磷虾粉替代鱼粉对珍珠龙胆石斑鱼幼鱼生长性能、体组成及血清生化的影响

以酶解磷虾粉分别替代基础饲料中0(T0)、10％(T10)、20％(T20)、30％(T30)、40％(T40)、50％(T50)的鱼粉,配制6种等氮等能的饲料,研究饲料中酶解磷虾粉替代鱼粉对珍珠龙胆石斑鱼幼鱼生长性能、体组成、血液生化指标、抗氧化性能及氟残留的影响.选取初始均重为(57.80±0.28)g的珍珠龙胆石斑幼鱼360尾,随机分为6组,每组3个重复,试验周期为56 d.结果显示,(1) T30组的增重率、特定生长率和摄食率均显著高于T0组(P＜0.05);在替代量大于20％时,饲料干物质表观消化率显著高于T0组()＜0.05);各替代组的饲料系数、肥满度、肝体比、蛋白质效率、蛋白质消化率与T0组无显著差异(P＞0.05).(2)各替代组全鱼和肌肉水分、粗脂肪、粗灰分含量与T0组无显著差异(P＞0.05);T30组肌肉粗蛋白质含量显著高于T0组(P＜0.05).(3)各组间血清谷丙转氨酶、谷草转氨酶、总蛋白、白蛋白、碱性磷酸酶、甘油三酯和高密度脂蛋白含量无显著差异(p0.05);T30组尿素氮含量显著低于T0组(P＜0.05);各替代组低密度脂蛋白含量显著高于T0组(P＜0.05).(4)酶解磷虾粉替代鱼粉显著升高了珍珠龙胆石斑鱼幼鱼血清和肝脏中谷胱甘肽过氧化物酶及血清超氧化物歧化酶的活力(P＜0.05),同时,显著降低了血清和肝脏中丙二醛含量(P＜0.05).(5)各组肌肉氟含量均在可检测范围以下,各组间骨骼氟含量随着替代比例的升高而升高,各组差异显著(P＜0.05).研究表明,综合考虑生长性能、体成分、生化指标及抗氧化性能,酶解磷虾粉可替代珍珠龙胆石斑鱼饲料中40％以下的鱼粉,替代量为30％时效果最佳.     

植物精油对广西灵山土鸡生长性能、养分消化率和血清抗体指标的影响

试验旨在研究饲粮中添加植物精油对广西灵山土鸡生长性能、养分表观消化率、血清抗体指标的影响.将1800只30日龄、体重(284.09±1.18)g、体况良好的广西灵山土鸡,随机分为3组,每组3个重复,每个重复200只鸡,空白对照组饲喂基础饲粮,混合植物精油组、抗生素组分别在基础饲粮中添加100 mg/kg混合植物精油、3...     

光照时间对就巢期浙东白鹅血清生理生化指标、盲肠挥发性脂肪酸和肠道菌群的影响

本试验旨在探究光照时间对就巢期浙东白鹅血清生理生化指标、盲肠挥发性脂肪酸和肠道微生物组成的影响.选取16只220日龄、体重相近且健康正常的浙东白鹅,随机分为2组,每组6个重复.长光照组和短光照组每日分别给予15 h(15L:9D)和9 h(9L:15D)的人工光照,饲养管理条件相同,试验期60 d.试验结束后采集鹅血清...     

蛋氨酸锌对40日龄吉戎兔血清生化指标的影响

随机选取40日龄健康吉绒Ⅱ系吉戎兔72只,分成4组,每组3个重复,每个重复6只。试验I为对照组,饲喂基础饲料;试验Ⅱ、Ⅲ、Ⅳ分别添加40,80,120mg/kg蛋氨酸锌。结果表明,日增重在试验期0～15d期间及16～30d期间,试验Ⅲ与其它组差异显著（P〈0．05）,分别比对照组、试验Ⅱ、Ⅳ组提高13．94％和11．31％、6．60％和5．93％及7．47％和8．44％。料重比在试验期0～15d期间,试验Ⅲ与其它组差异显著（P〈0．05）,分别比对照组、试验Ⅱ、Ⅳ组改善12．23％、6．19％及6．95％;在试验期16～30d期间,试验Ⅲ和试验Ⅱ差异不显著（P〉0．05）,但与对照组和试验Ⅳ差异显著（P〈0．05）,试验Ⅲ比对照组、试验Ⅱ、Ⅳ组改善10．16％、5．60％、7．78％。试验Ⅳ血清锌和白蛋白含量与对照组差异显著（P〈0．05）。试验组血清碱性磷酸酶、总蛋白含量与对照组差异显著（P〈0．05）,各处理组间尿素氮差异不显著（P〉0．05）。结论为在40日龄吉戎兔饲粮中添加80mg/kg蛋氨酸锌适宜。     

Evaluation of a serum-based PCR assay for the diagnosis of canine monocytic ehrlichiosis

The aim of this study was to estimate the relative diagnostic sensitivity and specificity of a polymerase chain reaction (PCR) assay in the serum of dogs with naturally occurring non-myelosuppressive canine monocytic ehrlichiosis (CME), and to investigate the association between PCR positivity and immunofluorescence antibody (IFA) titres for Ehrlichia canis. Serum samples obtained from 38 dogs with non-myelosuppressive CME and 12 healthy dogs were analyzed retrospectively. Each serum sample was analyzed in triplicate using an E. canis-specific nested PCR assay targeting a 389 bp sequence of the 16S rRNA gene. E. canis DNA was amplified in 24 of 38 (63.1%) affected dogs; all samples from healthy dogs were negative. A high level of agreement was found among the PCR replicates (P < 0.0001). Median IFA titre of the 24 PCR-positive dogs was significantly lower than that of the PCR-negative infected dogs (P = 0.0029), indicating that E. canis DNA may circulate prior to the development of a high antibody titre. Serum-based PCR analysis is suggested for the early diagnosis of CME when whole blood samples are not available.     

Kinetics of immune response to foot-and-mouth disease virus (type Asia 1) in experimental cattle

Humoral and mucosal (secretory antibody)immune response to FMDV type Asia 1 in cattle was analyzed after vaccination and infection using virus neutralizing test (VNT). Vaccination (1/16th the usual dose) failed to protect cattle from generalized clinical disease following experimental FMDV Asia 1 infection. Our results showed that infection induced higher and prolonged serum antibody titres indicating antigen mass is important for optimal immune response. Experimental FMDV infection induced significant secretory antibody (mucosal) response in cattle. Though, there was no difference in the serum antibody response between the cattle that developed generalized infection (unprotected) and those with only localized infection (protected), secretory antibody response differed, wherein the unprotected cattle had higher secretory response than protected cattle. Thus, FMDV Asia 1 infection stimulates a similar serum antibody response and a unique secretory antibody response among the infected cattle. An erratum to this article can be found at     

Effect of blood sample handling post-collection on Erysipelothrix rhusiopathiae antibody titres

A study was conducted to determine the effect of blood sample mishandling on the performance of an enzyme-linked immunosorbent assay for the detection of antibodies against Erysipelothrix rhusiopathiae. Eleven sample maltreatments (storage at −10 °C, storage at 4 °C, heat treatment of clotted blood, haemolysis, repetitive freeze–thaw cycling, and substitution of plasma in place of serum) were simulated in a laboratory environment and then run concurrently against a gold standard sample (storage at −80 °C).The mishandling treatment groups that simulated high levels of haemolysis had significantly lower optical density (OD) readings when compared to the gold standard. However, the magnitude of the effects was relatively small and only samples with OD values close to the cut-off changed state from positive to negative. Heat treatment had a minor, but non-significant, effect on OD values. Findings from this study suggested that immunoglobulin G antibody was stable in the face of most common sample mishandling events.