湖北省甘蓝产业发展现状分析

湖北省是蔬菜大省,甘蓝是湖北省蔬菜主产区的骨干品种。通过对湖北省甘蓝产业发展现状进行阐述,从3方面对湖北省甘蓝产业概况展开介绍:甘蓝在湖北省的地位、湖北省甘蓝区域化优势、品牌优势对湖北省甘蓝带动作用,并对湖北甘蓝品种茬口格局、甘蓝病虫害发生情况、甘蓝采后贮运销售情况进行介绍,列出了目前湖北省甘蓝产业发展存在的问题,并提出了对策及建议:着眼于全产业,提升产业竞争力;注重科技手段,提升育种能力;农机农艺结合,规模化高效种植;注重土壤管理,营造良好生长环境;加大科研力度,改善尾菜处理。     

甘蔗叶不同还田方式对土壤养分的影响

为减轻秸秆焚烧所带来的环境问题和充分利用甘蔗叶资源,在大田环境下动态监测甘蔗叶粉碎、焚烧和深埋3种还田方式对土壤养分的影响。结果表明:甘蔗叶粉碎还田和深埋还田有利于甘蔗叶中有机碳及氮、磷、钾养分的缓慢释放;甘蔗叶焚烧还田提高了土壤碱解氮含量5.6~20.9 mg/kg和速效磷含量0.8~4.0 mg/kg,促进土壤中氮磷向有效态转化,焚烧还田初期显著增加了土壤速效钾的含量12.3~18.3 mg/kg,但焚烧会损失部分甘蔗叶中碳氮元素,也造成环境污染。综合分析认为甘蔗叶粉碎还田是比较省工、方便的处理方式。     

miR-140-3p inhibits viability,invasion and migration of non-small-cell lung cancer A549 cells by targeting PD-L1

AIM: To explore the target relationship between microRNA-140-3p (miR-140-3p) and programmed cell death ligand 1 (PD-L1) and their effect on the viability, migration and invasion of non-small-cell lung cancer A549 cells.METHODS: RT-qPCR was used to detect the miR-140-3p expression in HLF-1, A549 and H1299 cells, and then the A549 cells with the most significant difference were selected as the subsequent research object. TargetScan software and dual-luciferase reporter assay were performed to predict and confirm the target relationship between miR-140-3p and PD-L1. RT-qPCR and Western blot were used to determine the effects of miR-140-3p mimic and inhibitor on PD-L1 expression level. MTT assay was used to detect the viability of A549 cells. Transwell assay was performed to detect the migration and invasion abilities of the A549 cells.RESULTS: miR-140-3p was significantly down-regulated in the A549 cells and H1299 cells (P<0.05). Transfection with miR-140-3p mimic decreased the expression of PD-L1 and inhibited the viability, migration and invasion of the A549 cells. Transfection with pcDNA3.0-PD-L1 reversed the inhibitory effect of miR-140-3p on the viability, migration and invasion of the A549 cells.CONCLUSION: miR-140-3p inhibits the viability, migration and invasion of A549 cells by targeting PD-L1.     

果蔬型甜玉米新品种京科甜191的选育

京科甜191是以超甜玉米自交系T68为母本,自交系T6302为父本,通过杂交选育而成的果蔬型甜玉米品种。果穗筒型,穗长19.0 cm,穗粗5.3 cm,穗行数16~18行,行粒数36~38粒,粒行整齐。籽粒亮黄色,外观漂亮。平均每667 m2鲜果穗产量900 kg左右,出籽率约67.4%。鲜籽粒含粗蛋白2.98%,粗脂肪1.37%,总糖6.7%,口感甜脆清爽,皮薄无渣。播种至采收平均85.4 d(天),适宜京津冀及类似生态区种植。     

2株毒死蜱降解菌的分离鉴定及其混合降解特性研究

从生产毒死蜱农药厂采集的活性污泥中分离筛选得到2株降解效率较高的毒死蜱降解菌,命名为D1、D3,根据表型特征、生理生化特性和16 S rRNA基因序列相似性分析,将其鉴定为苍白杆菌属(Ochrobactrum sp.)和副球菌属(Paracoccus sp.)细菌。2株菌以最佳配比(1∶1.25)混合施用时,与单菌相比,毒死蜱降解效率可提高12%~26%;以混合菌株为研究对象,发现其对毒死蜱最适降解温度为30℃,最适降解pH值为7.0,最适NaCl浓度为0.5%;混合菌株施入土壤后,可保持较高的定殖能力和降解效率。     

氮磷钾用量对小麦冠层不同层次光截获和干物质分配的影响

为明确不同氮、磷、钾用量对小麦冠层不同层次光截获和干物质分配的影响，以济麦22为供试材料，设置F0（不施肥）、F1（N 180 kg·hm^-2，P₂O₅ 75 kg·hm^-2，K₂O 60 kg·hm^-2）、F2（N 225 kg·hm^-2，P₂O₅ 120 kg·hm^-2，K₂O 105 kg·hm^-2）和F3（N 270 kg·hm^-2，P₂O₅ 165 kg·hm^-2，K₂O 105 kg·hm^-2）4个施肥量处理，比较分析开花后不同氮、磷、钾用量对小麦叶面积指数、冠层不同层次光截获特性和成熟期干物质分配的影响。结果表明，F1处理下叶面积指数显著高于F0处理，而与F2和F3处理间无显著差异；开花后15 d，F1处理下小麦冠层不同层次及总PAR截获率和截获量均显著高于F0处理，而与F2和F3处理间无显著差异。F1处理下成熟期干物质在小麦冠层不同层次营养器官中的分配量、籽粒中的分配量及总干物质积累量显著高于F0处理，而与F2和F3处理间无显著差异。成熟期干物质在小麦冠层不同层次营养器官和籽粒中的分配量以及总干物质积累量与冠层上层（顶部至株高2/3）、中层（株高2/3至株高1/3）和总PAR截获率均呈显著正相关。F1处理（N 180 kg·hm^-2，P₂O₅ 75 kg·hm^-2，K₂O 60 kg·hm^-2）为本试验条件下的最优处理。     

Growth performance of Black Bengal,Saanen, and their crossbred F1 as affected by sex,litter size,and season of kidding

The objective of the present study was to determine the factors that influenced growth performance of the goat kids of Black Bengal (BB), Saanen (SA), and their crossbred F1 (male Bengal × female Saanen [BBSA] and male Saanen × female Black Bengal [SABB]). Data for 674 kids were analyzed from 316 litters and 134 does. All kids were weekly measured on their characteristics (body weight, length, height at the withers, and chest girth) from birth to 11 weeks old. The kid’s breed and sex, litter size, and season of kidding influenced birth weight and other characteristics through the experiment. The SA and BBSA kids showed similar performance, which were higher than BB and SABB kids. Male kids had higher performance than female kids, and kids from a single litter showed the highest performance. Kids born during rainy season showed lower performance than those born in hot and cool seasons. In conclusion, the crossbred BBSA is superior to SABB or BB to raise in tropical climate Moreover, sex, litter size, and kidding season also affected growth performance during the preweaning period up to 11 weeks old.     

白屈菜CmFAD2基因的克隆及植物转化载体构建

通过对白屈菜低温应答过程的转录组分析发现膜脂不饱和化相关基因的表达在一定过程中发生变化,脂肪酸去饱和酶基因FAD2在随温度的变化趋势为正"V"型,且表达量变化显著。利用NCBI等在线软件对序列进行相关生物学信息分析,并对白屈菜FAD家族成员FAD2基因的完整开放阅读框(ORF)进行克隆,并命名为CmFAD2。选用克隆载体pMD-19-T,转化大肠杆菌DH5α,测序验证序列正确性及完整性。将目的基因与植物表达载体pRI-201-AN连接构建重组DNA pRI-201-AN-Cm FAD2,电击法转化农杆菌LBA4404,利用菌液PCR法验证成功。该基因可作为药用植物抗寒品种创制的候选基因。     

Notch1 promotes renal tubulointerstitial fibrosis in renal tissues of diabetic mice by inhibiting autophagy through Akt/mTOR signaling pathway

AIM: To observe the changes of Notch1 expression and autophagy in the renal tissues of diabetic mice, and to explore the regulatory effect of Notch1 on tubulointerstitial fibrosis by inhibiting autophagy in diabetic nephro-pathy. METHODS: The mice were randomly divided into normal control group (db/m mice) and diabetes group (db/db mice), with 8 rats in each group. After 12 weeks of feeding, the mice were sacrificed and the corresponding biochemical indexes were measured. The protein expression of Notch1 in the renal tubular epithelial cells was observed by immunohistochemical staining. The protein levels of Notch1, PTEN, p-Akt (Thr308), Akt, p-mTOR (Ser2448), mTOR, LC3, P62, collagen type Ⅰ (Col-Ⅰ) and collagen type Ⅲ (Col-Ⅲ) were determined by Western blot. RESULTS: Compared with the db/m mice, the blood glucose, glycosylated hemoglobin, serum creatinine, triglyceride and total cholesterol were increased in the db/db mice (P<0.01). Renal tubular epithelial cell vacuolar degeneration, renal tubular expansion and interstitial inflammatory cell infiltration in db/db mouse renal tissues with HE staining were observed. The images of Masson staining showed collagenous fiber-like substance deposition in the glomerular capillaries and renal interstitium, and disarrangement of tubular structure in the renal tissues of db/db mice. The protein expression levels of PTEN and LC3-Ⅱ were decreased (P<0.01 or P<0.05), while the protein levels of Notch1, P62, p-mTOR (Ser2448), p-Akt (Thr308), Col-I and Col-III were increased in the db/db mice as compared with the db/m mice (P<0.01). However, no significant change of total mTOR and Akt proteins between the 2 groups was found. CONCLUSION: Notch1 protein expression was increased, PTEN expression was significantly reduced, Akt/mTOR pathway was activated, autophagy was inhibited, and fibrosis was aggravated in the renal tissues of the diabetic mice.