芒果蒂腐病菌对芒果采后乙烯释放和ACC含量的影响

测定芒果蒂腐病菌(Botryodiplodia theobromae)对芒果果实乙烯代谢的影响,结果表明:B.theobromae在芒果果肉培养基上不产生乙烯,但是B.theobromae代谢活动能诱导芒果果肉中产生大量的1-氨基环丙烷-1-羧酸(ACC),导致芒果快速释放乙烯,随着发病程度增加和贮藏期延长,乙烯释放速率快速增加。高浓度乙烯使芒果果肉快速软化,在25℃条件下,果实在发病后3～5 d就完全腐烂。     

MrMYB1-MrbHLH1:一个有潜力的园艺植物转基因可视化报告基因

将杨梅果实中参与花色苷生物合成的转录因子编码基因MrMYB1和MrbHLH1同时搭载到双元表达载体的多克隆位点内,重组成双价表达载体,通过农杆菌介导的叶盘法将其分别转入烟草、番茄和森林草莓,成功转化MrMYB1-MrbHLH1的烟草、番茄和森林草莓不定芽因大量积累花色苷而呈红色,与野生型或假阳性不定芽相比,表型差异明显;转化35S::MrMYB1-MrbHLH1的烟草和番茄植株中可显著积累花色苷呈红色,且该性状可稳定遗传。MrMYB1-MrbHLH1在3种园艺植物具有调控花色苷积累而使阳性转化植物部分组织器官呈现红色的功能,是1个有潜力的遗传转化可视化报告基因。     

USP14 regulates H2O2 induced oxidative stress in H9c2 cells

AIM:To evaluate the effect of inhibiting ubiquitin-specific protease 14(USPl4) activity on oxidative stress induced by H₂O₂ of H9c2 cells.METHODS:The H9c2 cells were incubated with H₂O₂ at 25 μmol/L for 2 h to establish the oxidative stress injury model.The cells were divided into control group,H₂O₂ group,IU1 group (25 μmol/L or 50 μmol/L) and IU1+H₂O₂ group.The H9c2 cells activity was measured by MTS assay.The level of intracellular reactive oxygen species (ROS) and cell survival rate were analyzed by flow cytometry assay.The changes of the mitogen-activated protein kinase (MAPK) family related proteins were detected by Western blot.RESULTS:Compared with control group,the cell activity and the viability rate in H₂O₂ group were decreased (P<0.05),while the intracellular ROS,the protein levels of Bax/Bcl-2,P53,p-ERK1/2,p-JNK and p-P38 were increased (P<0.05).Compared with H₂O₂ group,the cell activity and the viability rate of the H9c2 cells in IU1+H₂O₂ group were increased (P<0.05),while the intracellular ROS,the protein levels of Bax/Bcl-2,P53,p-ERK1/2,p-JNK and p-P38 were decreased (P<0.05).CONCLUSION:Inhibition of USPl4 activity reduces the oxidative stress injury of the H9c2 cells.The mechanism may be related to inhibition of the MAPK signaling and down-regulation of apoptosis related proteins.     

荞麦剥壳效果在线检测装置的设计与试验

为提高荞麦剥壳设备的自动化程度,改善以往依靠手工筛分、计算的方法得到碎米率、整米率和未剥壳率,且不能实时检测等问题,设计了一种基于图像识别技术的荞麦剥壳效果在线检测装置并进行了测试试验。该装置主要由取样机构、匀样机构、图像识别系统和气流除尘光照箱等组成。试验结果表明:碎米率均值误差为1.0 8%、整米率均值误差为-4.4 9%、未剥壳率均值误差为3.4 3%,能够较为准确地反映出荞麦加工过程中的剥壳效果。     

Succession and phylogenetic profile of eukaryotic communities in rice straw incorporated into a rice field: Estimation by PCR-DGGE and sequence analyses

Abstract

Succession and the phylogenetic profile of eukaryotic communities associated with rice straw decomposition in a rice field were studied using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis followed by 18S rDNA sequencing. Nylon mesh bags containing leaf sheaths or blades were buried in the plow layer of a rice field under flooded conditions after transplanting (Experiment 1) and under drained conditions during the off-crop season (Experiment 2). In addition, rice straw samples in Experiment 2 were taken out before plowing in spring and re-placed in the rice field under flooded conditions at transplanting. Statistical analyses based on DGGE patterns showed that eukaryotic communities were divided into two groups, namely group A before the placement in soil, after the mid-season drainage in Experiment 1 and under the drained conditions in Experiment 2 and group B before the mid-season drainage in Experiment 1 and under the flooded conditions in Experiment 2. Based on the sequence analysis of DGGE bands, which characterized the eukaryotic communities, succession of the communities was revealed, that is, most of the bands in group A were closely related to fungi, whereas the bands in group B were closely related to protozoa. These results indicated that eukaryotic communities associated with rice straw decomposition in the rice field are mainly affected by soil conditions, such as oxic or reduced conditions, irrespective of rice straw parts (leaf sheaths and blades).     

小鼠感染肝片吸虫早期IL-4/IFN-γ及M2巨噬细胞标记分子的变化

为弄清肝片吸虫感染早期的主要细胞免疫类型及选择性激活巨噬细胞(M2或AAMΦ)标记分子的变化,本试验采用肝片吸虫囊蚴为感染源,经口分别感染雌性BALB/c野生型小鼠,运用特异性PCR鉴定成功感染小鼠后用间接ELISA对腹腔中IL-4和IFN-γ的水平进行测定,并对细胞因子IL-4、转录因子GATA3和M2的标记蛋白Relm a、Ym1分子的mRNA进行荧光定量PCR检测.结果显示,在感染后1,3,5,7周,从所获取肝脏组织中扩增的ITS2片段条带清晰,大小正确,测序后确定为肝片吸虫ITS2序列,表明肝片吸虫感染成功.对腹腔中IL-4和IFN-γ的水平测定表明感染组IL-4在感染后1,3,5周比对照组的显著增加(P=0.013,P＜0.01,P=0.02),但在第7周时无显著差异.IL-4的水平显著高于IFN-γ(P=0.011),而在第7周两者间无显著差异；对腹腔中IL-4、GATA3、Relm α和Ym1 mRNA水平的测定结果表明,感染后IL-4和GATA3 mRNA水平在第3周达到最高峰；于感染后1,3,5,7周,IL-4和GATA3 mRNA水平都分别显著高于对照组(IL4:P=0.01,P=0.012,P=0.023,P=0.014;GATA3:P=0.011,P＜0.01,P=0.032,P=0.014),但IL-4和GATA3间无显著差异；Relm α和Ym1mRNA水平都分别显著高于对照组(Relm α:P＜0.01,P＜0.01,P＜0.01,P=0.011;Ym1:P＜0.01,P＜0.01,P＜0.01,P=0.012),且二者间无显著差异,并随时间推移mRNA水平都呈下降趋势,于第7周达到最低水平.本研究成功利用肝片吸虫-小鼠模型研究蠕虫感染早期细胞免疫类型及M2标记分子的变化,发现在肝片吸虫感染小鼠早期主要引起Th2为主的细胞免疫.IL-4和M2巨噬细胞标记分子Relm α和Ym1在感染后1,3,5,7周都显著增加且具有相似的变化趋势,但nelmα和Ym1在第1周的高表达可能受诸多因素的影响还有待深入研究.     

人IL-1Ra基因的克隆及原核表达的研究

IL-1Ra是第一个被发现的天然存在的细胞因子拮抗剂,结合于IL-1受体后不引起IL-1效应。文章通过RT-PCR从人的胎盘组织中克隆了人IL-1Ra基因,使其在大肠杆菌DH5α中重组表达。结果表明,克隆到了460bp左右的目的基因,并在大肠杆菌中成功表达了18ku左右的目的蛋白,目的蛋白在大肠杆菌中表达量占总蛋白20%左右,为IL-1Ra的进一步研究及应用奠定了基础。     

早熟茄子新品种蓉杂茄1号的选育

蓉 杂茄１ 号是 用地方品 种单株系 选的优 良自交系８５０１ 作 母本，８６０３ 作 父本配制 的早熟茄 子 一 代 杂 种 ， 果 实 长 棒 形 、深 紫 色、光 泽 度 好。 单 果 质 量 约 ２６５ ｇ ， 平 均 产 量 ３ ５００ ｋｇ ·（６６７ ｍ ２） － １ ，较 抗病，适 合于长江 流域栽培 。     

Effect of transketolase-like protein 1 on proliferation of human nasopharyngeal carcinoma cells

AIM: To investigate the effect of transketolase-like protein 1 (TKTL1) on proliferation of human nasopharyngeal carcinoma cells in vitro. METHODS: The siRNA against TKTL1 mRNA was constructed and transfected into human nasopharyngeal carcinoma cells (CNE cell line). The activity of transketolase was detected before and after RNA interference.Real-time PCR was used to determine the mRNA expression of transketolase (TKT) gene family in the CNE cells.Flow cytometry and MTT test were used to detect the effect of anti-TKTL1 siRNA on cell proliferation and cell cycle in the CNE cells. RESULTS: The total transketolase activity was significantly decreased in the CNE cells transfected with siRNA TKTL1 construct compared with the cells transfected with control vector or untransfected CNE cells. No significant difference in the expression level of TKT and TKTL2 gene between the CNE cells transfected with siRNA TKTL1 construct and the cells transfected with control vector or untransfected CNE cells was observed (P>0.05). However, the expression level of TKTL1 gene was significantly downregulated in the CNE cells transfected with siRNA TKTL1 construct compared with the cells transfected with control vector.Cancer cells were arrested in G0/G1 phase, and cancer cell proliferation was significantly inhibited in the CNE cells transfected with siRNA TKTL1 construct. CONCLUSION: TKTL1 plays an important role in the total transketolase activity and cell proliferation of human nasopharyngeal carcinoma. TKTL1 may be considered as a potential target for novel anti-cancer therapy.