Epidemiology of cashew anthracnose (Colletotrichum gloeosporioides Penz.) in Mozambique

Anthracnose of cashew (Anacardium occidentale) was studies on various genotypes and locations in Mozambique. Colletotrichum gloeosporioides was identified as the anthracnose causal agent using polymerase chain reaction. The relationships between incidence and severity of anthracnose on cashew genotypes were statistically analyzed by regression. Anthracnose leaf incidence, which is practically easy to evaluate, was consistently associated with leaf severity, and their relationships can be estimated using the restricted exponential function across locations, crop seasons, genotype and fungicide trials. Pooled data enabled estimation of initial incidence of 1.43% with percentage variance accounting for 83.2 and standard error of 8.3. By computing incidence data into the summary equation, 24 changes of 0, 1, 5, 10 and 40%, resulted in changes of severity estimates of 0.01, 0.05, 0.10, 0.50 and 1.00%, respectively. The maximum disease incidence was estimated as 80% when the severity reached only 5%. Increase in severity was observed afterward, approached a maximum of 25% when leaf detachment is observed. The use of incidence data for epidemic comparisons, genotype and fungicide evaluation in cashew orchards is recommended. Anthracnose incidence on leaves however, could not predict incidence on nuts.     

太原经济技术开发区用地效益评价

通过对太原经济技术开发区的实际调查和对2002—2011年10 a间的相关统计及国家商务部历年发布的国家级经济技术开发区发展报告的信息进行分析,从开发区土地利用现状、年度建设用地供给、科工贸收入、工业总产值、GDP、税收收入、国内外资金利用等方面,综合评价了太原经济技术开发区的经济效益和用地效益。结果表明,该区独立运行10 a来成绩显著,整体效益逐步提高,在山西所有开发区中经济效益名列前茅,在全省经济技术开发区中每年的综合评价都名列第1;与国内中西部或中部的同类开发区相比,该区经济总量和单位面积产生的效益排名中后;与国内先进经济技术开发区相比,差距较大;该区内部分企业规模大、影响广泛,经济效益和用地效益均好,起到了带头和示范作用,但仍有部分企业规模小、投入水平低、建设周期长、产生效益低,影响了全区整体效益的发挥。建议开发区管委会引导入区企业加大投入,加快建设步伐,及早产生经济效益;尽快实施扩区计划,保证经济快速、健康发展;落实土地供给和退出政策,使有限的土地发挥更大的效益。     

甘肃迭部国有林区生态效益补偿机制的探讨

本文从白龙江主要流域的生态现状入手，着重介绍和分析了白龙江流域迭部国有林区生态效益补偿机制的现状和问题，提出了主要的解决办法。     

《毛泽东思想和中国特色社会主义理论体系概论》教学改革探讨

“概论”课理论性、现实性很强,在当前我国社会转型和改革攻坚深刻影响人们思想的形势下,如何讲好“概论”课对教师的教学理念、教学方法、技能和知识结构都提出了很高的要求.笔者在认真分析学生对思想政治理论课需求的基础上进行了一系列教学方法改革和实践：坚持理论联系实际,讲好讲活理论；采取互动教学方法,让学生更多的参与教学；灵活运用多媒体技术；通过实践教学促进课堂教学；举办多种专题讲座,拓展学生思维和视野.     

Evaluation the effect of subchronic feeding of transgenic cotton line (CKC1) on the faecal microbiota of albino rabbits

Recent studies have demonstrated a strong relationship between the intestinal microbiota and the host health. As such, consumers are increasingly becoming more concerned about the potential effect of certain foods/feeds, particularly of transgenic origin on the gut microbiota. Although the European Food Safety Authority has recommended in their guidelines, to study the effect of transgenic food/feed on host-microbiota, yet, few studies have focused on the evaluation of such effects mainly due to culturing difficulties. Therefore, this study was intended to evaluate the potential adverse effects of transgenic diet consumption on some specific gut microflora (Lactobacillus group, Bifidobacterium genus, Escherichia coli subgroup and Enterococcus genus) of rabbits. A total of forty-eight rabbits were randomly assigned into four groups and fed a diet containing a variable proportion of transgenic cottonseeds at 0, 20, 30 and 40% inclusion level, respectively. Changes in the specific or total faecal bacterial population were monitored at five different experimental stages (i.e. 0, 45, 90, 135 and 180 days) using both the traditional plate count method (TM) and quantitative real-time PCR (qPCR). No significant differences (p > .05) were observed concerning numbers of specific bacteria or total bacteria between the control and experimental groups, though qPCR showed numerically higher values in terms of 16S rRNA gene copies as compared to the values obtained from TM. However, such numerical differences were biologically insignificant (p > .05). Similarly, no significant variations were noticed in the calculated B/E (log₁₀ copies of Bifidobacterium per g faces/log10 copies of E. coli genome per g faeces) ratios in all the groups. All the ratios were in the range of 1.24 to 1.30 throughout the experiment, indicating a good balance of intestinal microflora and greater resistance to intestinal disorders. It is therefore concluded that feeding transgenic cottonseeds could not adversely affect the gut microflora of rabbits during a long-term study.     

葶苈子药材生态适宜性区划研究

对全国葶苈子药材的生态适宜性开展评价,为优质葶苈子药材的栽培与选育提供理论依据。通过网络资料查阅和实地采集,收集了葶苈子药材共839个样点信息,其中播娘蒿430个样点,独行菜409个样点,综合55个生态因子,应用GIS和Maxent模型分析葶苈子药材在全国的生态适宜性分布。建立的Maxent模型预测效果良好(训练集和验证集特征曲线下面积均大于0.9),影响全国葶苈子药材生态适宜性的主要环境因子是降雨、温度、海拔、酸碱度、植被类型、土壤亚类等;全国内中药葶苈子最适宜生境主要集中在河北、河南、山西、山东、陕西、宁夏、甘肃南部、四川西部等地。对葶苈子分布区进行了生长等级划分,为葶苈子药材资源的调查研究、人工种植、划分优质产地、资源调查提供理论依据。     

Molecular characterisation of Theileria equi and risk factors associated with the occurrence of theileriosis in horses of Punjab (Pakistan)

Theileria equi (T. equi) is an obligate intra- and extra-erythrocytic parasite that causes equine theileriosis (ET) in equids. Equine theileriosis is considered a notifiable disease of global significance, a major constraint to the international movement of horses, and endemic in many countries. This disease may be difficult to diagnose, as it can produce variable and nonspecific clinical signs. A cross-sectional study was designed for the molecular characterisation of T. equi and to investigate the associated risk factors of ET accompanied by its consequences on haematological and sero-biochemical parameters. A convenience sampling of 500 blood samples were collected from ET suspect horses from January to December 2017. PCR was performed on all blood samples targeting the 18S rRNA gene of T. equi followed by sequencing; 9% animals tested positive with confirmed sequences. The isolates of this study showed high homology with Cuban, Russian and Brazilian isolates of T. equi (accession numbers KY111762.2 , MG551915.1 and KY952237.1 , respectively). Based on multivariate analysis, the principal risk factors consisted of absence of dogs on the premises and presence of tick infestation. The haemato-biochemical parameters showed a decrease in granulocytes and erythrocytes, and an increase in lymphocytes, monocytes, mean corpuscular volume, mean corpuscular haemoglobin, mean platelet volume, glucose, phosphorus and aspartate aminotransferase in positive horses. This is the first study which identified ET in Punjab (Pakistan) using molecular techniques and risk factors together with the haemato-biochemical variations in horses.     

甘肃省天祝县草原补奖政策实施效果

草原作为重要的生态资产与生产资源,在我国生态文明建设和社会经济发展中具有重要的战略地位。党的十八大以来,我国将生态文明建设纳入“五位一体”总体布局之中,在草原牧区实施草原生态补奖政策,对草地生态保护、修复和助力牧区精准脱贫具有重要意义。本研究以甘肃省天祝藏族自治县为例,通过历年统计数据和查阅年鉴等方式获得相关资料,对两轮补奖政策实施效果进行梳理,从补奖政策资金、草地生产力及载畜量、生态效益、经济效益和社会效益等方面分析了政策实施前后变化情况。结果表明：1)两轮补奖政策实施后,天祝县草原生态环境逐步恢复,草地生产力达到2 162.28 kg·hm–2,相比补奖政策实施前提高了45.4%;2)畜牧业生产方式不断转变,理论载畜量增长了42.98万个羊单位,相比补奖政策实施前提高了54.3%;3)农牧民人均收入从补奖政策实施前的4 012元增加到8 265元。遵从“绿水青山就是金山银山”的生态发展理念,为进一步加强草地生态恢复,建议：1)在平衡禁牧下农户收入水平和生态补偿资金投入的基础上确定草原补奖补偿标准;2)加快培育现代农业产业,加大产业扶持力度,积极发展特色农牧业;3)建立健全放牧禁牧制度,科学制定草原合理利用计划。     

兽药中非法添加物检测及风险防范技术研究

本文总结了近年来兽药中非法添加的规律,分析成因;全面梳理现有检测标准,对非法添加物检测方法的检测对象和目标药物进行统计分析,研究检测方法发展及内在联系。在非法添加物检测方法提升和风险防范方面进行思考,提出筛查与确证方法并重、进一步扩大检测方法适用性、加强高效、高通量检测技术研究、拓展非法添加物检测品种范围;加强检查与宣传、疏堵结合,全面减少非法添加风险。