外源粪菌干预对受体猪肠道屏障功能的影响研究

为研究外源粪菌干预对受体猪肠道屏障功能的影响,试验选取初生重相近、胎次相同、同日出生的杜×长×大三元杂交哺乳仔猪12窝,随机分为2组,每组6窝(每窝10~12头)。试验组于1~10日龄隔天灌喂1 m L金华猪粪菌悬液进行外源粪菌干预;对照组灌喂等量无菌0.1 mol/L无菌磷酸盐缓冲液(PBS)。试验期间,仔猪自由哺乳采食和饮水,分别于12日龄和27日龄进行屠宰、采样。结果表明:外源粪菌干预可明显减少腹泻,提高受体猪的平均日增重和成活率(P0.05);外源粪菌干预能显著降低27日龄受体猪肠道隐窝深度(P0.05),显著提高肠道绒毛高度/隐窝深度(P0.05);外源粪菌干预能显著增加肠道杯状细胞数和12日龄肠道分泌型免疫球蛋白A阳性(SIgA~+)细胞数(P0.05),同时显著提高肠道黏蛋白Muc2、紧密连接蛋白ZO-1和Occludin表达水平(P0.05),而27日龄试验组的肠道SIgA~+细胞数与对照组无显著差异(P0.05)。综上可知,外源粪菌干预能通过增加肠道杯状细胞和SIgA~+细胞数、提高黏蛋白和紧密连接蛋白表达的方式调节受体猪肠道屏障功能,具有维持猪肠道功能稳态和促进肠道健康的作用。     

Effects of oral administration of different dosages of carvacrol essential oils on intestinal barrier function in broilers

Essential oils are widely used in the pharmaceutical, food and cosmetic industries, and many plant essential oils have shown that they have positive effects on broilers nutrition. This experiment was conducted to study the effects of orally administered different dosages of carvacrol essential oils on intestinal barrier function in broiler chickens. A total of eighty 28‐day‐old (1.28 ± 0.15 kg) ROSS 308 broilers were randomly allocated to four groups of 20 replicates each, with one chicken per replicate per cage, and all were fed with the same diet. Four experimental groups were orally administered 0, 200, 300 or 400 μl carvacrol essential oils at 18:00 hr every day during the 2‐week experimental period. As a result of which, the gene expression of the occludin, claudin‐1, claudin‐5, ZO‐1 and ZO‐2 in intestinal mucosa of small intestine (p < 0.05) and the goblet cell content in small intestine epithelium (p < 0.05) were significantly increased; test subjects with 300 or 400 μl carvacrol essential oils reduced the microbial counts of Salmonella spp. and Escherichia coli in the intestines (p < 0.05); Essential oils administration also significantly increased activity of the sucrase (p < 0.05) and lactase (p < 0.05) in intestinal mucosa. In conclusion, the carvacrol essential oils have positive effects on growth performance and intestinal barriers function of broilers; those effects may be related to the dosage, as administration of 300 or 400 μl was more effective than that of 200 μl.     

线性红利界下带干扰风险模型的破产概率

考虑到保险公司在实际经营中收益所具有的不确定性和分红策略，建立一类具有线性红利界和带随机扰动的双复合Poisson风险模型，利用鞅方法给出模型关于破产概率的一个定理及上界．     

基于PID神经网络的滑翔增程制导炮弹解耦控制系统设计

采用Wiener空间的两个算子以及相关的恒等式，提出了新的方法证明了关于高斯过程函数的中心极限定理，并给出了该中心极限定理的应用实例.     

房山区中南部沟域景观资源分析及规划探讨

本文从风景园林规划的基本目的入手,通过实践和调研房山区中南部沟域景观资源,分析地区内各景观体系类型,分类整合地区景观资源特征,挖掘房山中南部景观资源本身价值,并通过对房山区中南部地区进行上方花海沟域景观规划,系统研究房山区中南部沟域地区的景观建设发展方向,为深层综合开发、培育和保护当地景观资源提出改进措施,亦为房山区中南部的沟域经济的建设发展提供必要的理论依据。     

活化风蚀坑沙障固沙技术及植被恢复

风蚀坑活化形成的流动沙地直接危害草场,使土地逐渐丧失生产力。结合风蚀坑内风速变化特征,采取风蚀坑及边坡、风蚀坑积沙区沙障固沙技术,恢复风蚀坑植被。结果表明:风蚀坑及边坡设置黄柳沙障3 a,植被覆盖率达到27.0%,植物种为7种;PE纱网沙障植被覆盖率为31.0%,植物种为11种;PLA沙障植被覆盖率达到15.6%,植物种为4种;黄柳+PE复合沙障植被覆盖率达到75.3%,植物种为6种,而对照植被覆盖率为1.6%,植物种2种。在风蚀坑积沙区,设置沙障3 a后,PE纱网沙障植物种达到10种,黄柳+PE沙障为8种,PLA沙障为4种,黄柳沙障6种,而对照为1种;植被覆盖率分别达到50.3%、50.7%、22.7%、40.0%,而对照为4.0%。采取网格或带状PE沙障,带状黄柳+带状PE沙障对风蚀坑植被恢复具有良好效果。     

近30年华南中部可降水量的变化分析

本文利用NCEP/NCAR逐月可降水量的再分析资料,选取1983年1月-2012年12月共360个时次的数据,分析可降水量的变化趋势。得出结论：近30年内华南中部的可降水量总体上呈下降趋势；季节性变化明显,可降水量值呈夏高冬低趋势。     

内蒙古中南部先秦两汉时期人群龋病与生业模式初探

本文对内蒙古中南部先秦两汉时期11组古代人群的龋病状况做了统计与分析,在与我国北方地区16组古代人骨材料对比的基础上,进一步讨论了其生业模式与龋患的关系。龋病的罹患情况是反映人群摄入碳水化合物含量的有效指标,龋病与人类社会生产力发展以及农业出现相关,内蒙古中南部地区先秦两汉时期不同生业模式人群的碳水化合物食物摄取量的多寡决定了其龋齿发病率的高低,以农业经济为主的人群龋齿发病率最高,并随着时代发展而进一步增加;以畜牧或渔猎采集经济为生的古代人群龋病发病率较低;农牧兼营的混合经济人群龋齿发病率居中,并且因农业经济所占比重的不同,其变异范围较大。     

国家中心城市基础设施承载力评价及障碍因素诊断

[目的]定量分析国家中心城市2006—2017年基础设施承载力的具体情况,为促进国家中心城市建设良性健康发展提供科学依据。[方法]选取2006—2017年国家中心城市基础设施承载力面板数据,从压力和状态两个层面构建城市基础设施承载力评价指标体系。运用熵权TOPSIS方法和障碍度模型对2006—2017年国家中心城市基础设施承载力和障碍因素进行评价和诊断,并基于基础设施承载力评价结果,定量测度基础设施承载力状况并诊断其障碍因子。[结果] 2006—2010年国家中心城市的基础设施承载力水平总体上大幅度提升,2011—2017年基础设施承载力的增长速度有所减缓,北京、上海、成都、郑州、西安的基础设施承载力大幅度提高,广州市的基础设施承载力改善幅度最小;随着国家中心城市的人口持续增加,基础设施的承载压力也持续增加,通过障碍度模型分析发现制约国家中心城市基础设施承载力的主要因素为人均绿地面积、人均家庭年生活用水量和城市移动电话普及率。[结论]随着中国经济的发展,中心城市对人口的虹吸作用越加强烈,人均绿地面积、城市移动电话普及率以及人均家庭年生活用水量可能与城市的经济与人口规模产生脱钩现象,无法满足人们日常的基础设施需求。     

Whistle signal variations among three Indo-Pacific humpback dolphin populations in the South China Sea: a combined effect of the Qiongzhou Strait's geographical barrier function and local ambient noise?

Geographic variations in the dolphin whistles could be useful in assessing association and isolation among populations. Whistle of free-ranging Indo-Pacific humpback dolphins (Sousa chinensis) among the Pearl River Estuary (PRE), Leizhou Bei (LZB) and Sanniang Bay (SNB) populations were investigated. A total of 2850 whistles with legible fundamental contour were extracted and 15 acoustic parameters were measured. Contrary to SNB, PRE and LZB had the same relative proportion of tonal type compositions with flat and sine representing the most frequent types. The generalized linear model analysis showed significant acoustic difference among populations and tonal types. All frequency parameters in SNB were significantly higher than those in PRE and LZB, where no significant variation was observed in most of the parameters either at the population level or within each tonal type. Canonical discriminant functions analysis showed a smaller difference between PRE and LZB than between PRE and SNB and between LZB and SNB. Compared with previous recordings, recent recordings demonstrated a consistent pattern of becoming higher in whistle frequency parameters in both LZB and SNB populations, suggesting that noise pollution in LZB and SNB increasing with time according to the acoustic niche hypothesis. Dolphin whistle's geographic variations could be shaped by the combined function of the geographical barrier function of the Qiongzhou strait and local ambient noise. Considering the isolated condition and the relatively smaller population size of the humpback dolphin in the SNB, more effective and proactive conservation actions should be taken to prevent the extinction of small populations.