全文获取类型
收费全文 | 830篇 |
免费 | 92篇 |
国内免费 | 150篇 |
专业分类
林业 | 2篇 |
农学 | 317篇 |
基础科学 | 22篇 |
141篇 | |
综合类 | 271篇 |
农作物 | 237篇 |
畜牧兽医 | 11篇 |
园艺 | 4篇 |
植物保护 | 67篇 |
出版年
2024年 | 8篇 |
2023年 | 31篇 |
2022年 | 35篇 |
2021年 | 30篇 |
2020年 | 54篇 |
2019年 | 39篇 |
2018年 | 31篇 |
2017年 | 55篇 |
2016年 | 39篇 |
2015年 | 53篇 |
2014年 | 42篇 |
2013年 | 70篇 |
2012年 | 38篇 |
2011年 | 74篇 |
2010年 | 44篇 |
2009年 | 55篇 |
2008年 | 39篇 |
2007年 | 94篇 |
2006年 | 60篇 |
2005年 | 39篇 |
2004年 | 22篇 |
2003年 | 6篇 |
2002年 | 7篇 |
2001年 | 12篇 |
2000年 | 3篇 |
1999年 | 11篇 |
1998年 | 9篇 |
1997年 | 9篇 |
1996年 | 2篇 |
1995年 | 7篇 |
1993年 | 7篇 |
1992年 | 6篇 |
1991年 | 2篇 |
1990年 | 11篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有1072条查询结果,搜索用时 15 毫秒
1.
2.
Deanna L. Funnell Christopher B. Lawrence Jeffrey F. Pedersen Christopher L. Schardl 《Physiological and Molecular Plant Pathology》2004,65(6):285-296
Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic β-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to salicylic acid (SA), may be involved in regulation of PR-2d following induction of SAR with P. tabacina. This work provides evidence that regulation of PR-2 genes during P. tabacina-induced SAR may be similar to regulation of these genes during infection of N-gene tobacco by TMV or following exogenous application of SA, and provides further support for the role of SA in regulation of genes during P. tabacina-induced SAR. 相似文献
3.
小麦淀粉颗粒蛋-1(starch granule protein 1,即SGP-1)突变体是选育高直链淀粉含量、高抗性淀粉小麦品种的重要基础材料。SGP-1突变缺失,使胚乳中支链淀粉侧链的延伸受阻,从而使胚乳表观直链淀粉含量相对升高,所制食品的抗性淀粉含量也相应提高,具有重要的保健意义。本研究主要是利用SDS-PAGE方法,检测307份国内外农家种、育成种、高代品系及国内外黑小麦材料的SGP-1(SGP-A1、SGP-B1和SGP-D1)蛋白组成,进而发掘SGP-1突变体;并对突变体材料进行相关农艺性状和品质性状特点的分析,为育种利用及品质改良提供参考。结果共检测到4份SGP-1突变体,均为冬性材料,其中国外材料3份(CH62444、EAP74961和Amigo)、高代品系1份(05黑初8),分别为SGP-A1单缺体,SGPA1和-D1双缺体,SGP-A1和-B1双缺体,以及SGP-A1单缺体。在主要农艺性状方面,EAP74961、05黑初8和Amigo抽穗期与石4185(CK)相近,而CH62444则抽穗较晚,并且3份国外材料植株均较高。这4份突变体材料麸皮中的非水溶性戊聚糖含量相对于水溶性戊聚糖占绝对优势,而胚乳中非水溶性戊聚糖含量与水溶性戊聚糖含量则较为接近,但CH62444、Amigo和05黑初8麸皮中的总戊聚糖含量较高(分别为14.33%,15.20%和12.91%),而EAP74961面粉中的总戊聚糖含量(1.34%)较高。在淀粉组分含量方面,4份突变体材料差异不大。 相似文献
4.
利用5个锈病成株期抗性基因的KASP标记Sr2_ger9 3p、Lr34jagger、CSTM4_67G、Lr68-2、VPM_SNP和抗赤霉病基因Fhb1的KASP标记TaHRC-KASP,对云南省育成的42个小麦品种(系)进行检测,旨在筛选出含有目标基因的优异小麦种质,为云南省持久抗病小麦新品种(系)的选育提供材料。结果表明,4个材料含兼抗型成株抗锈病基因Lr34/Yr18/Sr57,频率为9.52%;6个品种(系)含兼抗型成株抗锈病基因Lr67/Yr46/Sr55,频率为14.29%;7个材料含抗慢叶锈病基因Lr68,频率为16.67%;含兼抗型成株抗锈病基因Sr2/Yr30和成株抗叶锈基因Lr37的材料各有1个,频率均为2.38%;未检测出含抗赤霉病基因Fhb1的品种(系)。云麦69、云麦75、云麦56、宜麦1号和宜麦3号等兼有2个成株期抗锈病基因,可作为今后云南持久抗锈病育种的抗源材料。 相似文献
5.
Xinying Zhang Yajing Wang Guirong Huang Fu Feng Xiaoying Liu Rui Guo Fengxue Gu Xin Hu Ziguang Yang Xiuli Zhong Xurong Mei 《Journal of Agronomy and Crop Science》2020,206(2):161-168
Great understanding of the genotypic difference in diurnal stomatal conductance (gs) pattern and the key determinants of the pattern is important for saving water by adopting cultivars appropriately. Fifteen wheat genotypes were studied under different soil conditions and various meteorological conditions with pot cultivation in rain shelter for two years. Genotype and air humidity were found to be key determinants of diurnal gs pattern. All genotypes under low relative humidity (LRH) and most genotypes under high relative humidity (HRH) displayed a gradual decline pattern from morning through the afternoon. Under moderate relative humidity (MRH), all genotypes present a single-peak curve pattern, but they differed in peak time, which may lead to unreliable gs comparison between genotypes and get ostensible contrasting materials. The stomatal conductance was significantly different among genotypes under LRH and the increased gs magnitude is also significantly different when it was compared between LRH and HRH. The present results provide new thinking for selecting and adopting appropriate cultivars with specific stomata traits for the area with various meteorological conditions. 相似文献
6.
Abir Dey Brahma Swaroop Dwivedi Ranjan Bhattacharyya Siba Prasad Datta Mahesh Chand Meena Raj K. Jat Raj Kumar Gupta Mangi Lal Jat Vinod Kumar Singh Debarup Das Ravi G. Singh 《Soil Use and Management》2020,36(3):429-438
Increasing soil carbon (C) in arable soils is an important strategy to achieve sustainable yields and mitigate climate change. We investigated changes in soil organic and inorganic carbon (SOC and SIC) under conservation agriculture (CA) in a calcareous soil of the eastern Indo-Gangetic Plains of India. The treatments were as follows: conventional-till rice and wheat (CT-CT), CT rice and zero-till wheat (CT-ZT), ZT direct seeded rice (DSR) and CT wheat (ZT-CT), ZTDSR and ZT wheat without crop residue retention (ZT-ZT), ZT-ZT with residue (ZT-ZT+R), and DSR and wheat both on permanent beds with residue (PB-PB+R). The ZT-ZT+R had the highest total SOC in both 0–15 and 15–30 cm soil layers (20% and 40% higher (p < .05) than CT-CT, respectively), whereas total SIC decreased by 11% and 15% in the respective layers under ZT-ZT+R compared with CT-CT. Non-labile SOC was the largest pool, followed by very labile, labile and less labile SOC. The benefits of ZT and residue retention were greatest for very labile SOC, which showed a significant (p < .05) increase (~50%) under ZT-ZT+R compared with CT-CT. The ZT-ZT+R sequestered ~2 Mg ha−1 total SOC in the 0–15 cm soil layer in 6 years, where CT registered significant losses. Thus, the adoption of CA should be recommended in calcareous soils, for C sequestration, and also as a reclamation technique. 相似文献
7.
化肥减施下有机替代对小麦产量和养分吸收利用的影响 总被引:7,自引:3,他引:4
8.
Unpredictable temperatures and rainfall associated with climate change are expected to affect wheat (Triticum aestivum L.) production in various countries. The development of climate-resilient spring wheat cultivars able to maintain grain yield and quality is essential to food security and economic returns. We tested 54 CIMMYT spring bread wheat genotypes, developed and/or released over a span of 50 years, in the field for two years under optimum sowing dates, as well as using two delayed sowing dates to expose crops to medium and severe heat-stress conditions. The grain yield and yield components were severely affected as the heat-stress increased. Two contrasting groups of 10 lines each were identified to determine the effect of heat-stress on bread-making quality. The first set included entries that produced high yields in optimal conditions and maintained higher yields under heat-stress (superior-yielding lines), while the second set included genotypes that did not perform well in the environment with high temperature (inferior-yielding lines). We identified genotypes exhibiting bread-making quality stability, as well as the quality traits that had higher correlation with the loaf volume in the environment without stress and under heat-stress. Of all the quality traits tested, dough extensibility (AlvL) and grain protein content had a significant influence in heat-stress adaptation. Most of the lines from the superior-yielding group were also able to maintain and even improve quality characteristics under heat-stress and therefore, could be used as parents in breeding to develop high-yielding and stable quality wheat varieties. 相似文献
9.
针对晋阳345和晋麦73两个定型品种存在抗寒性较差的缺点,对其幼胚愈伤组织进行了4种处理:1、愈伤组织在MD培养基上继代2~3次;2、愈伤组织在紫外灯下约10cm照射2h;3、愈伤组织在低温2℃和常温25℃下交替培养30d;4、愈伤组织在紫外灯下照射2h后,再在低温2℃和常温25℃下交替培养30d。结果表明,4个处理后代R2主要农艺性状的变异率差异显著,且紫外灯照射幼胚愈伤组织的变异率比低温2℃和常温25℃交替处理幼胚愈伤组织高,其后代各种农艺性状的变异范围较大,变异率较高。但后者有利于改良抗寒性较差的定型小麦品种。 相似文献
10.