云南小麦品种（系）锈病和赤霉病抗性功能基因的KASP标记检测

利用5个锈病成株期抗性基因的KASP标记Sr2_ger9 3p、Lr34jagger、CSTM4_67G、Lr68-2、VPM_SNP和抗赤霉病基因Fhb1的KASP标记TaHRC-KASP,对云南省育成的42个小麦品种（系）进行检测,旨在筛选出含有目标基因的优异小麦种质,为云南省持久抗病小麦新品种（系）的选育提供材料。结果表明,4个材料含兼抗型成株抗锈病基因Lr34/Yr18/Sr57,频率为9.52%;6个品种（系）含兼抗型成株抗锈病基因Lr67/Yr46/Sr55,频率为14.29%;7个材料含抗慢叶锈病基因Lr68,频率为16.67%;含兼抗型成株抗锈病基因Sr2/Yr30和成株抗叶锈基因Lr37的材料各有1个,频率均为2.38%;未检测出含抗赤霉病基因Fhb1的品种（系）。云麦69、云麦75、云麦56、宜麦1号和宜麦3号等兼有2个成株期抗锈病基因,可作为今后云南持久抗锈病育种的抗源材料。

Identification of Genes Associated with Rust Resistance and Fusarium Head Blight Resistance in Yunnan Wheat Cultivars(Lines) by KASP Assays

In order to provide resources for genetic improvement of wheat disease resistance in Yunnan Province by selecting valuable germplasm having disease-resistant genes using KASP assays Sr2_ger93p, Lr34jagger, CSTM4_67G, Lr68 2, VPM_SNP and TaHRC KASP which associated with 5 rust adult-plant resistance genes and Fusarium head blight resistance gene Fhb1, 42 Yunnan improved wheat cultivars (lines) were screened. The results showed that four cultivars carried pleiotropic rust resistance gene Lr34/Yr18/Sr57, with a frequency of 9.52%. Six cultivars (lines) carried pleiotropic rust resistance gene Lr67/Yr46/Sr55, with a frequency of 14.29%. Seven cultivars carried the slow-rusting gene Lr68, with a frequency of 16.67%. Olny one cultivars carried pleiotropic rust resistance gene Sr2/Yr30 or rust adult-plant resistance gene Lr37, with a frequency of 2.38%, respectively. Fusarium head blight resistance gene Fhb1 was not found in 42 tested wheat cultivars (lines). Yunmai 69, Yunmai 75, Yunmai 56, Yimai 1, and Yimai 3 carried two adult-plant resistance genes which could be used as resistant materials for genetic improvement of durable rust resistance in Yunnan Province.