ISSR Analysis of M_1 Generation of Gladiolus hybridus Hort Treated by EMS

Gladiolus hybridus Hort is one of famous cutting flowers,being famous of big size,bright color,various shapes and long bloom period.New species should be cultivated in order to meet consumers' needs.Mutagenic breeding is a shortcut to cultivate new species of flowers.In this study,corm bud of G.hybridus Hort was treated with different concentrations of EMS.Then M 1 generation was analyzed by ISSR.Results showed that EMS was a very effective mutagenic agent for the corm bud of G.hybridus Hort.With the increa...     

早熟苦瓜新品种春研2号的选育

春研2号苦瓜是以强雌系A94为母本、自交系B8为父本育成的早熟苦瓜一代杂种,表现早熟、丰产、连续结果能力强、抗逆性强、品质优等优良特性。果实青白色,棒形,单果约重400g,粒瘤,富有光泽,苦味适中,口感脆嫩,外观商品性及风味品质佳。可露地早熟栽培或春季保护地栽培,一般产量60000～90000kg/hm2,前期产量、总产量、品质性状优于蓝山苦瓜。     

秦岭林区雷击火的发生和预防

结合实践,分析了秦岭林区雷击火发生、蔓延的条件和趋势,提出了预防和扑救的技术与方法.     

植物耐水湿性研究综述

由于水岸造林植物经常遭水淹,成活率较低,因此其耐水湿性成为水岸景观防护林植物选择的难题,选择较耐水淹的植物是水岸造林的重点.本文对植物耐水湿性机理、耐水湿性评价指标和植物耐水湿的主要评价方法等方面的研究进展和发展趋势分别进行了阐述,并对今后的研究提出了一些看法.     

住房抵押贷款风险控制实证研究——基于中国银行某分行的数据分析

王先谦的《荀子集解》一书，是晚清诸子学的一部重要著述，是研究和整理《荀子》一书的集大成之作。王氏的学术成就主要体现在两个方面：一是他吸纳了清代学者中研究和整理杨倞注《荀子》一书最有代表性的成果，并对各家之说作了一个梳理和辨析；二是他个人对《荀子》以及杨注的校勘、注释、考辨等方面超出了前人的成就。     

肾型传染性支气管炎病毒M基因的遗传分析及其在杆状病毒表达系统中的表达

对肾型鸡传染性支气管炎病毒分离株Ck/Jiangsu/DS10/2008株(DS10株)膜蛋白(M)基因进行扩增、克隆和序列测定,并与GenBank中的其它25株参考株的M基因进行比对分析并构建进化树,研究其遗传进化关系。另将M基因克隆至杆状病毒表达系统pFastBac1载体并转染Sf9昆虫细胞进行表达,经间接免疫荧光鉴定,用琼扩试验验证表达产物的反应性,以研究M基因表达产物的生物学活性。测序结果表明DS10的M基因全长为678bp,编码225个氨基酸。遗传进化分析表明,DS10病毒与其它不同致病型的传染性支气管炎病毒共属同一大分支。间接免疫荧光检测表明,M蛋白在Sf9细胞中得到表达,琼扩试验验证表达产物具有良好的反应性,以上结果表明所表达的M蛋白具有良好的生物学活性。本研究表达的M蛋白可为其生物学活性研究及其诊断抗原、新型疫苗开发提供基础材料。     

两种中药方剂对奶牛感染性腹泻的治疗效果

为探讨两种中药方剂对奶牛感染性腹泻的治疗效果,选择河北省保定市某奶牛场人工感染轮状病毒(RV)和牛病毒性腹病毒(BVDV)的成年牛和犊牛为治疗对象。试验犊牛和成年牛均随机分为治疗组和对照组,各治疗组分别用两种中药方剂进行治疗,采用煎汤灌服,犊牛100g/次～150g/次,成年牛300g/次～500g/次,每天1次,连用3d～5d。对照组按牛场常规使用病毒唑及庆大霉素进行治疗。结果两种中药方剂对犊牛腹泻治愈率为70%～76%,有效率为90%～94%;成年病例治愈率为74%～80%,有效率为92%～96%。两种中药方剂对犊牛及成年牛感染性腹泻治疗效果明显优于临床上常用的抗病毒化药或抗生素(P<0.05)。     

脉冲放电杀菌技术及其应用研究进展

脉冲放电杀菌技术是一种利用电压瞬间发生变化而对细菌产生不可逆损失,进而导致死亡的一种非热杀菌技术。论文综述了脉冲电场的杀菌机理、特点和杀菌效果以及其在国内外应用的研究进展,提出脉冲放电杀菌技术有望取代传统的化学试剂灭活病原体方法,为动物传染病病原体的灭活和疫苗的制备带来革新。     

Single nucleotide polymorphisms of TLR4 locus in Chinese Cantonese population

AIM: Toll-like receptor 4 (TLR4) was an important pathogen recognition receptor in the innate immune system. The aim of this study was to investigate the distribution of TLR4 polymorphisms in the general population of China. METHODS: Peripheral blood samples were collected from 191 unrelated healthy Chinese Cantonese individuals. The functional regions of TLR4 locus, including promoter region and all three exons with their surrounding intronic regions were amplified using polymerase chain reaction. After purified, the amplified products were directly sequenced on both strands. RESULTS: A total of eight single nucleotides polymorphisms (SNPs) were detected, five of which were novel. The most common SNP were -1607 C/T with the minor allele frequency of 0.283. Two nonsynonymous substitutions Asp299Gly and Thr399Ile, which were common in Caucasus, were not detected in Cantonese. Neutrality test revealed that TLR4 in Chinese Cantonese was not significantly deviated from the neutral model. CONCLUSION: This is the new finding on the distribution of TLR4 SNPs in the general population of China. It provides several ethnic specific SNPs for further disease association studies of TLR4 polymorphisms in Chinese populations.