基于农业机械替代作用的农业劳动力需求预测模型

农业机械对农业劳动力具有替代作用,在不考虑其他因素对劳动力影响的前提下,我国农业劳动力占总人口的比重与农业机械拥有量呈反相的线性相关关系。检验结果表明,用最小二乘回归法建立的劳动力预测模型是可靠的。     

鼻内镜下脑脊液鼻漏的治疗

目的总结鼻内镜下脑脊液鼻漏的治疗经验,探讨鼻内镜下定位和修复的方法。方法回顾分析6例脑脊液鼻漏病人诊治过程,对脑脊液鼻漏的定位及修复方法进行讨论。结果6例病人,男、女各3例,年龄28~45岁。医源性2例,外伤性2例,自发性脑脊液鼻漏2例。于鼻内镜下寻找漏出部位,发现位于颅前窝3例,位于蝶窦3例。以明胶海绵蘸生物蛋白胶加鼻黏膜修复4例,生物蛋白胶加肌肉、阔筋膜加脂肪、阔筋膜各1例。83%(5/6)1次修复成功、1例2次修复成功。结论鼻内镜下脑脊液鼻漏定位和修复具有方法简捷、疗效确切,推荐作为脑脊液鼻漏治疗的首选方法。     

花生品种抗黄1号高产栽培技术

采用播期(A)、穴粒数(B)、穴距(C)、纯氮量(D)四因素三水平的正交试验设计,研究抗黄1号的高产配套栽培技术,试验结果表明,各因素的产量效应排序为C>A>B>D,穴距和播期是影响抗黄1号产量的关键因子,其次是穴粒数,影响最小的是纯氮量。最优处理组合为A3B3C1D1,同时,还就各因素对产量结构、植株性状、生育进程等生理特性的影响作了简要分析。     

Effect of NS-398 on invasion of colon cancer HT-29 cells in vitro and its regulation by CD44v6 and nm23-H1 genes

AIM: To study the anti-invasive effect of NS-398 on colon cancer cell line HT-29 in vitro an its regulation by CD44v6 and nm23-H1 genes. METHODS: Flow cytometry was used to detect the expression of COX-2 and CD44v6 in HT-29 cells. MTT was used for cell survival rate tests. The modified Boyden chamber model was used for quantitative invasion assay. RT-PCR was used to detect the expression of nm23-H1 mRNA. RESULTS: Flow cytometry analysis showed that COX-2 was positive in HT-29 cells. NS-398 had significant inhibitory effects on invasion of HT-29 cells, which had no relation with its cytotoxicity. NS-398 down-regulated the expression of CD44v6 and up-regulated the expression of nm23-H1 mRNA. CONCLUSION: NS-398 has an anti-invasive effect on HT-29 cells in vitro. Down-regulation of CD44v6 and up-regulation of nm23-H1 may be its underlying mechanisms.     

猪白细胞介素-6基因的非融合原核表达研究

用DNA重组技术，将已克隆的猪IL-6基因阅读框片段插入非融合原核表达质粒pBV220中的适当位置，获得重组质粒pBVpIL-6，转化大肠杆菌DH5α后，42℃诱导阳性表达菌。经SDS-PAGE检测发现目的蛋白获得了高效表达，表达量约占总蛋白的20％。表达的蛋白以包涵体形式存在，包涵体经过变性溶解和复性后用酶联免疫吸附试验（ELISA）做定性定量检测。ELISA结果证实了所表达的蛋白为猪IL-6，复性后具有一定活性．并测出了复性后活性蛋白的浓度。     

银杏叶类胡萝卜素的研究

银杏叶和提取黄酮(GBE)生产过程中的废弃物含有的类胡萝卜素主要为叶黄素、紫堇新质、隐黄质、新黄质和其他胡萝卜素,是天然的着色剂,如能加以利用,是银杏产业资源综合利用和循环经济发展的途径之一。     

谷氨酰胺对高温下黄羽肉鸡增重及血液生化指标的影响

选用42日龄健康“岭南黄”黄羽肉鸡60只,饲养于32±2℃的封闭舍内,随机分成4组,A组为对照组,饲喂基础日粮,B、C和D组为试验组,分别在基础日粮中添加0.2%、0.5%和0.8%的谷氨酰胺,试验期14 d。试验结果表明,高温下在日粮中添加谷氨酰胺能提高黄羽肉鸡的平均日增重,其中添加0.5%谷氨酰胺的效果显著(P<0.05),其显著提高49、56日龄的血清总蛋白含量(P<0.05),添加0.2%谷氨酰胺显著降低56日龄的血清尿素氮含量(P<0.05),添加谷氨酰胺对血清中的葡萄糖含量无影响(P>0.05)。说明谷氨酰胺有缓解肉鸡高温应激的作用。     

商洛茶业发展中存在的问题和对策

商洛已有300多年的种茶历史,已经发展到0.853万hm2,是我国最北部的茶区,具备生产优质名茶的区位优势;但由于缺乏统一规划、品种混杂,投资少、规模小、产量低、品牌少、技术落后,使得综合效益较低;因此商洛茶叶未来的生产中必须注意适地适树、统一规划;强化市场意识,扩大生产规模;调整茶业结构,实施品牌战略;加大投资力度,规范管理体制。把茶业作为解决区域“三农”问题的突破口,最终实现区域自然-经济-社会协调发展。     

一类p-Laplacian方程非平凡解的存在性

用山路引理得到了一类带有Dirichlet边值条件的p-Laplacian方程的非平凡解的存在性及多解性.     

Effects of Carbon Dioxide Insufflation Combined With Changes in Body Position on Blood Gas and Acid-Base Status in Anesthetized Llamas (Llama glama)

Objective— To study the combined effects of intra-abdominal CO₂ insufflation with changes in body position during laparoscopy in xylazine-ketamine-halothane anesthetized llamas. Study Design— Prospective, controlled study. Animals— Nine castrated, male llamas weighing 114 ± 23 kg, 3 to 13 years old. Methods— Three llamas (preliminary study [PS] group) were used to study the effect of right lateral, dorsal, and left lateral recumbency on gas exchange and acid-base status. The other six (experimental study [ES] group) were used to study the combined effects of changes in body position and CO₂ insufflation to an intraabdominal pressure of 10 to 12 mm Hg. Heart rate, respiratory rate, and indirect arterial blood pressures (systolic [SAP], mean [MAP], and diastolic [DAP]) were recorded every 5 minutes during anesthesia. Arterial blood gases (PaO₂ and PaCO₂) and acid-base status (pHa and HCO₃) were measured immediately after induction of anesthesia and before each change of position. Results— In the PS group, significant decreases in SAP, MAP and PaCO₂ and increases in PaO₂ and pHa were observed when the llamas were turned from right lateral to dorsal recumbency. Values for HCO_-3 were lower than the postinduction values, but they remained unaffected by the changes in position. In the ES group, values for MAP were significantly lower when the llamas were placed in dorsal and left lateral recumbency than those observed during right lateral recumbency. Arterial O₂ tension during right lateral recumbency was lower but returned to preinsufflation values when the llamas were placed in the dorsal position. All llamas recovered uneventfully within 30 minutes after termination of anesthesia. Conclusions— Insufflation of CO₂ and changing body position induce minor and transient changes in cardiovascular and respiratory function. Clinical Relevance— Laparoscopy with mild intra-abdominal CO₂ insufflation (10 to 12 mm Hg) can be used safely in spontaneously breathing llamas anesthetized with xylazine, ketamine, and halothane.