小麦新品种豫农416优良特性及应用前景分析

豫农416是集高产、优质、适用性广于一体的小麦新品种,2009年通过河南省农作物品种审定委员会审定,审定编号:豫审麦2009001.为合理利用小麦新品种豫农416提供理论依据,利用2007—2009年河南省小麦良种区域试验和生产试验资料对豫农416的主要特性进行分析.结果表明:豫农416具有丰产性突出、稳产性好和适应性广等特点.2年区试平均产量比对照品种增产4.58%,生产试验比对照周麦18增产7.4%;变异系数均值8.71%(低于对照品种);适应度均值为87.5%(高于其他继试品种);主要品质达强筋优质小麦标准.该品种适合河南及周边省份相近生态区高中肥田种植,推广应用前景广阔.     

高校科技创新影响力研究——以河南工业大学为例

高校科技创新其特色和优势成果是提升高校影响力的"源动力";成果推广应用是提高高校影响力的"生产力";积极参与有利于提高高校社会影响力;科技创新平台有利于加强高校科技竞争力;抓住省局共建历史机遇,推动高校科技发展。     

新城疫病毒HN蛋白单克隆抗体的制备

为制备新城疫病毒HN蛋白的单克隆抗体,本研究采用新城疫病毒CK/AH/100/2010作为免疫原,免疫6~8周龄BALB/c小鼠,3次免疫后取脾细胞与SP2/0融合,用血凝抑制(HI)方法检测筛选,具有HI活性的单抗有6株:PX1-PX6。6株单抗与AIV、EDS等其它具有血凝活性的病毒的交叉血凝抑制试验结果表明,6株单抗具有良好的特异性。间接免疫荧光试验结果表明,PX1、PX3可与新城疫病毒CK/GD/263/2010 HN抗原发生特异性反应,而其它单抗不反应。     

新城疫病毒F_(48)E_9株重组NP蛋白单克隆抗体制备及鉴定

制备新城疫病毒(NDV)NP蛋白单抗,以期为NDV抗原表位研究及检测方法建立方面奠定基础。利用NDV F48E9株pET32a-NP重组蛋白免疫BALB/c小鼠,采用杂交瘤细胞技术,间接ELISA筛选,获得了2株稳定分泌抗NP重组蛋白单克隆抗体的杂交瘤细胞株,分别命名为1G3、4B12。经间接ELISA测定,腹水抗体效价分别为1 2.56×105、1 5.12×105。亚类鉴定结果表明这两株单抗均为IgG1。Western blot分析结果显示,1G3、4B12均能特异性识别重组NP蛋白。与NDV感染细胞经间接免疫荧光试验检测均呈黄绿色荧光。经相加ELISA测定表明两株单抗识别的抗原表位不同。     

湖南省油菜施肥状况调查

采用农户抽样调查的方法,分析研究了湖南省油菜施肥状况及存在的问题.结果表明,油菜种植中有机肥的施用比例为87.7％,施用最普遍的是人粪尿和猪粪尿.化肥品种中,尿素和复合肥的施用比例分别为53.5％和51.8％,而单质磷、钾肥及硼肥的施用比例较低.全省油菜氮、磷、钾肥平均总投入量分别为132 kg/hm2、47 kg/h...     

三结合育种方法在小麦优异种质创育中的应用

为创建新型的小麦基因体系,并为性状改良和新品种选育提供丰富的种质资源,利用远缘杂交、多倍体育种和电离辐射三结合的育种方法,进行了37 a的小麦超亲遗传及新种质创育工作。结果表明,三结合育种后代品系的染色体数量变异和结构变异幅度大,趋向双亲性状类型的分离较少而超亲遗传突出,后代种质分离持续时间长,不少具有较好的综合性状,籽粒产量和品质较常规育种后代显著提高。研究证明,三结合育种方法既克服了单纯的远缘杂交、多倍体育种或辐射育种方法的缺点,又综合了它们的优点,为丰富小麦种质资源和提高小麦育种效率开辟了一条新途径。     

Temporal dynamics of light and nitrogen vertical distributions in canopies of sunflower, kenaf and cynara

To enhance eco-physiological and modelling studies, we quantified vertical distributions of light and nitrogen in canopies of three Mediterranean bio-energy crops: sunflower (Helianthus annuus), kenaf (Hibiscus cannabinus) and cynara (Cynara cardunculus). Field crops were grown with and without water stress in 2008 and 2009. Canopy vertical distributions of leaf area index (LAI), photosynthetically active radiation (PAR), specific leaf area (SLA), nitrogen concentration (N_conc) and specific leaf nitrogen (SLN) were assessed over time for each crop × year × water input combination. Light and nitrogen distributions were quantified by the Beer's law (exponential model) and extinction coefficients for light (K_L) and nitrogen (K_N) were calculated. Within a year, K_L did not change significantly over the studied period in all irrigated crops, but differences in K_L were significant between years (sunflower: 0.74 vs. 0.89; kenaf: 0.62 vs. 0.71; cynara: 0.77). K_L estimates were always lower (−48 to −65%) in water-stressed sunflower and kenaf crops because of the reduction in leaf angle. These results should be taken into account, when simulating water-limited biomass production. Vertical SLN distributions were found in canopies when LAI was >1.5 (40 from 51 cases). These distributions were significantly correlated with the cumulative LAI from the top (r² = 0.75-0.81; P < 0.05), providing parameters to upscale photosynthesis from leaf to canopy levels. Vertical SLN distributions followed species-specific patterns over the crop cycle and varied less compared to PAR distributions between years. Lastly, we observed strong associations between SLN and PAR distributions in irrigated sunflower and kenaf canopies (r² > 0.66; P < 0.001). However, observed SLN distributions were less steep than the distributions that would maximize canopy photosynthesis.     

Effects of dietary copper sources on growth,tissue copper accumulation and physiological responses of Japanese sea bass (Lateolabrax japonicus) (Cuvier, 1828) fed semipurified or practical diets

An 8‐week trial was conducted to compare the efficacy of a chelated copper (Cu) with the inorganic Cu (CuSO₄), in the diet of Japanese sea bass (Lateolabrax japonicus). Six diets were prepared based on two basal diets (semi‐purified or practical) at two Cu inclusion levels (0 and 4 mg kg^?1 from or chelated‐Cu), accordingly named as S0, SI4, SM4, P0, PI4 and PM4 respectively. Each diet was fed to quadruplicate groups of Japanese sea bass (initial weight: 65.0 ± 0.03 g) during the trial. Fish fed practical diets had higher feed intake, specific growth rate, protein efficiency rate (PER), liver Cu accumulation, liver Na⁺K⁺‐ATPase and plasma alkaline phosphatase, but lower hepatosomatic index than the groups fed semi‐purified diets (P < 0.05). Higher PER was exhibited by fish fed chelated‐Cu diets than that of fish fed CuSO₄ diets (P < 0.05). Although liver Cu accumulation was significantly higher in fish fed practical diets,, liver Cu content increased significantly with increasing dietary copper levels only in fish fed semipurified diets. Chelated‐Cu has higher efficiency than CuSO₄ when fed in practical diets. Poor growth performance of fish fed semipurified diets might induce underestimating the nutrient requirement of Japanese sea bass.     

Operational Analysis of Open Untreated Sewage Source Heat Pump System

The effective sewage intake technology is prerequisite for the untreated sewage source heat pump system to run steadily. In terms of the insufficiency of current sewage intake technologies, an open sewage intake integration technology is proposed. The mathematical model and the distinguish basis of critical state are established for the sewage intake characteristic by defining some important operating parameters, such as the blocking coefficient, the contamination density and the section coefficient. Furthermore, the change rule of each critical operation parameter is observed along with the various initial parameters. The result can provide the theoretical basis to design the open intake integration technology.     

外施硅肥与灌溉方式对草地早熟禾白粉病病情的影响

为探索硅肥与灌溉方式对草地早熟禾(Poa pratensis)白粉病的防治效果,试验通过室内盆栽研究了外施硅肥与根灌对草地早熟禾白粉病病情的影响。结果表明,根灌对草地早熟禾白粉病病害的抵制作用不明显并具分异性;对于患病较重的禾草,根灌会使病情加重。外施SiO2溶液可以显著减轻草地早熟禾白粉病的病情指数和病害程度(P＜0.05)。在试验设定的硅肥梯度(0、31、92、275 mg/m2)内,随着施硅量的增加,病情的减轻愈显著。