| 新城疫病毒HN蛋白单克隆抗体的制备 |
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| 引用本文： | 于杨,崔佳莹,丁晴微,崔鹏飞,李印,邓国华,任涛,陈化兰.新城疫病毒HN蛋白单克隆抗体的制备[J].中国畜牧兽医,2011,38(9):94-97. |
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| 作者姓名： | 于杨 崔佳莹 丁晴微 崔鹏飞 李印 邓国华 任涛 陈化兰 |
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| 作者单位： | 1. 华南农业大学兽医学院,广东广州 510642;2. 中国农业科学院哈尔滨兽医研究所,农业部动物流感重点开放实验室/兽医生物技术国家重点实验室,黑龙江哈尔滨 150001 |
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| 基金项目： | 国家自然科学基金(31072319) |
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| 摘 要： | 为制备新城疫病毒HN蛋白的单克隆抗体,本研究采用新城疫病毒CK/AH/100/2010作为免疫原,免疫6~8周龄BALB/c小鼠,3次免疫后取脾细胞与SP2/0融合,用血凝抑制(HI)方法检测筛选,具有HI活性的单抗有6株:PX1-PX6。6株单抗与AIV、EDS等其它具有血凝活性的病毒的交叉血凝抑制试验结果表明,6株单抗具有良好的特异性。间接免疫荧光试验结果表明,PX1、PX3可与新城疫病毒CK/GD/263/2010 HN抗原发生特异性反应,而其它单抗不反应。
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| 关 键 词： | 新城疫病毒 单克隆抗体 HN基因 HI |
| 收稿时间： | 2011-01-17 |
Preparation Monoclonal Antibodies of HN Protein Using Newcastle Disease Virus |
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| YU Yang,CUI Jia-ying,DING Qing-wei,CUI Peng-fei,LI Yin,DENG Guo-hua,REN Tao,CHEN Hua-lan.Preparation Monoclonal Antibodies of HN Protein Using Newcastle Disease Virus[J].China Animal Husbandry & Veterinary Medicine,2011,38(9):94-97. |
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| Authors: | YU Yang CUI Jia-ying DING Qing-wei CUI Peng-fei LI Yin DENG Guo-hua REN Tao CHEN Hua-lan |
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| Institution: | 1. South China Agricultural University, Guangzhou 510642, China;2. Animal Influenza Laboratory of the Ministry of Agriculture/State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China |
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| Abstract: | In order to develop monoclonal antibodies of HN protein of Newcastle disease virus,BALB/c mice were immunized with CK/AH/100/2010 virus,and spleens were used for fusion with SP2/0.Six McAb cell strains of PX1 to PX6 were determined by hemagglutinin inhibition(HI).The results of HI cross-reaction with AIV,EDS and other HA virus suggested that the six McAbs had excellent specificity with NDV.The McAbs PX1,PX3 were shown to react with the HN antigen of NDV CK/GD/263/2010 in indirect-immunofluorescence assay. |
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| Keywords: | Newcastle disease virus monoclonal antibody HN gene HI |
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