Expression of tissue factor,tissue factor pathway inhibitor in the atherosclerotic plaque on femoral artery of New Zealand rabbits

AIM: To observe the regulatory changes of tissue factor (TF),tissue factor pathway inhibitor-1 (TFPI-1) and tissue factor pathway inhibitor-2 (TFPI-2) associated with the formation of atherosclerotic plaque on femoral artery of New Zealand rabbits.METHODS: New Zealand rabbits were fed with high cholesterol diet and received femoral artery balloon injury to establish moderated atherosclerotic plaque animal model.These rabbits were classified into 8 weeks group,10 weeks group and 12 weeks group.The expressions of factor TF,TFPI-1 and TFPI-2 in femoral artery were detected by immunohistochemical methods at 8th,10th and 12th week.The positive areas of the slice were analyzed by semi-quantification system.Fluorescence quantification PCR (FQ-PCR) was used to detect the copies of mRNA of TF,TFPI-1 and TFPI-2 in each group.RESULTS: The expressions of TF,TFPI-1 and TFPI-2 determined by immunohistochemical methods were increased from 8th,10th to 12th week,respectively.In each group,the quantity of mRNA of TF and TFPI-1 at the 8th,10th and 12th week was soared,respectively.In each group,the quantity of mRNA of TFPI-2 did not change in early phase,but decreased at 12th week.CONCLUSION: In the process of the atherosclerotic plaque formation in rabbit femoral artery model,the expressions of TFPI-1 and TFPI-2 proteins are increased.However,the expression of TPFI-1 or TFPI-2 could not stave off the development of plaque formation.     

畜禽DNA指纹的研究与应用

综述了ＤＮＡ指纹技术的原理，方法，以及在个体（品种）鉴别，亲缘关系分析，群体遗传变异分析，连锁分析等方面的研究进展。     

在物理教学中构建学生"主动参与"的策略

教学过程应该是促进学生"自我发展"的变化过程.教学过程只有通过学习者本身的积极参与、内化、吸收才能实现.因此学生能否主动地参与成为教学成败的关键.     

新疆野苹果群体遗传结构和遗传多样性的SRAP分析

 采用SRAP标记，对中国新疆野苹果4个种下居群的群体遗传结构和遗传多样性进行了研究。结果表明：10对SRAP引物总共扩增了209条带，其中206条是多态性带（98.56%）。巩留群体、新源群体、霍城群体和裕民群体分别扩增了180、169、178和165条多态性带，巩留群体的随机交配杂合度（hs = 0.3037 ± 0.0058）最高，其次为霍城群体。UPGMA聚类分析和群体间遗传分化系数显示，巩留群体和新源群体之间，以及霍城群体和裕民群体之间，遗传关系最近，群体间遗传变异最低。新疆野苹果群体内遗传变异高于群体间，占总变异的87.9%，主坐标轴分析显示4个群体是相对独立，其中巩留群体和新源群体，霍城群体和裕民群体之间，有较高的基因交流。所有参数分析表明，巩留群体遗传多样性最丰富，故在制定新疆野苹果原地和异地种质保护计划时应优先考虑巩留群体。     

高频饮用牛奶和葡萄糖对雌性大鼠生殖机能的影响

选用22d断奶SD大鼠高频饮用牛奶或葡萄糖40d,分别记录各组动物的阴道开张时间和阴道黏液、每周日增重、卵巢及子宫所占体重百分比、血糖水平,收集卵巢进行组织学处理以观察卵泡发育情况,探讨高频饮用牛奶和葡萄糖对雌性大鼠初情期的影响.结果显示,奶组和糖组大鼠较对照组阴道开张时间来得早,预示初情期可能提前,而且,奶组和糖组大鼠的卵巢占体重百分比较对照组高(P＜0.05).牛奶组血糖水平比对照组高,但糖组与对照组血糖水平差异不显著.通过对卵巢进行石蜡切片、HE染色及卵泡计数,结果显示:奶组的闭锁卵泡、每张切片卵泡总平均数都比对照组多(P＜0.05),而葡萄糖组的黄体数比对照组少(P＜0.05).试验表明高频饮用牛奶和葡萄糖可对性成熟前雌性大鼠的生殖机能产生一定影响.     

刺葡萄愈伤组织的诱导和分化培养

以刺葡萄叶片为材料，在1／2MS培养基上附加不同种类及浓度配比的植物激素，研究叶片愈伤组织的诱导及分化培养。实验结果表明：叶片愈伤组织的诱导较容易，最佳诱导培养基配方为以ZT1．0mg／L＋2，4-D2．0mg／L，诱导率可达100％，光培养条件下刺葡萄叶片愈伤组织的诱导率比暗培养的诱导率高。继代培养愈伤组织能诱导出根，但诱导出芽不易，刺葡萄叶片再生植株较难。增殖培养ZT以1．0mg／L＋NAA0．01mg／L为佳，1周增殖率可达1．292％。     

Locomotion Score and Its Impacts on Production Performance and Blood Routine Indexes in Chinese Holstein

To investigate the influencing factors of locomotion score (LS) and its impacts on production performance and blood routine index in Holstein,body condition score (BCS),LS and DHI (dairy herd improvement) of 1 486 lactating cows from two dairy farms,and the blood routine indexes of 101 lactating cows from one farm in Beijing were measured.The fixed model was used to analyze the impacts of herd,parity and lactation stage on LS,and the impacts of LS and BCS on daily milk yield,somatic cell score,milk composition and blood routine indexes in dairy cows.The results showed that both parity and lactation stage had significant impacts on LS.The LS increased with the increase of parit,the LS increased by 0.71 from first lactation to fifth and above lactation.The LS of cow in lactation stage Ⅲ(100-199 d) and Ⅵ (after 350 d) were significantly higher than cow in stage Ⅱ (45-99 d)(P<0.05),and both were higher than 2.The LS had no significant impacts on daily milk yield,milk composition and somatic cell score (P>0.05).However,the milk yield of the cow with LS≥4 was the lowest,and a decrease of 2.29 kg was found compared with the cow with LS=1.Among the various blood routine indexes,LS had significant impacts on red blood cell count,hemoglobin content,mean corpuscular hemoglobin concentration and lymphocyte count (P<0.05),and had no significant impacts on white blood cell count (P>0.05).With the increase of LS,the red blood cell count,hemoglobin content,mean corpuscular hemoglobin concentration and lymphocyte count of cows decreased with different extents.There were some problems on the body immunity and hematopoiesis of cows with poor LS.In dairy farm,regular evaluation of LS could be used to find the management problems of feet and legs,which had a great significance to reduce the loss of production performance and early culling caused by hoof disease.     

口服硫酸安普霉素在猪体内的药代动力学和生物利用度研究

对 5头健康猪口服和静脉注射国产硫酸安普霉素 ,研究其在猪体内的药代动力学和生物利用度。用微生物法测定血清药物浓度 ,结果平均回收率为 99.0 3%,血清最低检测浓度为 0 .0 5 μg/ ml,日内日间变异系数为 2 .2 %～ 5 .0 %,且血清浓度在0 .0 5～ 3μg/ m l范围呈良好线性关系 (r=0 .996 5 )。以 2 0 mg/ kg口服和以 2 0 mg/ kg静脉注射硫酸安普霉素后 ,经 Mcpkp药代动力学计算机程序处理 ,体内药物运转分别符合开放型一室和二室模型 ,生物半衰期 t1 / 2 分别为 (7.36± 1 .5 2 ) h和 (3.1 7± 0 .75 )h;CLB分别为 4 .82 L / kg· h和 0 .1 6 L / kg· h;AUC值分别为 4 .1 4和 1 30 .6 2。口服 :Cmax为 (0 .2 4± 0 .0 3)μg/ ml;Tp为 (5 .1 2±0 .6 1 ) h;T1 / 2 K为 (7.36± 1 .5 2 ) ;生物利用度 (AUCp.0 / AUCi.v)为 (3.1 92 8± 0 .70 4 4 ) %。上述药代动力学数据为动物临床用药提供有价值的理论依据     

建构观下教师角色的定位

在建构主义理论的指导下,教师必须改变机械的和静态的数学教学观,向动态的、易谬(经验主义和似经验主义)的数学观念转化。要重新认识教师在教学过程中的主导作用,教师不再是简单的知识传授者,而应是数学建构活动的激励者、组织者、参与者和导向者。