土壤参数监测系统的设计与实现

针对目前我国土壤参数监测存在的问题,设计了一套土壤参数综合监测系统.通过应用数据采集卡与PLC串行通信相结合的方式实现了数据的检测以及自动存取,在数据处理时采用Delphi6.0本身功能及桌面地理信息系统软件Arcview实现了统计图生成、报表打印、分布图生成等功能.     

强化食品中活性钙提取条件的商榷

研究应用ＥＤＴＡ法测定强化食品中活性钙提取条件的选择问题，提出用酸液提取活性钙的方法。     

猪圆环病毒2型感染细胞凋亡研究进展

细胞凋亡是多种因素调节的细胞主动死亡过程,细胞凋亡的两条主要通路分别是死亡受体介导的凋亡途径和线粒体凋亡途径,但它们共同的凋亡效应物为胱冬酶;理化因素如放射线、紫外线和高温等及生物学因素如基因表达产物、酶和细胞因子等都影响细胞的凋亡。肿瘤坏死因子和胱冬酶在猪圆环病毒2型感染细胞凋亡中起着非常重要的作用;p53诱导环-H2J、un氨基端激酶,NF-κB等均参与细胞凋亡。生产中,可采用猪圆环病毒2型疫苗免疫,干扰RNA的使用和减少应激等措施降低PCV-2感染,抑制细胞凋亡。     

北京市密云地区春季芦笋十四点负泥虫发生规律与温度关系研究

十四点负泥虫是北京市密云地区芦笋生产过程中主要害虫之一,也是影响全县芦笋生产、影响农民增收致富的主要制约因素。春季是十四点负泥虫发生危害关键期,如果防治时间不当,防治不及时,将会给整个芦笋生产造成重大损失,为此开展了十四点负泥虫在密云县春季出土危害与温度关系调查,明确了当土壤深5cm、06:00的温度6.0℃、下午的温度20.0℃时,十四点负泥虫开始大量出土活动;4月底-5月初芦笋出笋期,10:30-16:30为十四点负泥虫出土活动高峰期,此阶段开展防治效果最为理想。     

动态矩阵控制在电阻炉温度控制系统中的应用

通过对电阻炉动态对象特性的研究以及预测控制方法的改进，比较了动态矩阵控制和PID控制在电阻炉温度控制系统中的应用效果．仿真结果表明：DMC不但克服了建模的不精确性和参数的时变性，而且根据给定的期望曲线，实现了比PID控制更令人满意的性能指标．     

白鲜碱体外抗白色念珠菌活性研究

白色念珠菌等真菌是能够引起人类及动物患病的重要致病菌。本实验测定了白鲜碱对临床22株临床分离氟康唑耐药白色念珠菌的最小抑菌浓度（MIC），采用棋盘式微量稀释实验测定了两药联合应药的分级抑制浓度指数（ractional inhibitory concentration index，FICI）值。结果表明，白鲜碱有较好的抗白色念珠菌活性且与氟康唑有协同作用。     

K均值和分层聚类法在大米产地鉴别中的应用

大米称誉为"五谷之首",是我国的主要粮食作物。大米产地鉴别方法的研究对于保护广大消费者和生产厂家的利益、推动我国大米出口业的发展等都具有重要意义。为此,选取了19个响水大米样品和10个非响水大米样品,使用等离子体原子发射光谱的方法测定了样品中P,B,Zn,Fe,Cu,Mn,Na,K,Mg和Ca等10种微量元素的含量,并利用样品中的微量元素含量数据,使用K-Means聚类法和Hierarchical聚类法对大米样品进行了聚类分析。结果表明,K-Means和Hierarchical聚类法对于样品有聚类趋势。研究结果能够为大米生产厂商与质检部门鉴别大米产地提供参考和借鉴。     

太空春石斛组培快繁黄苗现象成因初探

以太空春石斛丛生芽为试验材料,初步研究了基本培养基、激素和附加物质香蕉对黄苗现象的影响。试验结果表明:基本培养基以1/2MS培养基最适宜;激素NAA和IBA对黄苗影响差异不显著,NAA0.2mg/L较适宜;而附加物质香蕉对黄苗的影响较大,黄苗较严重。     

Prokaryotic Expression of Brucella BAB Gene and Establishment of iELISA

In this study,the genome of Brucella Rev.1 strain was used as a template to amplify the BAB gene sequence,clone it into the prokaryotic expression vector pET-30a(+),obtain the recombinant plasmid pET-30a-BAB,and perform prokaryotic expression on the recombinant plasmid.The indirect ELISA method was constructed by using the expression products detected by Western blotting.The results showed that the BAB gene was successfully cloned and expressed in this experiment,and the purified expression product was analyzed by SDS-PAGE.This study obtained a relatively pure recombinant BAB protein;Western blotting test showed that the expressed protein could react specifically with Brucella sheep positive serum and had good reactogenicity;Using the recombinant BAB protein as the coating antigen,an indirect ELISA method for detecting BAB antibodies was established and optimized.The best determined coating conditions were as follows BAB protein coating amount was 0.25 μg/mL,serum dilution was 1:400;blocking solution was 3% pig-derived gelatin;secondary antibody dilution was 1:6 000;color development time was 10 min.The established method was used to detect 40 clinical sheep serums,and the cut-off value was calculated to be 0.607.That was,when the serum tested had P/N ≥ 1.5 and D_{450 nm} ≥ 0.607,it was judged as positive,when D_{450 nm} ≤ 0.561,it was judged as negative,and when 0.607<D_{450 nm}<0.561,it was judged as a suspect value,and retest was required.Compared with the Huhong plate test and the test tube agglutination test,the positive coincidence rate was 100%,the negative coincidence rate was 71.88%,and the total coincidence rate was 77.5%.     

复方中药提取物在新城疫B_1株免疫中的免疫增强作用

用自制的中药提取物与新城疫Ｂ１株混合后免疫５５日龄鸡，以血凝抑制试验测定新城疫抗体效价。结果表明，５５日龄鸡的中药提取物最佳用量为１．０ｍＬ，试验组鸡ＮＤ抗体效价明显高于对照组。免疫后６０ｄ用Ｆ４８Ｅ８新城疫强毒进行攻击，试验组的保护率达到１００％，而对照组相对较低。