基于加权Voronoi图的林木竞争指数

以福寿山林场25年生的杉木生态公益林为研究对象,通过分析林木竞争指数与胸径生长因子的相关关系,得出二者为负相关,从而确定加权Voronoi图的权重为胸径倒数(W),借助Arcgis软件里的加权Voronoi图工具,通过加权Voronoi图的方法来确定竞争单元,提出基于加权Voronoi图的W_V_Hegyi竞争指数,最后将W_V_Hegyi竞争指数与Hegyi、V_Hegyi竞争指数进行对比分析,结果表明:1) 3种竞争指数与胸径相关性强弱排序为:W_V_HegyiV_HegyiHegyi;2) 3种竞争指数与胸径服从幂函数关系,曲线拟合度排序为:W_V_HegyiV_HegyiHegyi;3) 样地竞争指数平均值排序均为:V_HegyiW_V_HegyiHegyi。通过以上比较分析证明,W_V_Hegyi竞争指数比Hegyi、V_Hegyi竞争指数能更准确地反映林木间的竞争关系。      

解决夏季母猪“饥而不饿”的营养招数

夏季来临，温度是影响猪群健康的第一要素，尤其对于母猪，在高温下其对营养的吸收利用及代谢均会发生改变，导致母猪代谢不畅而不愿采食，这将严重影响猪群健康及繁殖成绩。文章将从营养调节角度分析，并结合生产实践来解决这一问题，以期为猪场夏季母猪健康养殖提供借鉴和参考。     

Sequence Analysis and Prokaryotic Expression of NSP1 Gene of Bovine Rotavirus UK Strain

Rotavirus is a major cause of acute diarrhea in both many kinds of young animals and children under 5 years old.Rotavirus NSP1, a 55 ku RNA binding protein, is the product of gene 5, which can subvert innate immune responses and be one of virulent determinant factors.According to the sequence in GenBank, specific primers targeting to NSP1 gene were designed and the gene was amplified by RT-PCR, following by being cloned into the pET-28a(+) vector.It showed that the full length of NSP1 gene was 1 473 bp, encoding 491 amino acids.The NSP1 shared the highest identity with WC3 strain.The recombinant protein was induced in E.coli Rosetta(DE3) by IPTG and was analyzed by SDS-PAGE and Western blotting.The results revealed that NSP1 recombinant protein existed in the form of inclusion body with the molecular weight of 55 ku.The purified recombinant protein could be recognized by His-tag antibody.This study laid the foundation for further research on the relationship between the intracytoplasmic location of NSP1 protein and its activity.     

增密减氮对弱筋小麦‘宁麦13’产量和品质的影响

为实现弱筋小麦优质稳产,解决当前弱筋小麦存在品质稳定性差的问题。本试验以弱筋小麦‘宁麦13’为试材,结合方差分析等方法研究增密减氮对弱筋小麦的产量、群体质量指标以及籽粒品质的影响。结果表明,在240 kg/hm²施氮水平条件下,随着密度的增加,小麦LAI、干物质积累量均呈先增加后下降的趋势,密度超过240×10⁴/hm²会导致LAI、干物质积累量、产量下降。在240×10⁴/hm²密度条件下,施氮量超过240 kg/hm²会导致小麦叶面积指数、SPAD值、花后干物质积累量和产量下降。适当的增密减氮有利于提高弱筋小麦的优质稳产,而过量增密减氮则会导致小麦产量下降,品质不稳定。为实现产量和品质的最优化,生产上推荐采用种植密度为240×10⁴/hm²,施氮量为180 kg/hm²,氮肥运筹为7:1:2:0的栽培模式。     

草果AtNCED基因克隆、表达和植物表达载体构建

9-顺式环氧类胡萝卜素双加氧酶(9-cis-epoxycarotenoid dioxygenase, NCED)是ABA生物合成的限速酶。诸多研究表明NCED基因表达量的变化与ABA含量显著相关,在种子休眠过程中发挥重要作用。为探究草果AtNCED基因的序列特征和功能,本研究以草果(Amomum tsaoko Crevost et Lemarie)种子转录组为基础,采用RT-PCR技术克隆了一个AtNCED基因。该基因全长2 372 bp,包含一个1 830 bp的开放阅读框(open reading frame, ORF),编码610个氨基酸。生物信息学分析显示,AtNCED基因编码的蛋白在N-末端包含典型的叶绿体转运肽序列,在催化结构域含有4个高度保守的组氨酸残基。AtNCED蛋白与芭蕉科马来西亚蕉同源性较高,与其他单子叶植物处在同一进化分支。实时荧光定量PCR分析结果表明,AtNCED基因表达量随着层积时间的增加逐渐下降,该基因可能正向调控种子休眠,在草果种子休眠诱导与维持中发挥重要作用。进一步地,利用同源重组方法,成功构建了pBI121-AtNCED过表达载体,并转化至农杆菌EHA105。该研究结果为弄清ABA激素信号在种子休眠中的调控作用提供了帮助。     

甘薯耐旱性品种苗期筛选及耐旱性指标研究

为实现甘薯耐旱性品种的快速筛选,本试验采用室内PEG模拟连续干旱法对22个甘薯品种(系)进行了研究。结果表明:济薯21、12057、11001和零9281等品种(系)的根系持水力、根系活力和幼苗成活率较高,具有较强的苗期耐旱性;通过统计分析发现,根系持水力、薯苗质量和含水量、根系活力等可作为重要的苗期耐旱性筛选指标;干旱胁迫下根系的持水能力和采苗后薯苗状况是影响甘薯苗期耐旱性的重要因素。在生产上,选用耐旱性品种和采用壮苗是抵御干旱胁迫的重要措施。     

基于农作制分区的1985—2015年中国小麦生产时空变化

Comparing spatio-temporal variation characteristics of China’s wheat production, yield, sown area and yield-area-contribution in different farming zones in the past 30 years could help improving wheat planting layout and adjusting planting structure. Concentration index, rate of change, moving of gravity and resolution of yield-area-contribution were used to analyze spatio-temporal changes of China’s wheat production and yield-area-contribution based on county wheat production statistics including sown area, production and yield from 1985 to 2015. The wheat sown area decreased obviously in Northeast farming region, Northwest farming region and South farming region and increased rapidly in Huang-Huai-Hai farming region and Yangtze Plain farming region. In Huang-Huai-Hai farming region, the concentration indexes of Haihe Plain farming region, Huang-huai Plain farming region and Fenwei Basin farming region reached to 20.64%, 25.77%, and 21.65% respectively in 2015. Wheat production increased significantly by more than 48 milliontons in Huang-Huai-Hai farming region, and nearly 8 million tons Yangtze Plain farming region but decreased by more than 2.6 million tons in Northeast farming region. In Huang-Huai-Hai farming region, wheat production was concentrated in Haihe Plain farming region, Huang-huai Plain farming region and Yuxi Hill farming region. The average wheat yield continuously improved during the study period, Huang-Huai-Hai farming region and Northwest farming region had the yield increase up to 103.5 kg hm ^-2 and 92.9 kg hm ^-2 each year. Wheat yield in Yuxi Hill farming region, Fenwei Basin farming region and Haihe Plain farming region was relatively high in Huang-Huai-Hai farming region. The yield-reduced area was mainly caused by the decreasing of sown area, while the yield-area-contribution rate was different in yield-increased area. Yield-dominant counties were reduced, area-dominant counties were increased and yield-area-dominant counties were relatively steady. In production increased area, yield-dominant and yield-area-dominant counties were the main types in Huang-Huai-Hai Farming region, area-dominant and yield-area-dominant counties were the main types in Yangtze Plain farming region. Chinese wheat production was increasingly concentrated in Huang-Huai-Hai farming region which has high and rapid increase of wheat yield over the past three decades. Haihe Plain farming region, Huang-huai Plain farming region and Fenwei Basin farming region were the most concentrated areas in Huang-Huai-Hai farming region for wheat production during this period. Wheat yield and sown area jointly promoted the increase of wheat production in Huang-Huai-Hai farming region, wheat sown area was the crucial factor to increase wheat production in Yangtze Plain farming region, especially in the north of Jiangsu, Anhui province and greater part of Xinjiang.