低温胁迫下马铃薯的数字基因表达谱分析

以马铃薯品种合作88为材料,利用数字基因表达谱(DGE)技术,对–2℃低温胁迫处理后的马铃薯叶片c DNA文库进行差异基因表达谱分析。结果表明,有28 505个基因受低温胁迫诱导差异表达,其中上调表达基因13 703个,下调表达基因14 802个。GO功能显著性富集分析表明,DEGs主要涉及信号生物代谢过程、氧化还原过程、能量代谢、次生代谢过程以及催化活性。KEGG富集分析表明,上调表达基因主要富集于苯丙烷、光合作用天线蛋白、类胡萝卜素的生物合成、苯丙氨酸代谢及淀粉与蔗糖代谢途径,而下调表达基因主要富集于植物激素信号转导途径。利用实时荧光定量PCR(q RT-PCR)验证4个DEGs在低温胁迫条件下的差异表达,其结果与DGE分析结果基本一致,证实了DGE测序结果的可靠性。     

盐碱胁迫对紫花苜蓿和草木樨发芽及出苗的影响

为选择适宜在盐碱土上种植的牧草种类,合理开发和利用盐碱地资源,分别进行室内发芽和室外出苗试验,研究盐碱胁迫对紫花苜蓿和草木樨发芽及出苗的影响.结果表明,各个浓度模拟盐碱胁迫均对紫花苜蓿的发芽有抑制作用;50 mmol/L盐溶液和5 mmol/L碱溶液对草木樨发芽有促进作用,150～250 mmol/L盐溶液和5～25 mmol/L碱溶液其发芽有抑制作用.研究同时表明,紫花苜蓿适宜种植于极轻度盐化土壤和极轻～轻度碱化土壤(pH值为8～9,EC为0.2～0.7mS/cm);草木樨则适宜种植于轻度～重度盐化土壤和中度～重度碱化土壤(pH值为9～10,EC为0.7～1.6 mS/cm).     

Evaluation on Tolerance of Montmorillonite as a Mycotoxin Adsorbent

In order to study the tolerance dose of Kunming mice to montmorillonite as a mycotoxin adsorbent in diets,the experiment selected 60 healthy male Kunming mice with initial average body weight of (17±0.12)g,randomly divided them into 5 treatments with two replicates per group and 6 mice per replicate.Five experimental diets were added to 0 (control group),0.5%,1%,2% and 5% montmorillonite,respectively.After 30 days,the growth performance,nutrients apparent digestibility,serum biochemical indices and internal organization were tested to determine the tolerance dose of montmorillonite for Kunming mice.The results showed that the ADFI was significantly increased in 0.5% group (P< 0.05),while there were no significant differences in ADFI and ADG between 1% group and control group (P> 0.05),then the ADFI was significantly decreased with the increasing of montmorillonite supplemental level (P< 0.05).The ADG in 2% group had no significant difference when compared with the control group (P> 0.05),while significantly lower than that in 0.5% and 1% groups (P< 0.05).The apparent digestibility of DM,CP and EE showed no significant effect at any level of montmorillonite (P> 0.05).Serum ALT,AST activities and MDA content were gradually increased with the montmorillonite supplemental level increasing,and that in 1%,2% and 5% groups were significantly higher than that in control group (P< 0.05),but there were no significant differences between 0.5% group and control group (P> 0.05).Tissue sections showed that mice livers were damaged in varying degrees when the montmorillonite supplemental level was 5%.In conclusion,based on a comprehensive consideration of all indexes,it could be seen that the tolerance dose of the tested montmorillonite for Kunming mice was between 0.5% and 2% when mycotoxin contents in diets were in security range.     

ArcPad在营造林外业调查系统中的应用

详细介绍了ArcPad在支持GIS的PDA设备中的应用技术,以营造林外业调查系统为例,详细给出了通过ArcPad结合空间数据库在PDA设备中构建营造林外业调查系统的方法和步骤。     

基于模糊数学评价法优化黑豆百合酸奶配方

以黑豆、百合为主要原料,以感官评定为指标,结合模糊数学综合评定方法对黑豆百合酸奶产品品质的各因素进行了分析。结果表明:影响酸奶品质的因素大小为黑豆乳白砂糖全脂奶粉百合乳;当黑豆乳添加量为70%,百合乳添加量为20%,全脂奶粉添加量为10%,白砂糖添加量为9%时,黑豆百合酸奶产品组织细腻,黏度合适,口感适宜。     

提高轮机专业英语考证通过率的思考

明确轮机专业英语的教学目标;分析船员适任考试中轮机英语合格率不高的原因;提出改进英语教学模式,做好大学英语和专业英语教学间的有机衔接,加强轮机专业英语师资的培训,设立航海类英语教学与语言训练中心等教学改革措施;努力提高轮机英语的教学效果.     

高校图书馆大流通环境下的读者教育与管理探析

大流通运行模式已在高校图书馆普遍运用，其优势非常明显，但存在的问题也比较突出。通过对大流通的内涵、特征及其在大流通环境下存在的问题进行分析，指出了产生问题的主要原因，提出了图书馆加强读者教育与管理的思路。     

我国猕猴桃产业发展现状及对策建议

近年来我国猕猴桃产业发展迅速,产业发展潜力很大,但由于起步较晚、主导品种不明确、管理技术体系落后、质量标准体系不完善等原因,仍然存在诸多问题。主要问题包括(:1)尽管采收面积和产量均位居世界第一位,但单位面积产量低,而且生产总量占水果总量的比例仍然很小(;2)果实品质较差,进口单价始终高于出口单价,进出口贸易逆差明显。笔者通过对国内外猕猴桃生产、贸易数据的统计分析和产业调研,总结产业发展现状,梳理出我国猕猴桃产业存在的主要问题,并分析产生的原因,为促进我国猕猴桃产业健康发展提出对策及建议。     

EZH2-mediated regulation of NF-κB target gene expression in gastric cancer

AIM: To explore the mechanism by which over-expression of enhancer of zeste homolog 2 (EZH2) in a panel of gastric cancer cell lines is involved in tumorigenesis of gastric cancer. METHODS: Real-time PCR and Western blot were employed to examine the mRNA and protein levels of EZH2, respectively. MTS assay, cell migration and soft agar assay were performed to investigate the role of EZH2 in the regulation of stomach cancer behaviors. The effect of EZH2 on NF-κB target gene expression was determined by Luciferase reporter and real-time PCR. Co-immunoprecipitation was used to analyze the interaction of EZH2 and p65 in HEK293T cells. RESULTS: The expression levels of EZH2 were significantly increased in the gastric cancer cells compared with normal gastric epithelial cells. Pharmacological inhibition by DZNep or knockdown of EZH2 significantly compromised AGS and SNU-16 cell activity, cell migration and anchorage-independent cell growth. Moreover, siRNA knockdown of EZH2 impaired NF-κB downstream targets, such as IL-8, CXCL5 and CCL20. In addition, the interaction of EZH2 and p65 was detected. CONCLUSION: EZH2 mediates the growth of gastric cancer cells through the regulation of NF-κB downstream gene expression.     

设施韭菜安全生产关键技术集成

生产绿色、安全、放心韭菜，除了应用优质品种、实施绿色韭菜安全施肥技术外，推广绿色韭菜韭蛆防控新技术是关键。