Regulatory effect of salicylic acid and methyl jasmonate supplementation on ergosterol production in Hericium erinaceus mycelia

We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid(SA) and methyl jasmonic acid(Me JA)supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control(p \ 0.01). Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in H.erinaceus mycelium.     

金中都公园的园林景观与历史文化展示

本文试以北京金中都公园设计和建设过程为例,论述如何将园林景观与历史文化的合理展示相结合。提出设计师的创作和实践应该与建设过程相配合,以匠人精神应对施工阶段的现场完形和设计提升。总结了设计项目从概念规划直至建成,设计师的全过程设计的重要性和必要性。探讨了景观与艺术专业的协同设计在文化表达和景观展示中的叠加效应。提出设计师对场地的挖掘和设计推敲,应该随着项目的施工建设进度并以现场设计为核心,并举例论述了现场调整对提升设计品质的重要作用。以期为公园改造和园林景观设计师的工作实践提供有益的参考和引导。     

漳州市加快发展对台农业交流合作政策选择的研究

随着ECFA的实施,漳台(漳州市、台湾省,下同)农业资源将进一步得到合理配置,漳台农业交流合作的空间和领域将得到更大的拓展。漳州市如何在现有的基础和成效上抓住这些有利时机,突破制约漳台农业进一步合作的瓶颈,努力促进漳台农业合作实现跨越式发展,具有重大的现实意义和深远的历史意义。     

温室黄瓜新品种中农33号的选育

中农33号是以自交系09676和12173为父母本配制的黄瓜一代杂种。植株生长势强,耐低温弱光性好。果实商品性好,颜色深绿、有光泽,腰瓜长约35 cm,瓜粗3.4 cm左右,瓜把长约4.5 cm,心腔较小,果肉为淡绿色。白刺、密,瘤小,无棱。高抗WMV,中抗CGMMV、枯萎病、黑星病。早熟性好,持续结果及丰产优势明显。温室长季节栽培每667 m~2产量高达10 000 kg以上。适宜日光温室和春棚栽培。     

Mycoplasma pneumoniae induces IL-1β production through activating NLRP3 inflammasome by ROS in RAW264.7 cells

AIM: To investigate whether Mycoplasma pneumoniae (Mp)-induced interleukin-1β (IL-1β) production in RAW264.7 cells is through the activation of NLRP3 inflammasome via reactive oxygen species (ROS). ME-THODS: RAW264.7 cells were randomly divided into 3 groups. In normal group, RAW264.7 cells were treated without Mp. In model group, RAW264.7 cells were treated with 1∶ 10 multiplicity of infection (MOI) of Mp. In NAC group, RAW264.7 cells were pretreated with N- acetylcysteine (NAC) at a concentration of 5 mmol/L for 30 min before infection with Mp. The RAW264.7cells were infected with Mp (1∶ 10 MOI) for 4, 8, 16 and 24 h in model group and NAC group, respectively. The intracellular ROS level was analyzed by flow cytometry. The mRNA expressions of NLRP3, ASC and caspase-1 were detected by real-time PCR. The protein levels of NLRP3, ASC and caspase-1 p20 were determined by Western blot. The levels of pro-inflammatory cytokine IL-1β in the supernatant were measured by ELISA. RESULTS: Compared with normal group, the production of ROS were significantly increased at 4, 8, 16 and 24 h after infection, the mRNA expression of NLRP3, ASC and caspase-1 were increased at 8, 16 and 24 h after infection, the protein levels of NLRP3, ASC and caspase-1 p20 were increased at 16 and 24 h after infection, and the releases of IL-1β were increased at 24 h after infection in model group (P<0.01). Compared with the model group, the level of ROS in NAC group decreased, so as the expression of NLRP3, ASC and caspase-1 at mRNA and protein levels and the releases of IL-1β in the supernatant at the corresponding time points. CONCLUSION: Mp may stimulate the ROS production to activate NLRP3 inflammasome in RAW264.7 cells.     

农机车载电子设备用户界面框架设计

介绍了一种农机车载电子设备显示界面框架的设计与实现。基于C语言分析设计了界面框架的数据结构,使用分层的数据结构设计方法实现了多类型数据的显示需要和灵活的界面类型组合方式,以极少的开销实现了多级菜单。将内容数据与操作函数分离,解决了操作函数复用的问题,同时提高了程序的可维护性。提出的新型的界面显示框架实现了多种农业装备常见的界面应用类型,解决了小屏幕嵌入式系统显示界面移植性差问题。     

浙江省食用菌产业发展历史与建议

总结回顾浙江省食用菌发展的历史和取得的成就,指出食用菌是浙江省农业十大主导优势产业之一,食用菌栽培种类全,分布区域集中,规模化生产水平较高;在科学技术进步、农业丰收、技术改造、品牌建设、国家地理标志等方面获得了诸多成果与荣誉,涌现出一大批优秀的行业先锋;最早提出并制定一系列食用菌标准化生产规范,并注重“菌文化”的传承。浙江食用菌面临的主要困难有:发展动力减弱,生产规模下滑;食用菌产业科技支撑不足,缺少自主核心技术;品牌以及消费宣传不够,缺乏正确的饮食引导。提出具体发展建议。     

重庆烟区硅酸盐细菌的分离鉴定及生理生化特性

采用土壤稀释平板法从重庆市彭水县的植烟土壤样品中分离出8株具有解钾能力的细菌,通过测定其对含钾矿石中有效钾的释放能力,筛选出两株具有较强的解钾能力的硅酸盐细菌,运用分子生物学方法对这两株硅酸盐细菌的16~23S rDNA序列进行扩增并鉴定。结果表明,一株细菌属于胶质类芽孢杆菌(Paenibacillus mucilaginosus),另一株细菌属于短短芽孢杆菌(Brevibacillus brevis),并且研究了这两株细菌的生理生化特性。     

Variations of SOC and MBC observed in an incubated brown loam soil managed under different tillage systems for 12 years

To assess changes in organic carbon pools, an incubation experiment was conducted under different temperatures and field moisture capacity (FMC) on a brown loam soil from three tillage practices used for 12 years: no‐till (NT), subsoiling (ST) and conventional tillage (CT). Total microbial respiration was measured for incubated soil with and without the input of straw. Results indicated that soil organic carbon (SOC) and microbial biomass carbon (MBC) under ST, NT and CT was higher in soil with straw input than that without, while the microbial quotient (MQ or MBC: SOC) and metabolic quotient (qCO₂) content under CT followed the opposite trend. Lower temperature, lower moisture and with straw input contributed to the increases in SOC concentration, especially under NT and ST systems. The SOC concentrations under ST, with temperatures of 30 and 35°C after incubation at 55% FMC, were greater than those under CT by 28.4% and 30.6%, respectively. The increase in MBC was highest at 35°C for 55%, 65% and 75% FMC; in soil under ST, MBC was greater than that under CT by 199.3%, 50.7% and 23.8%, respectively. At 30°C, the lower qCO₂ was obtained in soil incubated under NT and ST. The highest MQ among three tillage practices was measured under ST at 55% FMC, NT at 65% FMC and CT at 75% FMC with straw input. These data indicate the benefits of enhancing the MQ; the low FMC was beneficial to ST treatment. Under higher temperature and drought stress conditions, the adaptive capacity of ST and NT is better than that of CT.     

A型流感病毒分型基因芯片在流感病毒各亚型监测中的应用

为验证自行研制的A型流感病毒分型基因芯片方法在实际中的应用效果,了解A型流感病毒各亚型在我国不同地区和不同宿主中的分布情况,对我国20个地区、不同宿主源(鸡、鸭、鹌鹑、鸽子、猪、人等)拭子和组织样品共16 852份进行了检测。利用自行研制的A型流感病毒分型基因芯片方法,对样品进行RNA提取、多重不对称RT-PCR扩增、芯片杂交、洗涤和扫描分析;同时将基因芯片检测阳性的样品,用普通RT-PCR扩增后进行序列测定。结果显示:利用该方法检出阳性样品2 582份,总检出率为15.32%(2 582/16 852),共检测到H1N1、H2N9、H3N2、H4N6、H5N1、H7N1、H7N9、H9N2、H10N5、H16N3等10个亚型,且均与阳性样品测序结果一致。结果表明,该方法可准确检测不同地区、不同宿主中的A型流感病毒不同亚型。同时,该方法能在同一张芯片上准确鉴定A型流感病毒的多种亚型,解决了其他常规方法无法检测已发现和可能发生变异的所有亚型的棘手问题,从而为A型流感诊断和分子流行病学监测提供了一种新技术。