职业大学的新开端新思路

从职业大学的现状和我国社会经济发展的形势出发 ,提出职业大学面向 2 1世纪的发展目标和应有的新开端、新思路 ,并着重对职业大学的人才培养模式、产学合作机制、继续教育、办学形式等进行了带有设想性的分析。     

Effects of salvianolic acid B on neurons during oxygen/glucose deprivation and reintroduction

AIM: To observe the damage induced in the primary cultured rat cortical neurons by oxygen/glucose deprivation and reintroduction, and to investigate the neuroprotective mechanism of salvianolic acid B (SalB). METHODS: Primary cultured rat cortical neurons were randomly divided into the control group, the model group and the SalB group. The cell model was established by oxygen/glucose deprivation for 3 h followed oxygen/glucose reintroduction for 24 h. The cortical neurons viability was determined by MTT assay. The leakage rate of lactate dehydrogenase (LDH) was measured by chromatometry. The mitochondrial membrane potentials (MMP) and the apoptosis rate were quantitatively analyzed by flow cytometry. The cytosolic free calcium was assessed using LSCM. The morphologic changes of neuronal nuclei were observed by Hoechst 33342 fluorescence staining. RESULTS: Compared to the model group, the cortical neurons viability, the survival rate and the fluorescence value of MMP in the SalB group were obviously increased (P<0.05,P<0.01). In addition, in the SalB group, the leakage rate of LDH, the fluorescence intensity of cytosolic free calcium and the apoptosis rate were significantly lower than those in the model group (P<0.01). CONCLUSION: The neuroprotective mechanism of SalB in the oxygen/glucose deprivation and reintroduction neurons would be due to the fact that SalB maintains the MMP and the calcium homeostasis.     

不同钾肥品种和用量对大蒜产量、品质和养分吸收利用的影响

以河南中牟为供试地点,对不同钾肥品种和罗布泊钾肥不同用量在大蒜上的应用效果进行了研究,以了解钾肥在大蒜上的应用效果.结果表明:不同钾肥处理都可以增加大蒜蒜薹、蒜头鲜重,蒜头瓣数、蒜头直径、茎周长,可以促进大蒜对氯素的吸收,提高Vc、蛋白质含量,但不同钾肥种类和用量袁现不同.利用回归方程求得罗布泊K2SO4的最佳经济效益施用量为253.5kg/hm2时,最大产值为33 495.5元/hm2.     

不同方式接种绿僵菌后松墨天牛带菌量及致死率的比较

对松墨天牛成虫采用爬行、跗节、喷雾3种方式接种金龟子绿僵菌MaYTTR-04后的带菌量和死亡率进行了比较试验,结果表明:不同接种方式松墨天牛体的带菌量间差异极显著(F=79.179,P=0.0001),其中爬行接种的接种量最大,达到(5.60±0.49)×106孢子.成虫-1,是跗节接种的4倍多;接种后15 d,各处理天牛累计死亡率均达100%;模拟各处理毒力回归方程发现,爬行接种的方程的斜率最大,说明处理后天牛在致死中时(LT50)后期死亡速率较快,爬行、喷雾、跗节接种的LT50分别为5.3 d、6.8 d和6.9 d。综合分析表明,3种方式接种绿僵菌后松墨天牛的致死率非常高,其中爬行接种效果最好。可为松墨天牛的生物防治提供有效的施菌技术。     

汽油机曲轴圆角和下沉量的研究

以某汽油机曲轴为载体,计算分析了曲轴的静态性能,并改变圆角半径和圆角下沉量的大小,分析对曲轴强度的影响,从而为圆角滚压工艺选取曲轴圆角参数奠定理论基础。     

利用SQL制作森林资源统计表的方法

介绍利用SQL在SQL Server中制作分类汇总表和交叉统计表的技术，结合常用的森林资源统计表，分析如何在SQL Server中制作静态列交叉统计表和动态列交叉统计表，以实例介绍制作森林资源统计表的方法．并对SQL以及SQL中的SELECT作了简单的介绍，分析了利用SQL制作统计表的优势．     

滇中地区花椰菜引种观察试验初报

于2006年秋,引进51个花椰菜品种,在昆明市海拔1 920 m的试验地安排花椰菜引种观察试验,试验结果表明,太白、一代神良100天、赛白80、玛瑞亚和白阳5个品种商品性优,产量性状也较理想,抗病性较好,具有良好的推广应用前景,适宜在滇中地区种植,建议参加2007年的品种比较试验.     

Jagged1 knocking down in endothelial cells accelerates PDGF induced proliferation and migration of vascular smooth muscle cells in rats

AIM: To investigate the effect of Jagged1 expression in endothelial cells (EC) on platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMC) in rat.METHODS: Rat aorta EC was inoculated in the lower chamber and VSMC were in the upper chamber of the cell coculture system. Three groups were divided: control, sicontrol and siJagged1. The EC Jagged1 protein expression was assayed by Western blotting to evaluate small RNA interfering (RNAi) efficiency. After the cells were cocultured with PDGF for 24 h, the proliferation and migration of VSMC were respectively evaluated by ［3H］-TdR incorporation and migrating cells counting. Protein expression of α-SM-actin in VSMC was assayed by Western blotting. RESULTS: The Jagged1 protein expression in EC was significantly lower in siJagged1 group than that in control group (0.26±0.02 vs 0.67±0.02, P<0.05), and no statistic significance was observed between control and sicontrol groups. The VSMC ［3H］-TdR incorporation and migration were higher in PDGF +siJagged1 group than those in PDGF group {［3H］-TdR incorporation (23 074±2 702) counts·min-1·well-1 vs (16 442±1 803)counts·min-1·well-1, n=5, P<0.05; migration (27±4) cells/field vs (15±3)cells/field, n=5, P<0.05}. The α-SM-actin protein in VSMC was lower in PDGF + siJagged1 group than that in PDGF group (0.25±0.06 vs 0.49±0.04, n=3, P<0.05).CONCLUSION: Jagged1 knock down in rat EC accelerates PDGF induced proliferation and migration of VSMC. These results suggest that Jagged1 expression in EC plays an important role in maintaining VSMC contract phenotype and inhibiting VSMC overgrowth after arterial injury.