PEG引发三倍体无籽西瓜种子萌芽研究初报

为了探索一条能大批量处理无籽西瓜种子,有效提高种子活力的途径,应用水势0、-0.2、-0.6和-1.2MPa的聚乙二醇(PEG-6000)溶液对三倍体无籽西瓜种子进行3、6、9、12h引发处理,并与常规萌发条件下的种子萌芽情况进行比较分析。结果表明:(1)各种水势的PEG-6000溶液引发对无籽西瓜种子发芽各项指标均没有显著影响。(2)3h引发处理相对于其他引发时间处理,能最大程度地提高无籽西瓜种子发芽能力,在发芽率、发芽势、发芽指数和活力指数的影响上均存在明显差异,这与常规生产上无籽西瓜浸种时间结果一致。     

高产优质水稻新品种9香优007的选育

9香优007系铜仁鑫天地农业发展有限公司利用自育恢复系铜恢007与自育不育系9香A杂交选育的新品种.主要特点是:米质优、抗性强、丰产稳产性好.具有较好的推广前景.     

粮食污染物残留免疫分析技术研究与示范

随着人们对粮食安全问题的重视,政府部门不断加强对粮食安全的监测工作。针对粮食生产、加工、储运过程中的化学污染物安全性问题,研究粮食污染物快速监测技术,研究污染物免疫分析方法通过利用抗原、抗体的特异性反应来检测农药含量,设计并筛选吡虫啉、多溴联苯醚、功夫菊酯、氰戊菊酯4种半抗原,制备相关高特异性的抗体,分析吡虫啉、多溴联苯醚、功夫菊酯与气相色谱法(GC)相比,稻谷、大米、玉米样品通过相关性分析均高度相关,操作简便、快速。     

Dexmedetomidine attenuates acute alcoholic hepatic injury in mice

AIM: To investigate the effects of dexmedetomidine (DEX) on acute alcoholic hepatic injury in mice and to explore the possible mechanisms. METHODS: Kunming mice (n=50) were randomly divided into 5 groups (n=10): normal saline control (NS) group, acute alcoholic hepatic injury model (E) group, low-dose (10 μg/kg) DEX (E+L) group, medium-dose (50 μg/kg) DEX (E+M) group and high-dose (100 μg/kg) DEX (E+H) group. The animals were sacrificed at 6 h after gavage of alcohol or normal saline. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were measured. The livers were removed for evaluation of histological characteristics and determining the content of tumor necrosis factor-α (TNF-α) amd interleukin-1β (IL-1β) in the liver tissues by ELISA. The expression levels of cytochrome P450 2E1 (CYP2E1) and nuclear factor-κB (NF-κB) in the liver tissues were evaluated by Western blot. RESULTS: Compared with NS group, the levels of ALT, AST and TG were obviously increased in E group, which were obviously decreased in E+M and E+H groups. Compared with NS group, the levels of TNF-α, IL-1β and MDA were obviously increase in E group, which were obviously decreased in E+M and E+H groups. Compared with NS group, the activity of SOD and the content of GSH were obviously decreased in E group, which were obviously increased in E+M and E+H groups. Compared with NS group, the expression of CYP2E1 and NF-κB was obviously increase in E group, which was obviously decreased in E+M and E+H groups. Compared with NS group, ethanol induced marked liver histological injury, which was less pronounced in E+M and E+H groups. CONCLUSION: DEX has a protective effect on mouse liver with acute alcoholic injury by the involvement in the processes of antioxidation and antiinflammation, and its mechanism may be associated with the inhibition of CYP2E1 and NF-κB expression.     

水肥一体化中水肥混合效果数值模拟研究

为研究水肥一体化技术中的水肥混合特性,以流体力学中的两相流理论为基础,针对水肥混合过程中混合时间、混合区间长度、流速及管径对其混合效果的影响进行相应的数值模拟研究。结果表明,混合时间及混合区间长度越长有利于提高水肥混合效果及最终水肥混合均匀性;水肥流速比较低时管内水肥混合的均匀性较好,且水肥流速越大则其混合效果及均匀性越好;水肥流速较低时输水管及输肥管的管径比对水肥混合均匀性的影响较大,两者管径比越小则混合效果越好,随着流速的提高两者管径比对其混合效果的影响逐渐减弱。     

对贫困乡镇农机管理服务的探讨

随着我国社会经济的发展,贫困乡镇农业机械化进程逐年提高,相应地贫困乡镇农机管理服务的地位也就愈加突出,对农机管理服务者的素质要求自然也是越来越高.但是现在贫困乡镇农业机械化生产过程中仍然存在许多问题.     

城口县蜜源植物资源分析

通过对城口县辖7镇17乡的蜜源植物进行调查,摸清了该地区蜜源植物的种类、数量及分布,并分析了现有蜜源植物的开发和利用现状,为开发和利用城口县宝贵植物基因库,制定该县蜜粉源植物保护与开发方案奠定基础。调查发现该县蜜、粉源植物共有78科、173属、228种,可以作为主要蜜源植物的达24种。     

新疆加工型辣椒花药培养技术研究

以6个新疆加工型辣椒品种207、208、64、66、67、HT113为试材,以MSB培养基为基本培养基,附加不同植物激素浓度的NAA、2,4-D、TDZ、KT进行花药培养,研究不同植物激素处理对加工型辣椒花药愈伤组织和胚状体诱导的影响,以建立适应于新疆加工型辣椒的花药培养技术。结果表明:不同基因型辣椒的愈伤组织诱导率和出胚率均不同,愈伤组织诱导率为4.98%~29.65%,胚状体诱导率为0~6.88%。0.5 mg·L~(-1) 2,4-D+0.1mg·L~(-1) TDZ和0.5mg·L~(-1) NAA+0.1mg·L~(-1) KT分别是愈伤组织诱导和胚状体诱导最适宜的激素组合;在新疆特有的气候条件和种植模式下,7月中旬前的花药接种培养最为适宜;胚状体继续发育成熟后,通过练苗移栽获得了56株单倍体植株。     

SSR分子标记实验操作注意事项

SSR分子标记已经得到普遍应用,初学者如不能很好地掌握实验操作要点,就难以在较短时间内成功地获得试验结果.本文分别介绍了DNA提取、PCR扩增和电泳检测PCR扩增产物等实验过程中容易被忽视的注意事项,以期为普及SSR分子标记实验技术服务.     

菜用甘薯新品种薯绿1号的选育

薯绿1号(原名徐菜薯1号)是以台农71为母本,以广薯菜2号为父本,通过定向有性杂交选育而成的菜用甘薯专用新品种。株型半直立,分枝多,叶片心形,顶叶黄绿色,叶基色和茎色均为绿色。薯块纺锤形,白皮白肉。茎尖无茸毛,烫后颜色翠绿至绿色,无苦涩味,微甜,有滑腻感,食味好。高抗茎线虫病,抗蔓割病。在国家区域试验3个月时间内每667m2产量1800kg左右,如在保护地条件下可周年生产,每667m2产量在5000kg以上。适合江苏、山东、河南、浙江、四川、广东、福建、海南等适宜地区作叶菜种植。