水氮耦合对滁菊产量和品质的影响

采用二因素二次回归旋转组合设计,以盆栽试验方式研究了水、氮因子对滁菊产量和品质的影响,探讨了滁菊人工栽培适宜的水氮管理模式。研究结果表明,水氮耦合对滁菊花产量、叶片水提总黄酮和氯原酸含量、滁菊花水提总黄酮和氯原酸含量均有极显著影响,水氮间存在显著交互作用。氮肥因子对滁菊花产量、总黄酮含量、氯原酸含量的影响大于水分因子。当土壤水分水平为0.284 1,氮肥水平为0.403 7时,即田间持水量80%,氮肥用量0.257g/kg时,滁菊花产量最高,达18.09g/株;当土壤水分和氮肥用量均为-1.414水平时,即田间持水量50%,氮肥用量为0g/kg时,滁菊花总黄酮含量和氯原酸含量最大。从滁菊花产量、品质角度综合考虑,滁菊生育前期水氮管理以中水中氮较为适宜,滁菊现蕾开花期以低水低氮较为适宜。     

油菜机械收获配套农艺技术研究 Ⅰ 不同油菜品种机械收获损失的差异

本文系统地研究了四个油菜品种在机械收获过程中各部位损失量的差异。结果表明：四个油菜品种机械收获损失主要发生在脱粒和清选过程中,占总损失量的80%左右;抗裂角性强的油菜品种可减少自然脱落和割台的损失,但增加了脱粒过程的损失;高产品种虽然机械化收获的损失量最高,但总收获损失率却最低。     

河北省紫花苜蓿草生产技术规程

为了规范河北省紫花苜蓿的生产,促进河北省畜牧业的可持续发展,依据国家、行业标准,结合河北省紫花苜蓿生产实际情况,对紫花苜蓿生产的环境条件、播种、田间管理、病虫害防治和收获贮存等提出了具体要求。该规程对河北省紫花苜蓿生产具有重要的现实指导意义。     

不同铵硝比对菠菜有机酸和淀粉含量的影响

【目的】研究不同铵硝比的氮素营养对于菠菜有机酸和淀粉含量的影响。【方法】采用营养液栽培菠菜的方法,营养液中的氮素形态设置硝态氮和铵态氮以一定比例（100﹕0、75﹕25、50﹕50、25﹕75 和 0﹕100）配合。【结果】随着铵硝比的下降,（1）菠菜茎叶丙酮酸、柠檬酸、α-酮戊二酸、琥珀酸、延胡索酸和苹果酸等6种有机酸含量以及淀粉含量都呈上升趋势;同时,有机酸含量与营养液中初始硝态氮浓度之间呈显著的线性正相关关系;（2）菠菜根系丙酮酸、柠檬酸、琥珀酸和苹果酸含量以及淀粉含量也呈上升趋势,但延胡索酸含量的变化不明显;（3）除了柠檬酸外,菠菜根系其它5种有机酸含量与其茎叶中相应有机酸含量的比值都呈现下降的趋势;在全硝营养条件下,根系中只有琥珀酸和延胡索酸的含量低于其在茎叶中的含量;而在全铵营养时,菠菜根系中上述5种有机酸的含量均明显高于茎叶中的相应有机酸的含量。因此,与根系相比,菠菜茎叶中5种有机酸含量随营养液中硝态氮比例增加而增加的幅度更明显。而随着营养液中硝态氮比例的增加,菠菜根系柠檬酸含量与其茎叶柠檬酸含量的比值逐渐升高,则说明根系柠檬酸含量增加的幅度要大于茎叶柠檬酸含量;（4）菠菜的茎叶和根系中的淀粉含量都呈现下降的趋势,且与营养液中硝态氮浓度之间均呈现为显著的负相关关系。【结论】随着营养液中硝态氮比例的增加,菠菜茎叶和根系有机酸代谢均表现为增强的趋势。     

海门山羊3个基因位点遗传多态性与产羔数的相关分析

对海门山羊群体3个基因位点遗传多态性与产羔数的关系进行了分析,结果表明,在海门山羊群体中,PRLR基因内含子I有2种基因型分别为从型和AB型,PRLR基因内含子Ⅱ有3种基因型分别为CC型、CD型和DD型,FSHR基因外显子10没有多态性;PRLR基因2个多态基因位点基因型与产仔数的最小二乘分析和多重比较表明,PRLR基因内含子I 2种基因型对海门山羊产羔数的影响差异不显著(P>0.05),而PRLR基因内含子Ⅱ 3种基因型对海门山羊产羔数的影响达到了极显著水平(P>0.01).     

Inheritance and QTL Mapping of Salt Tolerance in Rice

An F2 population derived from the cross between Jiucaiqing (japonica) and IR36 (indica) was used to analyze the inheritance of salt tolerance in rice by genetic model of major-genes plus polygenes, and to map the corresponding QTLs by SSR molecular markers. Rice plants of P1, P2, F1 and F2 at 5- to 6- leaf stage were treated under 140 mmol/L NaCI for 10 days. Three indices representing the ability of salt tolerance of rice seedlings were measured, including salt tolerance rating (STR), Na^ /K^ ratio in roots and dry matter weight of shoots (DWS). STR, Na^ /K^ and DWS were all controlled by two major genes with modification by polygenes. Heritability of these traits from major genes was 17.8, 53.3 and 52.3%, respectively. The linkage map constructed by 62 SSR molecular markers covered a total length of about 1 142 cM. There were three QTLs detected for STR located on chromosome 1, 5 and 9, two QTLs for DWS on chromosomes 8 and 9, and two QTLs for Na^ /K^ on chromosomes 2 and 6, one on each chromosome respectively. Single QTL accounted for 6.7 to 19.3% of phenotypic variation. Identification method of salt tolerance in rice and breeding of rice varieties with salt tolerance based on molecular markers assisted selection had been discussed.     

抗猫泛白细胞减少症病毒单克隆抗体的制备及其生物学特性鉴定

用 PEG60 0 0沉淀和蔗糖密度梯度离心从细胞培养物中纯化猫泛白细胞减少症病毒 ( FPV) ,以纯化FPV免疫 BALB/c小鼠 ,运用淋巴细胞杂交瘤技术 ,获得了 4株抗 FPV的特异性单克隆抗体 ( Mc Ab)。其腹水 Mc Ab的 ELISA效价在 1 0 - 4～ 1 0 - 5之间 ,其中 1株具有血凝抑制能力。经 ELISA阻断试验及 ELISA交叉反应性试验测定 ,这 4株 Mc Ab可与 FPV、犬细小病毒 ( CPV)和水貂肠炎病毒 ( MEV)呈特异性反应 ,因此可作为检测 FPV、CPV和 MEV共同抗原的通用试剂     

黄土高原典型草原植被及土壤化学计量对降水变化的响应

研究植被和土壤生态化学计量及其耦合关系,是解析气候变化背景下草地植被养分动态平衡和能量流动的重要途径.本研究以宁夏固原云雾山典型草原为研究对象,采用控雨-滴灌装置模拟3个降水梯度(自然降水的50％,100％和 150％),分析了植物叶片、根系、凋落物及土壤的碳(Carbon,C)、氮(Nitrogen,N)、磷(Pho...     

Jagged1 knocking down in endothelial cells accelerates PDGF induced proliferation and migration of vascular smooth muscle cells in rats

AIM: To investigate the effect of Jagged1 expression in endothelial cells (EC) on platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMC) in rat.METHODS: Rat aorta EC was inoculated in the lower chamber and VSMC were in the upper chamber of the cell coculture system. Three groups were divided: control, sicontrol and siJagged1. The EC Jagged1 protein expression was assayed by Western blotting to evaluate small RNA interfering (RNAi) efficiency. After the cells were cocultured with PDGF for 24 h, the proliferation and migration of VSMC were respectively evaluated by ［3H］-TdR incorporation and migrating cells counting. Protein expression of α-SM-actin in VSMC was assayed by Western blotting. RESULTS: The Jagged1 protein expression in EC was significantly lower in siJagged1 group than that in control group (0.26±0.02 vs 0.67±0.02, P<0.05), and no statistic significance was observed between control and sicontrol groups. The VSMC ［3H］-TdR incorporation and migration were higher in PDGF +siJagged1 group than those in PDGF group {［3H］-TdR incorporation (23 074±2 702) counts·min-1·well-1 vs (16 442±1 803)counts·min-1·well-1, n=5, P<0.05; migration (27±4) cells/field vs (15±3)cells/field, n=5, P<0.05}. The α-SM-actin protein in VSMC was lower in PDGF + siJagged1 group than that in PDGF group (0.25±0.06 vs 0.49±0.04, n=3, P<0.05).CONCLUSION: Jagged1 knock down in rat EC accelerates PDGF induced proliferation and migration of VSMC. These results suggest that Jagged1 expression in EC plays an important role in maintaining VSMC contract phenotype and inhibiting VSMC overgrowth after arterial injury.     

中药复方制剂补中益气散对母猪生产性能的影响

目的试验以中兽医理论为基础,运用中药复方制剂补中益气散对怀孕后期母猪进行产前调理,通过对其生产性能的影响展开研究,旨在为猪饲料中添加使用安全、绿色、有机的中药复方制剂奠定基础。方法将50头预产期相近的母猪,随机分为2组,每组各25头。第1组为对照组,饲喂常规日粮,第2组为试验组,饲料中按1.5%添加中药复方制剂补中益气散,试验组每头母猪添加中药量每天45 g。母猪产仔当天停止给药。母猪生产后,对产仔数、产活仔数、产健仔数、死胎数、弱仔数、木乃伊胎数,以及母猪有无流产等情况进行记录。23 d断奶后对仔猪的腹泻和死亡进行记录。结果①试验组有1头母猪产前出现发烧和食欲不振的情况;对照组有5头出现相同症状;②母猪生产后,对照组产下仔猪227头,死仔12头;试验组产下仔猪254头,死仔9头;③对照组平均窝产活仔数9.08头;试验组平均窝产活仔数10.16头;④对照组发生腹泻的仔猪74头,腹泻率为32.6%;试验组发生腹泻的仔猪10头,腹泻率为3.9%;⑤断奶后对照组窝均健仔数为7.16头;试验组窝均健仔数为9.28头。⑥对照组最后存活179头,存活率为78.9%;试验组最后存活232头,存活率为91.3%。结论①通过运用中药复方制剂补中益气散对母猪进行产前调理,能够有效预防哺乳期仔猪腹泻的发生。②在怀孕期母猪饲料中添加中药复方制剂补中益气散能够提高母猪免疫功能和生产性能。