伊氏锥虫多种克隆群体的采集及血清学检测

针对伊氏锥虫抗原变异，国内外学者对抗原变异规律进行了研究，对其抗原变异程序、变异数量、变异频率等有了一定的了解，在此基础上进行免疫学研究进而寻求防治锥虫病的免疫学手段。但多局限于某种动物个体感染锥虫后的一小段有限的时间进行，所获得的抗原变异型数量较少，因而很难澄清如：一个虫株究竟能有多少变异机会，是以怎样的程序变异的等问题。     

N、P、K对杉木幼苗生长量的影响

开展了施用N、P、K肥对杉木幼苗期生长量影响的研究。结果表明:施肥能显著促进杉木幼苗苗高和地径的生长,但需N、P、K肥配合施,单施N肥的效果较N、P、K配施的效果差,单施N肥和N、P、K肥配施的处理间达到极显著差异。不同施肥处理对杉木苗木的苗高和地径生长的影响也达到极显著差异。此试验最为合理的施肥配方是:N 1.5 g、P 5 g、K 1.5 g,苗高比对照高出38.8%,地径比对照高出46%,可在生产中推广使用。表现最差的施肥配方是N 1.5 g,苗高比对照高出17.4%,地径比对照高出20.0%。     

贵州省鸟类新纪录——栗树鸭

2020年4月14日,在贵州省贵阳阿哈湖国家湿地公园保育区内(106°39′0.13″E,26°30′28.46″N,1107 m)开展鸟类监测,于浅水沼泽区拍摄到2只雁鸭类,经鉴定为栗树鸭(Dendrocygna javanica)。通过查阅相关文献,该种在贵州省的分布尚未记录过,属贵州省鸟类新纪录。本文对发现地概况、形态特征、生境特征和分布现状以及对该物种的保护进行了简要讨论。     

感应热处理工艺对拖拉机轴类零件扭转强度的影响

对驱动轮工艺轴进行了7种工艺方案的感应热处理表面强化,然后对工艺轴进行扭转破坏试验。通过对驱动轮工艺轴扭转断裂的研究,为驱动轮轴及承受扭力的轴类零件结构设计、工艺流程设计和热处理技术要求提供指导,从而提高其扭转疲劳强度和使用寿命。     

HPLC法测定咪松酚乳膏中硝酸咪康唑、醋酸地塞米松及丹皮酚的含量

采用超声波及冰浴法对咪松酚乳膏进行前处理，以反相高效液相色谱法同时测定了咪松酚乳膏中硝酸咪康唑、醋酸地塞米松和丹皮酚的含量.硝酸咪康唑线性范围为40.5~648.0 μg/mL，r=0.999 1;醋酸地塞米松线性范围为3.05~48.8 μg/mL, r=0.999 8；丹皮酚线性范围为20.5~328.0 μg/mL, r=0.999 4；硝酸咪康唑、醋酸地塞米松及丹皮酚的平均回收率分别为：100.44±1.25(%)， 98.46±1.04(%) 和98.24±1.29(%).     

煤矸石合成P型分子筛的研究

以除铁以后的煤矸石为主要原料,经煅烧和碱熔后,再配料进行水热晶化,得到了P型分子筛。运用正交试验,获得了最优工艺条件为：n（SiO2）/n（Al2O3）=3.3,n（Na2O）/n（SiO2）=1.3,n（H2O）/n（Na2O）=60,在60℃老化3 h、93℃晶化7 h。产物经XRD及SEM检测,为纯净的P型分子筛。     

异花玉兰的冬态特征观测

对异花玉兰的冬芽种类、拟花蕾、分枝习性及成枝规律等因子进行了观测,并对冬芽和拟花蕾进行解剖,结果表明:1当年生枝上有休眠芽、叶芽和拟花蕾3种;2叶芽与拟花蕾外均包被着3~4层芽鳞状托叶,最外层薄革质,外面密被短柔毛,内层纸质-膜质,外面疏被长柔毛;3分枝习性基本为预生分枝呈单阶无歧生长规律。     

Effects of bFGF on brain edema,nerve function injury and autophagy related protein in rats with traumatic brain injury

AIM:To study the effects of basic fibroblast growth factor (bFGF) on brain edema, nerve function damage and autophagy related proteins in rats with head injury. METHODS:The rat model of craniocerebral injury (CI) was constructed. The rats were divided into control group, CI group, and low-, middle-and high-dose bFGF groups (n=10). The CI model was established in CI group, while the rats in control group were not given epidural impact. The rats in low-dose, middle-dose and high-dose bFGF groups were given bFGF at 2, 4 and 6 μg, respectively, by intraperitoneal injection after 30 min. The neurological function in the rats was evaluated by improved neurological function scoring. The rat brain tissues were taken, and the water content was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in the brain tissue were measured by ELISA. The malondialdehyde (MDA) content was analyzed by thiobarbituric acid method. The activity of superoxide dismutase (SOD) was examined by WST-8 assay. The glutathine peroxidase (GSH-Px) activity was detected by colorimetric method. The protein levels of autophagy related proteins LC3-Ⅱ and beclin-1 in the brain tissues were determined by Western blot. RESULTS:The neurological function score was increased significantly of the rats in CI group. The rat model of craniocerebral injury was successfully constructed. Neurological function scores in the rats in low-dose, middle-dose and high-dose bFGF groups were reduced, the water content of the brain tissue was also reduced (P<0.05). The levels of TNF-α, IL-6 and IL-1 β were decreased in the brain tissues (P<0.05), the content of MDA was declined (P<0.05), the activities of SOD and GSH-Px were increased (P<0.05), the protein levels of LC3-Ⅱ and beclin-1 were decreased, compared with the untreated rats in CI group (P<0.05). CONCLUSION:bFGF improves the nerve function of the rats with craniocerebral injury, reduces the water content of the brain tissue, reduces the expression of autophagic protein LC3-Ⅱ and beclin-1.The mechanism is related to the inhibition of inflammatory reaction and oxidative damage.     

天山野苹果林苹果小吉丁虫生真菌调查

以天山野苹果林苹果小吉丁虫为调查对象,采用野外调查、采样和室内分离、鉴定相结合的方法,研究了天山野苹果林苹果小吉丁虫的危害程度及其虫生真菌的种类。结果表明:新源县苹果小吉丁虫虫口密度为0.76头·m~(-1),巩留县为2.72头·m~(-1);导致苹果小吉丁虫死亡的原因排序为物理致死天敌寄生真菌侵染其它;从56份样本中分离得到41株真菌,隶属于4目7属,其中曲霉属(Aspergillus)和链格孢属(Alternaria)真菌数量最多;不同季节样本中都分离到了虫生真菌,夏季得到的真菌种类和数量最多。天山野苹果林苹果小吉丁虫中存在一定数量的虫生真菌,应将虫生真菌作为苹果小吉丁虫生物防治的新方法,进行更加深入的研究。     

90年来中共历代中央领导集体政治交接简评

中国共产党成立90年来一共形成了四代成熟而稳定的中央领导集体。其中,第一、二代领导集体围绕最高领导权力的交接不甚成功,但从总体上讲,四代领导集体之间基本实现了正常的交接,并不断走向制度化。这是党自身能够健康发展,党领导的革命和建设事业兴旺发达的根本保证。