重组hIL-2表达载体的构建及在毕赤酵母中的表达

本试验根据hIL-2氨基酸序列、三维结构及其与受体IL-2R结合机制,设计并合成IL-2Rα亚基结合位点修饰性hIL-2基因片段。合成的修饰性hIL-2基因序列被插入真核表达载体pPIC9K,并转化入毕赤酵母基因组中。经筛选得到阳性重组子后,通过诱导培养得到了表达产物。对表达产物进行Elisa初步测定,结果成功表达出重组hIL-2,表达水平达0.93g/L。本项研究为进一步开展IL-2Rα结合位点修饰性hIL-2的生物活性研究奠定了基础。     

银狐的繁殖年龄和配种日期对繁殖性能的影响

本试验通过对816只母银狐的1 195个繁殖记录和51只公狐的211个繁殖记录进行了整理,统计分析了母银狐配种日期及公、母银狐年龄对主要繁殖性能的影响。结果表明:在唐山地区,母银狐2月29日前发情配种,产胎率和胎断奶成活仔兽数较高,而3月16日以后发情配种,其繁殖效率大大降低;1岁和5岁公银狐比2到4岁公银狐的配种或采精次数低,但1岁公银狐所配母银狐的平均受胎率和胎断奶成活仔兽数均高于其它年龄公银狐所配母银狐。     

威海无花果炭疽病病原鉴定及杀菌剂毒力测定

从2011年山东威海地区的无花果秋果果实黑色病斑上分离到1种炭疽病菌。根据病菌的病原形态、培养特性和致病性,鉴定该病菌为胶孢炭疽菌(Collelotrichum gloeosporioides Penz.)。选用7种杀菌剂进行毒力试验,结果表明,对无花果炭疽菌菌丝生长有较强抑制作用的药剂是氟硅唑、戊唑醇和苯醚甲环唑,其EC50分别为0.0257、0.1830、0.1879μg/mL。     

《山西农业科学》2007—2011年载文与引文情况分析

为了解《山西农业科学》的学术水平和载文质量。以《山西农业科学》2007年—2011年刊发的论文为统计样本,采用文献计量法对其载文、引文及作者情况进行统计分析。结果表明,该刊5年间共出版60期,刊载论文1 961篇,其中,基金论文889篇,基金论文占比平均为45.33%;发文的引文总量为16 309篇,篇均引文为8.32篇;引文语种外文占总引文量的21.29%,略高于该刊2003年—2005年的21.20%;普赖斯指数2007年—2011年呈逐年增加趋势;该刊所载论文第一作者属山西省省外的有413篇,占20.89%,且从2007年—2011年其所占的比例呈逐年递增趋势。该刊的各项指标都有了较大的提高,但还需要从自身质量抓起,并加强多渠道的宣传,为期刊的科学发展而努力。     

过热蒸汽膨化番茄的预干燥条件及干燥模型

热风干燥温度对经甘油溶液处理的番茄的干燥特性、色泽以及过热蒸汽膨化后番茄的比容和复水性均有较大影响,过热蒸汽膨化的预干燥温度为70℃时,干燥速率较大,预干燥番茄色泽好,膨化充分,且复水性好.建立的基于Page方程的预干燥模型可以有效模拟和预测不同热风干燥温度条件下任意时刻番茄的干燥速率及含水率.     

荒漠草原不同地形条件下土壤水分和地上生物量的时空分异

运用方差分析、多重比较、Pearson相关系数及克里格插值方法,在时间和空间尺度上分析了荒漠草原地形因素对土壤水分及草地生物量异质性的影响以及土壤水分和生物量的关系。结果表明：荒漠草原地区土壤水分垂直分布规律明显,土壤水分的变异系数在0～20 cm最小、60～80 cm最大;地上生物量在整个生长季的空间异质性较大。3种地形条件下土壤水分含量及时空分布格局各不相同,说明地形是土壤水分异质性的关键影响因子;地形对植物的生长动态规律影响很大,却对地上生物量的空间分异影响较小。不同生育期,土壤水分与地上生物量的关系不同,生长初期地上生物量与60～100 cm土壤水分显著负相关,说明生长初期的土壤水分是植物生长的关键影响因子,并且植物生长耗水主要来自60～100 cm土层;生长旺期和末期的生物量与土壤含水量相关性较弱,这可能与干旱区植物耐旱器官的建成有关。
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捕食螨的抗药性研究进展

抗药性捕食螨的应用是IPM中生防和化防相结合的重要措施之一。本文综述了农药对捕食螨的影响、捕食螨抗药性现状、抗药性机制、抗药性监测方法、交互抗性及遗传改良等,并介绍了抗药性捕食螨的应用现状及前景,旨在充分保护和利用捕食螨,使生物防治与化学防治有机结合。     

A malware analysis method based on symbolic execution tree

In the malware analysis, it is a common method to monitor malware dynamically in a virtual environment. However, with so many branches of executable pathes, path explosion problem will probably occur, leaving some executable pathes uncovered, and hence harming the comprehensiveness of analysis. To solve this problem, we propose a malware analysis method based on symbolic execution tree. This method introduces sinknode and solves the execution of malicious code path by constructing the symbolic execution tree, so improves the analysis of comprehensive. Experiments to analyze the samples of malware show that the method can enhance the efficiency of the analysis with lower time complexity.     

The study on EMI principle and suppression method of vehicle ignition system

Vehicle ignition system is the main electromagnetic interference source. Electromagnetic radiation produced by ignition system will produce the interference to electromagnetic environment around. It is necessary to study the electromagnetic interference (EMI) principle, noise paths and suppression method. We analyze the electromagnetic interference principle of ignition system, and harness trouble-shooting related to electromagnetic interference is also tested. Results show that the spark discharge between spark plug electrodes is the main interference source in ignition system. Then, the paths of conducted interference and the reason of radiated emission are discussed in detail. Finally, two kinds of power supply cord EMI filters are designed and implemented on the basis of analysis results. Experimental results indicate that the filter can reduce the noise of power-wire in broadband range. Simultaneously, the radiation emission performance from power wire is also reduced observably. The proposed mixing filter has been demonstrated in this paper to perform satisfactorily with excellent effectiveness in suppression of conducted EMI from 10 MHz to 110 MHz, and the object car can also pass the EMC standard tests.     

Fast modeling of the motor broadband equivalent circuit model

This paper proposes a modeling method of the motor broadband equivalent circuit model. The impedance amplitude-frequency characteristics of the motor terminal through measuring are obtained. The impedance amplitude-frequency characteristics are devided into several regions based on its first resonant point information,and the motor broadband circuit topology is developed with each region equaling a resonance unit. The RLC parameters of resonance unit are extracted through frequency and corresponding impedance of the any non-resonant and resonant point in each unit. This proposed method is used to build DC and AC motor broadband equivalent circuit model. Their impedance amplitude-frequency characteristics are simulated by the Saber software. The measurement results and the simulation result of references are compared. The DC motor simulation results coincide with the measurement results in 0.1~50 MHz,and the AC motor is in 0.1~40 MHz. The results show that the motor broadband circuit model is reliable and feasible.