微胶囊技术及其在食品工业中的应用研究

利用微胶囊技术,可以解决传统工艺所不能解决的众多问题,生产出多种高新产品,且具有良好的功能性质和贮存稳定性。介绍微胶囊技术的特点,壁材、芯材的类型,制备方法、原理,以及其在饮料工业、乳制品、糖果、食品添加剂以及焙烤食品中的应用。     

活化风蚀坑沙障固沙技术及植被恢复

风蚀坑活化形成的流动沙地直接危害草场,使土地逐渐丧失生产力。结合风蚀坑内风速变化特征,采取风蚀坑及边坡、风蚀坑积沙区沙障固沙技术,恢复风蚀坑植被。结果表明:风蚀坑及边坡设置黄柳沙障3 a,植被覆盖率达到27.0%,植物种为7种;PE纱网沙障植被覆盖率为31.0%,植物种为11种;PLA沙障植被覆盖率达到15.6%,植物种为4种;黄柳+PE复合沙障植被覆盖率达到75.3%,植物种为6种,而对照植被覆盖率为1.6%,植物种2种。在风蚀坑积沙区,设置沙障3 a后,PE纱网沙障植物种达到10种,黄柳+PE沙障为8种,PLA沙障为4种,黄柳沙障6种,而对照为1种;植被覆盖率分别达到50.3%、50.7%、22.7%、40.0%,而对照为4.0%。采取网格或带状PE沙障,带状黄柳+带状PE沙障对风蚀坑植被恢复具有良好效果。     

hrpZ_(Psta)在转基因大豆中定量表达与疫霉根腐病和灰斑病抗性相关研究

以T5转hrpZPsta基因大豆JN29-705-15和JL30-187为材料,利用实时荧光定量PCR技术(Quantitative Real Time PCR,qRT-PCR)检测了目标基因在转基因大豆不同组织中的表达量,分别利用下胚轴侵染法和叶面喷施法鉴定疫霉根腐病抗性和灰斑病抗性,并分析目的基因表达量与疫霉根腐病和灰斑病抗性的相关性。结果表明:hrpZPsta基因在大豆的叶、茎、根、籽粒中均有表达,二个株系平均相对表达量分别为8.2/6.1、0.9/0.7、6.5/4.6和0.8/0.7;T5转hrpZPsta基因大豆抗疫霉根腐病和灰斑病能力与野生型相比均有所提高,JN29-705-15对疫霉根腐病抗性从感病提高到中抗,而JL30-187从中抗提高到抗病;hrpZPsta基因在叶中的表达量也与抗灰斑病能力呈正相关,与病情级别呈极显著负相关。试验结果初步证明了外源基因hrp ZPsta在大豆植株中的表达量与受体植株对疫霉根腐病和灰斑病抗性存在一定的相关性。     

叶面喷施外源物质对黄瓜商品苗贮运质量的影响

为了探究叶面喷施外源物质对黄瓜商品苗贮运质量的提升效果,研究蔬菜育苗生产中常用的寡聚糖、壮苗1号和植物益生源对贮运0、3、6 d黄瓜穴盘苗质量的影响。结果表明:与喷施清水的对照相比,壮苗1号可以抑制幼苗徒长,增加地下部干质量,维持较高的壮苗指数、比叶重和叶绿素含量,而植物益生源和寡聚糖对幼苗各生长指标的影响较小。因此,推荐叶面喷施壮苗1号来提高黄瓜商品苗的贮运质量。     

小径木梯形板锯刨机结构设计与研究

针对目前市面上加工小径木的机器加工精度低、效率不高、出材率低等问题，分析提高小径木梯形板出材率、加工效率及加工精度的设计方法，通过新的设计方法制定了新的加工工艺。结合工艺分析设计了小径木梯形板锯刨机的总体结构，可以对小径木梯形板进行锯切、粗刨、精刨加工。在对其核心组件锯切组件与刨削组件的加工原理进行分析的基础上，设计了锯切组件结构与刨削组件结构。通过导入锯切主轴SolidWorks的三维模型，利用ANSYS对其锯切主轴进行静力学分析，得到锯切主轴的应力、应变和变形云图，其中最大应力值为7.435 MPa、最大应变值为0.042 316 mm/m、最大变形量为0.002 178 8 mm，其强度、刚度及变形量均在许用范围内，仿真验证了锯切主轴的设计的合理性。     

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Seed longevity is a very important characteristic controlling seed quality. However, the mechanism underlying this characteristic is poorly understood. In this study, 'FH7185', a storable rice variety, the germination rate of which was 66.63%, much higher than the control after artificial ageing under relative humidity 88% and 42°C for 21 days. Different methods were applied to reveal the involvement of proteins during ageing of rice seeds. In total, 35 differential proteins were identified from 2D‐PAGE, and 3,719 proteins from iTRAQ analysis. A comparison of these two methods showed that not all protein types could be detected on the 2D‐PAGE gels, and the dynamic range was somewhat limited. So the comprehensive proteome map from the iTRAQ analysis was used to identify the quantitatively regulated proteins that played roles in seed longevity, and found that the most marked change was the increased abundance of many metabolic enzymes, especially the ones involved in α‐linolenic acid metabolism in the embryos during ageing. OsLOX2 and OsLOX3 had a negative effect on seed longevity, which were lower in FH7185 than the control. The dehydrogenase (LOC_Os07g44430) which played a major catalytic role in lipid peroxidation, also changed significantly during ageing. Therefore, OsLOX2, OsLOX3 and Loc_OS 07G44430 may be involved in the regulation of seed longevity with a synergistic effect.