Genome-wide Association Study on Alive Litter Size Trait in Bama Xiang Pigs

In order to identify the molecular markers related to alive litter size of Bama Xiang pigs,the genome-wide association study (GWAS) was used to map and screen the candidate genes affecting the alive litter size trait.Ear tissue samples of 297 Bama Xiang pigs with multiple parity records were collected,and DNA was extracted and genotyped by porcine 50K SNP beadchip.After quality control and genotype imputation,the alive litter size of Bama Xiang pigs were GWAS by Tassel.The results showed that the average number born alive per litter of Bama Xiang pigs increased gradually with the increasing of parity in the range of 1-9 parities.A total of 32 816 SNPs were obtained after quality control and filtration.8 SNPs related to alive litter size of Bama Xiang pigs were screened by genome-wide association analysis,which were significant at genome or chromosome level.Based on the enrichment analysis of the coding genes in the region between 500 kb upstream and downstream of the associated significant SNP loci,and the QTL regions and gene functions related to porcine reproductive traits,4 genes (CAPZB,MSH3,CITED2 and HSD17B7) were finally identified to be candidate genes related to alive litter size of Bama Xiang pigs.     

Study on Synergistic Effect of VA5 Immunopotentiator on Pigeon NDV Inactivated Vaccine

This study was designed to evaluate the synergistic effect of VA5 immunopotentiator on pigeon ND4416 inactivated vaccine.160 healthy pigeons were randomly divided into four groups,including ND4416 strain inactivated vaccine group (NDV group),ND4416 strain inactivated vaccine and immunopotentiator (VA5) mixed group (NDV+VA5 group),La Sota inactivated vaccine group (La Sota group) and normal saline as the control group (C group),to assess their vaccine efficacy against virulent pigeon NDV by serological analysis and animal testing.Pigeons sera were collected at different time points after immunization and measured the HI antibody titer of each group.The results showed that VA5 immunopotentiator significantly improved the serum antibody level of pigeons immunized with pigeon Newcastle disease inactivated vaccine (P<0.05).In addition,comparative test of spleen lymphocyte transformation was conducted at various time points after immunization.The results indicated that VA5 effectively stimulated the lymphocyte transformation of immune pigeon.Pigeons in each groups were challenged with ND4416 strain at the 30th,90th and 180th d after immunization.The results presented that the NDV+VA5 group had 100% protection rate and higher than La Sota group.The duration of immunization test showed that the antibody titer of NDV+VA5 group reached peak 11.20log₂ at the 21st d,remained 7.50log₂ at 180th d,and the protection rate remained 100% at 180th d.It indicated that VA5 immunopotentiator sustained the immune duration of pigeon NDV vaccine up to 180 d.Moreover,the in vitro detoxification test results suggested that VA5 immunopotentiator reduced the in vitro detoxification cycle of pigeons after challenge.Overall,this study suggested VA5 immunopotentiator could significantly improve the immune efficacy of pigeon Newcastle disease inactivated vaccine,which provided a basis for further enhancing the efficacy of Newcastle disease vaccine,as well increased experimental data for the application of immunopotentiators.     

云南、福建、湖南烟区烟草花叶病主要病毒种类检测及黄瓜花叶病毒亚组鉴定

 采用抗原直接包被和双抗体夹心酶联免疫吸附测定法(ELISA)对采自云南、福建、湖南烟区烟草花叶病样品进行了病毒种类检测,利用三抗体夹心ELISA对黄瓜花叶病毒(Cucumber mosaic virus,CMV)的亚组类型进行了鉴定。在云南采集的520个花叶病样品中,烟草花叶病毒(Tobacco mosaic virus,TMV)、CMV和马铃薯Y病毒(Potato virus Y,PVY)总检出率分别为71.74%、55.01%和6.35%;在福建采集的150个花叶病样品中,TMV、CMV和PVY的总检出率分别为94%、24.66%和8.00%;在湖南采集的74个花叶病样品中,TMV、CMV和PVY的总检出率分别为58.11%、51.35%和2.70%。部分样品为2种以上病毒复合侵染。云南、福建和湖南采集的64个CMV阳性样品中,属亚组Ⅰ的样品为57个,占89.1%;属亚组Ⅱ的样品为10个,占15.6%;其中3个样品为亚组Ⅰ和亚组Ⅱ的复合侵染。     

当前广西柑橘无病苗木生产和推广工作中应当重视的几个问题

在2005年12月由广西区农业厅和桂林市人民政府联合举办的“全区成功防治柑桔黄龙病交流培训会”的推动下，全区各地都将柑桔无病苗木生产提到了政府议事日程上，一些主产区已建起了自己的无病苗圃，开始柑桔无病苗木的生产。2006年国家农业部下达“广西桂林市柑桔无病良种繁育基地”项目由广西柑桔研究所承担。     

黑皮黄瓤小西瓜新品种雪峰小玉8号的选育

雪峰小玉8号是湖南省瓜类研究所最新育成的长椭圆形、黑皮、黄瓤、耐贮运二倍体小果型西瓜新品种。植株生长势强,抗病抗逆性强,坐果性好。全生育期85d左右,果实发育期25d左右。皮厚约0.7cm,果皮硬度26kg/cm2,不裂果;中心可溶性固形物含量12%～13%;单果重2～3kg,667m2产量2000～3500kg。2007年2月通过湖南省农作物品种审定委员会审定。     

繁殖性状的杂种优势与基因效应分析

测定杜长大三元杂交体系和斯格配套系各杂交组合的繁殖性能,计算出繁殖性状的杂种优势率;选用5个与繁殖性状紧密连锁的微卫星标记对测定个体进行基因型检测,利用混合效应线性模型计算出繁殖性状各位点的基因效应。结果表明:斯格祖代猪与父母代猪产仔数、产活仔数、断奶仔数、断奶窝重的差异均达到极显著水平(P<0.01),父母代猪与祖代猪的初生窝重差异显著(P<0.05),斯格父母代猪的产仔数、产活仔数、断奶仔数和断奶窝重与长大母猪的差异均达到极显著水平(P<0.01),斯格父母代猪与长大母猪的初生窝重差异显著(P<0.05),初生重、断奶重杂优率低,其余各性状的杂种优势率处于较高水平。显性效应在初生窝重、断奶窝重的杂种优势中起着很重要作用,加性效应是初生重和断奶重杂种优势的主要遗传学基础,产仔数、产活仔数、断奶仔数的杂种优势的产生存在上位效应。     

农牧交错区耕地净第一性生产力动态变化研究——以陕西榆林市榆阳区为例

以榆林市榆阳区1984～2003年各乡镇的粮食产量统计数据为依据,选用了适合农用地的净第一性生产力估算模型,对该区耕地净第一性生产力单位面积量及总量进行分区动态测算,以揭示生态环境对耕地净第一性生产力的影响.研究表明:(1) 1984～2003年间,榆阳区耕地净第一性生产力总量在波动中上升,20 a内总物质量从21.94×104 t/a增加到57.98×104 t/a,年度递增率为5.24%.(2) 在1984～2001年间,榆阳区北部耕地净第一性生产力远高于南部,且呈较快上升趋势,而南部黄土丘陵沟壑区的耕地净第一性生产力呈较平缓的波动变化,且略有下降.(3) 在1984～2001年间,榆阳区城区、近郊、远郊的差异性在单位耕地面积净第一性生产力上体现为城区＞近郊＞远郊;在耕地NPP总量方面,城区、近郊和远郊都表现为在波动中上升,三区变化以远郊波动幅度最大.(4) 榆阳区耕地净第一性生产力总量的大幅增长有农业投入增加的原因,但主要得益于该区的生态环境的改善.     

猪繁殖与呼吸综合征病毒感染对猪肺泡巨噬细胞外源性抗原加工递呈相关分子和共刺激分子的影响

采用定量竞争PCR技术对猪繁殖与呼吸综合征病毒（PRRSV）感染后不同时相猪肺泡巨噬细胞（PAM）中外源性抗原加工递呈相关分子SLA-DR、Ii链和SLA-DM分子及共刺激分子CD40、CD80、CD86的mRNA转录动态进行了定量检测。结果表明,PRRSV感染后PAM的SLA-DR mRNA转录水平在3、7和14 d下调,低于对照组;感染后3 d,Ii链、SLA-DM和CD40的mRNA转录水平极显著下调（P〈0.01）;CD80和CD86mRNA转录水平也在感染后3 d明显下调（P〈0.05）。用流式细胞术检测PAM表面的SLA-DR抗原的结果表明,在感染后3 d短暂上升,7 d之后一直低于对照组。由此说明,PRRSV感染早期对猪肺泡巨噬细胞的外源性抗原加工和递呈功能产生显著影响,导致其外源性抗原递呈功能下降,进而影响机体的体液免疫应答。     

全球气候变化对松嫩草原土壤水分和生产力影响的研究

本文首先模拟计算松嫩草原土壤水量平衡,并结合未来气候情景估算松嫩草原土壤水分的变化。最后,在土壤水分研究的基础上,初步评估全球气候变化对松嫩草原生产力的影响。     

不同药剂防治射干锈病试验初探

为筛选防治射干锈病的适宜药剂,2016年7-8月在贵州省长顺县进行了申嗪霉素、戊唑醇、苯醚甲环唑3种药剂防治射干锈病药效筛选试验。结果表明,3种药剂处理对射干锈病均有良好防效,二次药后10d的防治效果均在60%以上,三次药后15d的防治效果均在80%以上,防治射干锈病,在优先使用生物农药申嗪霉素的基础上,做到与其他药剂轮换使用效果较好。