武汉市桔梗科一物种新记录——卵叶异檐花

报道了武汉市外来入侵植物新记录一种,为桔梗科Campanulaceae异檐花属Triodanis卵叶异檐花Triodanis perfoliata subsp.biflora(Ruiz et Pavon)Lammers。卵叶异檐花原产于美洲,在武汉地区是首次发现,凭证标本保存于长江文明馆(武汉自然博物馆)。     

Exogenous application of allelopathic water extracts helps improving tolerance against terminal heat and drought stresses in bread wheat (Triticum aestivum L. Em. Thell.)

The allelopathic water extracts (AWEs) may help improve the tolerance of crop plants against abiotic stresses owing to the presence of the secondary metabolites (i.e., allelochemicals). We conducted four independent experiments to evaluate the influence of exogenous application of AWEs (applied through seed priming or foliage spray) in improving the terminal heat and drought tolerance in bread wheat. In all the experiments, two wheat cultivars, viz. Mairaj‐2008 (drought and heat tolerant) and Faisalabad‐2008 (drought and heat sensitive), were raised in pots. Both wheat cultivars were raised under ambient conditions in the wire house till leaf boot stage (booting) by maintaining the pots at 75% water‐holding capacity (WHC). Then, managed drought and heat stresses were imposed by maintaining the pots at 35% WHC, or shifting the pots inside the glass canopies (at 75% WHC), at booting, anthesis and the grain filling stages. Drought stress reduced the grain yield of wheat by 39%–49%. Foliar application of AWEs improved the grain yield of wheat by 26%–31%, while seed priming with AWEs improved the grain yield by 18%–26%, respectively, than drought stress. Terminal heat stress reduced the grain yield of wheat by 38%. Seed priming with AWEs improved the grain yield by 21%–27%; while foliar application of AWEs improved the grain yield by 25%–29% than the heat stress treatment. In conclusion, the exogenous application of AWEs improved the stay green, accumulation of proline, soluble phenolics and glycine betaine, which helped to stabilize the biological membranes and improved the tolerance against terminal drought and heat stresses.     

Identification and quantification of anthocyanins in different coloured cultivars of ornamental kale (Brassica oleracea L. var. acephala DC)

Anthocyanins are responsible for the colour of many fruits, vegetables, flowers, and coloured-leaved trees. Ornamental kale (Brassica oleracea L. var. acephala DC) is widely cultivated for its colourful inner leaves. To investigate the relationship between the degree of colouration and anthocyanin distribution, content, and composition in ornamental kale, the authors studied the pigment characteristics of five cultivars with different coloured leaves (white, pink, red, purple, and purple-black). Microscopy observation, spectrophotometer, and high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of fresh inner leaves revealed that pink, red, and purple colourations were associated with high levels of anthocyanin, while purple-black was the result of the combination of anthocyanins and chlorophyll. In the coloured cultivars, anthocyanins were abundant mainly in the first and second cell layers below the epidermis in both the hypocotyls and inner leaves. No anthocyanin was found in the white-leaved phenotype cultivar. Anthocyanin content increased as leaf colour deepened from pink, red, to purple cultivars, which had little chlorophyll and carotenoid. The authors identified eight anthocyanins in the four coloured cultivars, including one non-acylated, four monoacylated, and three diacylated cyanidin glycosides. Cyanidin-3-(sinapoyl)(feruloyl)-diglucoside-5-glucoside was the most abundant anthocyanin in the four coloured cultivars followed by cyanidin-3-(sinapoyl)-diglucoside-5-glucoside. The analysis of anthocyanin accumulation characterisation provides important information on evaluating colouration patterns in coloured plants, and will be helpful for breeding desired leaf colours in ornamental kale.     

New Polyketides from the Antarctic Fungus Pseudogymnoascus sp. HSX2#-11

The species Pseudogymnoascus is known as a psychrophilic pathogenic fungus with a ubiquitous distribution in Antarctica. Meanwhile, the study of its secondary metabolites is infrequent. Systematic research of the metabolites of the fungus Pseudogymnoascus sp. HSX2#-11, guided by the method of molecular networking, led to the isolation of one novel polyketide, pseudophenone A (1), along with six known analogs (2–7). The structure of the new compound was elucidated by extensive spectroscopic investigation and single-crystal X-ray diffraction. Pseudophenone A (1) is a dimer of diphenyl ketone and diphenyl ether, and there is only one analog of 1 to the best of our knowledge. Compounds 1 and 2 exhibited antibacterial activities against a panel of strains. This is the first time to use molecular networking to study the metabolic profiles of Antarctica fungi.     

法氏囊活性肽在大肠杆菌中的克隆与表达

法氏囊活性肽（Bursin,BS)作为一种免疫增强剂，临床应用广泛，为了大量制备BS，按大肠杆菌惯用密码子合成BS基因。并用色氨酸将5个BS基因串联，克隆于质粒pU57的SmaI位点，经测序证明序列正确后，以PCR扩增，克隆于表达载体pBV220的EcoRI,HinDⅢ位点，温敏诱导表达，其表达水平占全菌总蛋白的13.4%。     

青霉素V钾滴丸的研制

为减少青霉素V钾的水解并提高其疗效，用聚乙二醇400和聚乙二醇6000为基质制备青霉素V钾滴丸。体外抑菌试验表明，青霉素v钾滴丸抑菌作用略强于人药片剂。     

福建黄兔繁殖性状相关研究

本文对３１０只母兔的繁殖性状进行了测定分析，结果表明；妊娠期与产仔数、产活仔数及活仔窝重呈负相关，相关系数分别为－０．４２６＊、－０．３５０＊和－０．５１５头４胎内母兔，胎次对产仔性能无影响，对母兔泌乳性能有一定影响。同样条件下胎次增加。泌乳性能提高，第３胎较第２胎有显著提高。     

樟子松枯梢病的防治研究

樟子松移植苗枯梢病Sphaeropsis sapinea可以通过药剂有效地进行防治。移植前以50％多菌灵wp500倍液浸苗；在人工林中，内吸性杀菌剂－甲基托布津、多菌灵为首选药剂；在树木新梢生长停止期和展叶中期（辽西北为6月初和6月中旬），70％甲基托布津wp 1000倍液或50％多菌灵wp 500倍液防治2次。保护性杀菌剂－百菌清效果不佳。适时的卫生伐对该病有明显的抑制作用。     

黄皮的生物学特性研究

通过本试验观察表明:黄皮[Clausena Lansium (Lour.)Skeels]的开花与温度有密切关系,温度高,开花过程短;温度低,开花过程长。花穗中的花轴增长速度也随温度升高而加快,花轴增长快,则花轴长,花穗大;反之,花轴增长速度慢,花轴短,花穗小。黄皮开花的顺序是从花穗基部第1—4个分枝开始,每个分枝则先从基部1—8个二次分枝首先开放。31℃是黄皮花粉萌发最适宜温度。在培养基中加入15—20％蔗糖或2.4—D,三十烷醇等生长调节物质,明显可提高黄皮花粉的萌发力。本试验对黄皮的物候期及植物学特性同时进行了研究。     

树木挥发物对植食性昆虫的诱导抗性

树木挥发物质对植食性昆虫取食，产卵所产生的诱导抗性是树木与昆虫的长期协同进化过程中的必然结果，树木与植食性昆虫之间发展了一套互相调节，自身开拓资源，产生生存对策的复杂相互关系，本文着重阐明了树木与植食性昆虫的互相策略，诱导抗性的生物化学基础以及诱导抗性在调节植食性昆虫种群中的重要性。