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1.
2003~2005年对福建中、东部沿海的主要贝类养殖区养殖贝类进行质量抽检,品种包括菲律宾蛤仔、文蛤、牡蛎、贻贝及缢蛏,检测项目为汞、铅、砷、镉、石油烃等,并对检测结果进行分析讨论,结果表明大部分贝类产品卫生质量状况良好。 相似文献
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对大连地区5种常见贝类及其生长环境海水养殖用水重金属铅(Pb)、镉(Cd)的含量状况进行了研究调查,评价该地区贝类重金属污染情况及人类食用潜在健康风险,并为合理控制该地区重金属污染提供科学依据。结果显示重金属含量均符合相应标准。重金属污染指数评价结果表明,虾夷扇贝、栉孔扇贝、牡蛎中含量属于轻污染水平,菲律宾蛤仔、贻贝含量尚处于正常浓度值范围。各贝类对Pb、Cd的富集能力大小顺序为:栉孔扇贝>虾夷扇贝>牡蛎>贻贝>菲律宾蛤仔。危害商数<1,不具有潜在的健康风险,可以放心食用。 相似文献
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ABSTRACT: Paralytic shellfish poisoning (PSP) toxins produced by Alexandrium isolates from Korea were analyzed by high-pressure liquid chromography. Species designation of the regional isolates was determined by morphological criteria and ribotyping inferred from sequences of the 28S rDNA D1-D2 region. Toxin analysis performed at the exponential growth phase, revealed that the two strains of A. fraterculus were non-toxic, while the strains of A. tamarense and A. catenella were toxic. Toxic isolates DPC7 and DPC8 of A. catenella produced GTX1, 2, 3, 4, 5, dcGTX2, 3, C1, 2, neoSTX and STX with trace or non-detectable levels of C3 and C4, while isolates UL7, KDW981, SJW97043, SJW97046, KJC97111 and KJC97112 of A. tamarense produced GTX1, 2, 3, 4, dcGTX3, C1, 2, neoSTX with trace or non-detectable levels of C3, 4, dcSTX and STX, and no GTX5 and dcGTX2. The major toxins produced by A. catenella were C1 +2, and those of A. tamarense were C1 +2 and GTX4 in most of the isolates. A. tamarense strains other than SJW97046 produced a relatively high proportion of carbamate toxins, reflecting the high toxicity scores of shellfish intoxication in sampled coastal areas. Two representative toxic isolates, A. tamarense SJW97043 and A. catenella DPC7, were cultured for 30 days in batch mode and subjected to toxin analysis at 5-day intervals. Comparison of toxin productivity in terms of total toxin content, toxin components, and their variations with culture age revealed marked differences between the two strains. 相似文献
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本文介绍用紫外线法消毒的海水对文蛤、花蛤、缢蛏进行净化的中试生产工艺技术及其净化效果。通过对该三种净化贝净化前后的各种安全指标以及其净化过程中大肠菌群数和砂份含量随时间的变化进行检测,认为原料符合SC/T 3013-2002规范的文蛤、缢蛏、花蛤经24小时的净化其大肠菌群都能达到DB35/575-2004净化海水贝类标准的要求(≤300MPN/100g贝肉),同时大肠群菌数较低的贝类可以缩短净化时间;根据其含砂量的不同净化时间可控制在8~16小时;要通过净化来大量降低贝类的重金属、农药及贝毒的含量似乎是不可能的;根据贝类品种的不同,其净化过程的死亡率在1.1~3.5%,产品得率90~98%;其净化产品在8℃~10℃贮存与流通可贮藏7天。 相似文献
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Ryoma?KamikawaEmail author Shoko?Hosoi-Tanabe Satoshi?Nagai Shigeru?Itakura Yoshihiko?Sako 《Fisheries Science》2005,71(5):987-991
ABSTRACT: The cysts of toxic dinoflagellate Alexandrium tamarense are the seed population for the bloom responsible for paralytic shellfish poisoning (PSP). However, it is impossible to identify the Alexandrium spp. cyst on the basis of morphological features. In this study, we prepared A. tamarense cysts by sexual conjugation in laboratory conditions and developed an efficient DNA extraction method for polymerase chain reaction (PCR) assay. Using the A. tamarense cysts, we established the identification and quantification method showing the species specificity and the high sensistivity for A. tamarense cysts using real-time PCR. This assay was also able to detect and quantify the A. tamarense cysts accurately when mixed with excess cysts of A. catenella (Whedon and Kofoid) Balech prepared by conjugation experiment. 相似文献
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ABSTRACT: The toxic dinoflagellates Alexandrium tamarense (Lebour) Balech and A. catenella (Whedon and Kofoid) Balech produce potent neurotoxins, such as saxitoxin and gonyautoxin and have been mainly responsible for paralytic shellfish poisoning (PSP) in Japan. To prevent a negative effect on the fishery industry, it is necessary to identify these toxic species precisely and rapidly before and during the bloom. In this paper, a rapid and simple protocol of a fluorescence in situ hybridization (FISH) method using ribosomal RNA (rRNA)-targeted probes has been established for identifying the cultured strains and natural cells of A. tamarense and A. catenella . Using the FISH method established in this study, it was possible to identify these toxic species species-specifically and rapidly, within 30 min. The procedure of detection constituted three steps: (i) fixation/dehydration; (ii) hybridization; and (iii) washing; this made the identification simple. Moreover, this method did not require either special techniques or equipment, and the cost for detection was low. The specificity, rapidity, and simplicity of the developed method suggest that it might be useful for routine monitoring of these toxic microalgae. 相似文献