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通过建立醋酸致小鼠毛细血管通透性增强模型,二甲苯致小鼠耳廓肿胀模型和角叉菜胶致大鼠足趾肿胀模型,研究蒲公英甾醇粗提物对炎症的抑制作用.设立模型组,蒲公英甾醇粗提物组和阳性组,蒲公英甾醇粗提物组分别给予蒲公英甾醇750、375、187.5 mg/kg,连续灌胃5d,观察蒲公英甾醇对小鼠毛细血管通透性、耳廓肿胀和大鼠足趾肿胀的抑制作用.结果显示,蒲公英甾醇对醋酸致小鼠毛细血管通透性增强,二甲苯致小鼠耳廓肿胀和角叉菜胶致大鼠足趾肿胀均有显著抑制作用.表明蒲公英甾醇具有明显的体内抗炎作用. 相似文献
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蒲公英提取物对小鼠腹腔巨噬细胞释放一氧化氮的影响 总被引:10,自引:0,他引:10
用MTT法测定蒲公英提取物对脂多糖(LPS)激活小鼠腹腔巨噬细胞的毒性作用,以确定无细胞毒性剂量范围,并在该剂量范围内采用Griess试剂比色法测定蒲公英提取物对LPS激活小鼠腹腔巨噬细胞分泌一氧化氮(NO)的影响,为蒲公英提取物药理作用的进一步研究提供依据.结果表明,蒲公英提取物≤1mg/mL剂量范围内对巨噬细胞无细胞毒性作用.其在0.25~1 mg/mL浓度范围内对LPS刺激的小鼠腹腔巨噬细胞分泌NO具有明显的抑制作用,且呈剂量和时间依赖关系. 相似文献
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为了研究蒲公英对乌骨鸡免疫功能的影响,试验采用单因子方差分析进行试验设计、分组,以拌料法和饮水法给乌骨鸡饲喂蒲公英,通过测定不同日龄乌骨鸡体重、抗NDV血清抗体效价、免疫器官(胸腺、脾脏、法氏囊)重量和指数,研究其对乌骨鸡增重和免疫功能的影响。结果表明:与对照组相比,2个试验组增重差异显著;蒲公英能促进乌骨鸡抗NDV抗体的产生;对乌骨鸡脾脏发育有一定的抑制作用,对法氏囊的影响因给药方式不同而有一定差异,对胸腺发育有一定的促进作用。说明蒲公英对乌骨鸡免疫功能具有一定影响。 相似文献
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为了研究蒲公英对肉鸡新城疫免疫效果的影响,试验采用蒲公英干燥粉按照不同比例拌料法饲喂,通过测定不同日龄抗NDV血清抗体效价及免疫器官指数测定,研究其对肉鸡新城疫免疫效果。结果表明:蒲公英2.0%饲料添加量可增强肉鸡新城疫免疫效果。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献