共查询到18条相似文献,搜索用时 187 毫秒
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本研究出桑树染色体制片的直接酸解击壁法:直接取桑嫩芽幼叶投入“酸醇去壁固定液”(浓盐酸:45%醋酸,纯乙醇=1:0.5:0.5容积比)中3~5min,然后将材料水洗几次浸入水中后低渗10min,再经染色即制备成染色体标本。验证了在保证染色体制片观察效果前提下,样本材料采集后可在室温和冰箱(7~12℃)保存数小时至36小时。并与其它制片方法从制片效果,工作效率、成本等方面进行比较。从而证明本方法具有操作简单、省时省力、成本低、效率高、有较大的实际应用价值。 相似文献
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紫茉莉染色体数目及核型分析 总被引:3,自引:1,他引:2
紫茉莉Mirabilis jalapa为多年生草本花卉,繁殖能力强,被确定为外来入侵植物。用低温处理法处理紫茉莉根尖12h,经卡诺氏液固定、酸解去壁、改良品红染色等步骤,对紫茉莉进行了染色体数目的确定,对其核型进行了分析。试验结果表明,紫茉莉体细胞染色体数目为2n=56,核型公式为K=2n=2x=28m+22sm+4st+2m(sat),核型分类属于"2B"型。染色体长度变异范围为2.33~0.78μm,属小型染色体,不对称系数63.8%,属于较不对称类型。这为紫茉莉的防控提供了细胞学依据。 相似文献
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本文以生成的还原糖含量为指标,探讨了机械粉碎法、稀酸法、稀碱法、微波法、微波结合酸减法、有机溶剂法和温度变化法预处理对桑树枝条酶解的效果。结果表明:7种预处理方法对桑树枝条都有不同程度的破坏,能够有效提高桑枝条的酶解产糖量。机械粉碎度越大酶解效果越好;1%硫酸和10%NaOH为酸碱的最佳处理浓度;180 w低功率微波处理1 min效果显著高于高功率长时间效果;不同强度微波结合1%稀硫酸的效果比单独的微波效果低,但结合10%NaOH比单独的微波效果高;有机溶剂丙酮效果明显高于甲醇和乙醇;在常压下,低于70℃时随着温度的升高,酶解效果增强,高于70℃后酶解效果趋于平缓,加压后酶解效果大大增强。不同的预处理方法,效果各有大小,各有利弊。 相似文献
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近年来,在WHO倡导的血吸虫病二元化防治策略的指导下,对血吸虫中间宿主-钉螺的研究日渐深入。取材问题、秋水仙素浓度、低渗处理时间和染色体的制备方法是成功制备钉螺染色体标本的关键。钉螺染色体核型主要包括染色体的数目和染色体形态。地域差异或者不同地理种群是造成染色体核型差异的主要原因,染色体显带技术的发展,为钉螺染色体核型研究提供了技术基础。论文对钉螺染色体核型研究进行综述。 相似文献
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研究检测酸乳塑杯封口盖膜揭开力的测试方法,利用专门的力值检测设备完全模拟人机操作对酸乳塑杯盖膜揭开力进行量化,主要表征为初始揭开力和持续揭开力.并以聚丙烯(polypropylene,PP)和聚苯乙烯(polystyrene,PS)2种塑杯,铝箔复合膜、镀铝复合膜、压花铝片3种封口盖膜组合的市售酸乳包装为实验对象,通过该测试方法进行塑杯包装封口盖膜揭开力测试,最终得到不同包装形式相应的盖膜揭开力力值范围.结果表明:塑杯封口盖膜的初始揭开力明显大于持续揭开力,PS、PP塑杯封口盖膜初始揭开力值为10~25 N;以压花铝片为封口材料时,塑杯封口盖膜的持续揭开力大于10N,以铝塑复合膜为封口材料时,塑杯封口膜的持续揭开力仅为4~8N;同种封口盖膜,PS塑杯的盖膜揭开力大于PP塑杯.而同种塑杯,镀铝封口膜的初始揭开力高于压花铝片和铝箔封口膜,压花铝片的持续揭开力高于镀铝封口膜和铝箔封口膜,差距悬殊.且以压花铝片为封杯材料时,盖膜初始揭开力与持续揭开力的差值会明显小,封杯强度相对稳定. 相似文献
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本文报道了蓝舌病相关病毒Eubanangee RNA 2与RNA 3 的cDNA克隆及序列分析。采用随机引物将纯化的病毒的dsRNA的第二,三片段逆转录成cDNA,用Sau3AI消化,克隆于BamHI酶切的M_(13)载体中,然后用Sanger双脱氧链终止法进行核酸的序列分析。结果表明:RNA3与BTV(蓝舌病)WJRNA3的同源性达70%以上,而RNA 2与BTV的RNA 2同源性较小。 相似文献
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氢化物发生-原子荧光光谱法同时测定饲料中的砷、汞、硒和镉 总被引:1,自引:0,他引:1
研究采用消化罐消解方法,应用氢化物发生-原子荧光光谱法同时进行饲料中砷、汞、硒、镉的测定,并对各种试验条件及影响因素进行了探讨。结果表明:采用HNO3-HClO4体系在消化罐内进行湿法消解,样品消解完全且所用时间短;使用HCl作为反应介质效果良好;在一定浓度范围内4种元素的浓度与荧光响应值均有良好的线性关系;4种元素的加标回收率均在90%以上;精密度(RSD,n=11)不大于1.7%。建立的方法简便、稳定可靠,检测成本低,结果满意,可用于饲料中砷、汞、硒和铅的同步检测。 相似文献
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酶消化法分离培养肉鸡肺动脉平滑肌细胞 总被引:1,自引:0,他引:1
试验旨在探讨胶原酶消化法分离并培养肉鸡肺动脉平滑肌细胞(pul monary artery smooth muscle cell,PASMC)的方法。将1~7日龄雏鸡的肺动脉中膜剪成小于1 mm2的组织块,0.1%Ⅱ型胶原酶消化分离PASMC并进行原代培养和传代培养。倒置显微镜下观察培养细胞的形态,对培养细胞进行HE染色和光学显微镜观察,并采用抗α-肌动蛋白单克隆抗体(α-Smooth muscle actin)免疫组化染色法检测细胞胞浆内α-肌动蛋白的表达,用以鉴定所培养的细胞。结果表明,倒置显微镜下细胞为长梭形,呈典型的"峰—谷"状,未见明显异型细胞。α-actin胞浆染色阳性细胞数占总细胞数的97%以上。因此,胶原酶消化法可用于分离培养肉鸡PASMC,且有方法简单,成活细胞数多、传代周期短的特点,可用于肉鸡心血管疾病如肺动脉高压和肺血管重构的研究工作。 相似文献
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J Brown J L Blue R E Wooley D W Dreesen L E Carmichael 《Journal of the American Veterinary Medical Association》1976,169(11):1214-1216
In a serologic survey of stray and pet dog populations of Georgia, serums were screened for Brucella canis antibodies, using the slide agglutination test. If results were positive, B canis antibody titers were determined, using the standard tube agglutination test. The stray dogs had significantly (P less than 0.01) higher titers than did the pet dogs. The reactor rate was 58% higher for the slide agglutination test than for the tube agglutination test. The manufacturer's evaluation of the slide agglutination test was based on a comparison of the serologic results of that test with those of the tube agglutination test, using a comparative method that permitted the results to be interpreted as 99% agreement between the 2 tests. Reevaluation of the manufacturer's data by a different method indicated that the slide agglutination test is very accurate when the results are negative (99.7% specific) but less so when the results are positive (62.5% sensitive). 相似文献
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Nine ruminally and duodenally cannulated steers (average BW 355 kg) were used to evaluate effects of prebloom alfalfa greenchop substitution at 20% of DMI on utilization of late-May (high quality; HQ; Period 1) and mid-August (low quality; LQ; Period 2) tall fescue greenchop. Alfalfa inclusion did not influence (P greater than .10) diet ad libitum DMI during Period 1 but it decreased (P less than .10) DMI during Period 2. Ruminal and total tract DM, cell wall, and GE digestibility of HQ were unaffected (P greater than .10) by alfalfa inclusion; however, digestibility of these constituents in LQ was increased (P less than .03) by alfalfa substitution. Alfalfa substitution did not influence (P greater than .10) dietary cell wall monosaccharide disappearance. Ruminal CP digestibility was greater (P less than .10) when steers received alfalfa, but microbial efficiency (grams of bacterial N/kilogram of OM truly digested in the rumen) was not enhanced (P greater than .10) by alfalfa inclusion in either HQ or LQ diets. There was a trend (P = .15) for greater microbial efficiency with alfalfa substitution to LQ. Ruminal particulate passage rate did not differ (P greater than .10) between treatments for either stage of maturity. Fluid passage rate was faster (P less than .10) in steers that received only LQ (7.1%/h) than in those fed LQ substituted with 20% alfalfa (5.0 %/h). Our data suggest that alfalfa inclusion in a low-quality fescue diet enhanced cell wall and GE digestibility. 相似文献
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聚乙二醇引发对几种牧草种子发芽率和活力的影响 总被引:26,自引:4,他引:22
应用聚乙二醇(PEG)引发法探讨了不同引发渗透势、时间处理对无芒雀麦、苏丹草、沙打旺、小冠花、紫花芷蓿等牧草种子萌发和活力和有性在适宜处理下供试种子发芽速度、发芽率及活力指数分别显著高于对照;PEG的引发作用还表现为提高活力效应大于提高发芽率效应,学表明,低渗透势(-0.1、-1.2、-1.5MPa)引发处理对提高发芽率无作用;但处子早期萌发的效应对于沙打旺和小冠花种子的2仍很强烈,对苏丹草种子则 相似文献
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A simple technique is described for the rapid diagnosis of rabies in formalin-preserved brain. Brain tissue was emulsified, washed with phosphate-buffered saline and digested with trypsin. The digested material was stained according to conventional immunofluorescent procedures. Digestion with trypsin markedly enhanced the staining of rabies inclusions and eliminated non-specific staining of formalin-preserved brain. The method seems to be more accurate than histological examination. 相似文献