安徽省猪呼吸道疾病五种病原的分离与鉴定

猪呼吸系统疾病目前是困扰我国养猪业主要疾病之一。为了弄清安徽省猪呼吸道疾病主要致病微生物,本研究利用细菌学鉴定技术、RT-PCR技术对2010年-2011年安徽省皖南、皖中、皖北地区采集的180份呼吸障碍性病(死)猪病料进行了猪繁殖与呼吸综合征病毒、圆环病毒2型、链球菌、巴氏杆菌、副猪嗜血杆菌等5种病原进行了分离鉴定。结果显示,猪繁殖与呼吸综合征病毒感染率高达68%,圆环病毒2型感染率高达83.3%;猪繁殖与呼吸综合征病毒与圆环病毒2型混合感染率达27.8%,猪链球菌感染率为22.8%。结果提示,猪繁殖与呼吸综合征病毒缺失株、圆环病毒2型、链球菌的普遍感染是近年安徽省猪呼吸道疾病的重要致病病原,研究结果为安徽省今后猪群呼吸道疫病防控策略的制定提供了参考依据。     

温和型猪瘟混合感染的综合预防和治疗

温和型猪瘟往往混合感染猪圆环病毒、猪繁殖与呼吸综合征病毒，造成免疫抑制，从而继发感染各种致病菌。采取抗病毒、抗菌和激活免疫系统、肠道调理等多种手段，可以极大地降低死淘率。     

猪高热病的诊断与防治

从2006的6月底开始,我市的许多猪场,生长育成猪和部分母猪、保育猪暴发猪无名高热病,大部分发病猪场患猪发病率在50%以上,死亡率高达50%～90%,病原主要为多种病毒和细菌、寄生虫的混合感染和继发感染,包括猪瘟病毒(HCV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪流感病毒(SIV)、伪狂犬病病毒(ADV)、猪圆环病毒2型(PCV—II)和猪胸膜肺炎放线杆菌(APP)、猪链球菌属2型(SS—II)、副猪嗜血杆菌病(HP)、多杀性巴氏杆菌(PM)、猪支原体肺炎(MH)、弓形体、附红细胞体、猪霍乱沙门氏菌(SC)等细菌的混合感染。     

副猪嗜血杆菌病及其防治措施

近年来,随着免疫抑制性疾病蓝耳病、圆环病毒病、支原体病等对猪群不同程度的侵袭,使猪群的免疫力、抵抗力严重下降,疾病的混合感染现象十分普遍,副猪嗜血杆菌病常作为继发、并发感染出现在一些猪场的保育舍和生长舍,使猪群的死淘率大大增加,给猪场造成较大损失。     

冬春季节保育猪群常见疾病及综合解决方案

<正>冬春季节保育猪群的各种健康问题普遍增多,严重影响猪场的效益。各种健康问题的形成原因十分复杂,主要包括断奶应激、各种母源抗体消减、生产管理水平较差、日粮问题、天气寒冷和常见病毒(猪繁殖与呼吸综合征病毒、猪瘟病毒和2型圆环病毒等)的持续感染等。以上各种因素相互作用造成保育猪群抵抗疾病的能力下降,进而保育猪群原发或继发感染链球菌、猪副嗜血杆菌、猪鼻支原体和猪肺炎支原体等细菌性病原。     

猪繁殖与呼吸综合征病毒、圆环病毒2型和猪瘟病毒混合感染的诊断与防制分析

2011年12月,上海某大型猪场暴发以母猪流产、产死胎和弱仔,哺乳仔猪体温升高、皮肤发绀、呼吸衰竭、顽固性腹泻,保育猪发烧、扎堆、皮肤红、昏睡、腹股沟淋巴结肿大为主要症状的疾病,死淘率高。剖杀3头发病哺乳仔猪、3头死亡保育猪,取淋巴结、脾、肺脏病变组织,用PCR、RT-PCR方法分别检测猪繁殖与呼吸综合征病毒、圆环病毒2型、猪瘟野毒为阳性,结合本病的临床症状和病理剖检,确诊为猪繁殖与呼吸综合征病毒、圆环病毒2型、猪瘟病毒混合感染。     

副猪嗜血杆菌病的特点、病因及防治

猪副嗜血杆菌病是由猪副嗜血杆菌引起，这种细菌在环境中普遍存在。近年来，随着免疫抑制性疾病——蓝耳病、圆环病毒等对猪群不同程度的侵袭，再加上大部分猪场的另1个免疫抑制性病菌一支原体的继发，使猪群的免疫力、抵抗力严重下降，疾病的混合感染现象十分普遍，在技术服务中我们发现副猪嗜血杆菌病常作为继发、并发感染出现在一些猪场的保育舍和生长舍，使猪群的死淘率大大增加，给猪场造成较大甚至是巨大损失。下面谈谈本人对此病的认识和控制方法，供各位同仁参考。     

圆环疫苗“圆毕净”在猪场的应用

正1猪圆环病毒病(PCV2)感染和免疫特点锗圆环病毒2型(PCV2)可引起断奶仔猪多系统衰竭综合征、皮炎肾病综合征、增生性坏死性肺炎、猪繁殖与呼吸综合征、仔猪传染性先天性震颤等多种疾病,临床上以PMWS最为常见。PCV2感染可导致猪群产生免疫抑制,研究发现PCV2不仅可以引起猪的原发感染,更严重的是对畜体免疫功能造成损害,造成其他细菌或病毒(猪繁殖与呼吸综合征病毒、猪链球菌、猪细小病毒、伪狂犬病病毒等)的并发或继发感染,     

湖南某猪场保育猪高死亡情况分析

为了查明湖南省某猪场保育猪厌食、发烧、咳嗽、皮肤发绀致保育猪死亡的原因,采用临床症状观察、现场剖检及实验室PCR检测等方法,结果表明,送检病死保育猪发病是由猪繁殖与呼吸综合征类NADC-30毒株和HP-PRRSV毒株及猪圆环病毒2型混合感染,继发细菌性疾病,应采取相应的治疗及预防措施,降低该病造成的损失。     

猪副嗜血杆菌病的诊断与防治

近年来，随着免疫抑制性疾病——蓝耳病、圆环病毒病等对猪群不同程度的侵袭，再加上大部分猪场的另一免疫抑制性——支原体的混合感染，使得猪群的免疫力、抵抗力严重下降，疾病的混合感染现象十分普遍。在技术服务中我们发现猪副嗜血杆菌病常作为继发、并发感染出现在一些猪场的保育舍和生长舍，使猪群的死淘率大大增加，给猪场造成较大甚至是巨大损失。现将该病的诊断与防治措施介绍如下。     

Heartworm in dogs in Canada in 1983

In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.

     

Heartworm in dogs in Canada in 1984

In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

     

Heartworm in dogs in Canada in 1985

In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1988

In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).     

Heartworm in dogs in Canada in 1989

In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.

     

Heartworm in dogs in Canada in 1987

In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%).     

Heartworm in dogs in Canada in 1986

In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).

Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.