九孔鲍育苗池附着基上底栖微藻群落及其多样性

2006年9月14至10月26日对福建省漳浦县下蔡九孔鲍(Haliotis diversicolor supertexta)育苗池进行定点采样。检测结果共鉴定底栖微藻24属62种,其中硅藻21属59种,绿藻、蓝藻和褐藻各1种。附着基上底栖微藻的优势种有12种,全部为硅藻,如盾卵形藻微小变种(Cocconeis scutellum var.parva)、小形舟形藻(Navicula parva)、咖啡双眉藻(Amphora coffeaeformis)、琴式菱形藻(Nitzschia panduriformis)等。优势种的优势度突出。常见种有25种,少见种有27种。优势种和常见种多是能够适应底栖环境的种类。附着基上底栖微藻种类及多样性指数育苗早期波动大,后期逐渐稳定,底栖微藻多样性指数平均为0.980～1.959,结果表明,底栖微藻群落结构的演变与鲍幼体摄食活动有很大的相关性。     

不同单胞藻饵料培养九孔鲍早期稚贝的研究

以单种培养的6种底栖硅藻和具有底栖习性的亚心形扁藻(Platymoras sp.)为饵料,培养九孔鲍(Haliotis diversi-color supertexta)受精后12 d的早期稚贝并观察其生长和存活,培养期为28 d,目的是筛选能维持早期稚贝高存活率和生长率的藻种。实验结果表明,培养稚贝成活率最高的3个藻种由大到小依次是爪哇曲壳藻亚缢变种(Achnanthes javanica var.)、流水双眉藻(Amphora fluninensis)和亚历山大菱形藻(Nitzschia alexandrina),使稚贝生长最快的3个藻种由大到小依次是流水双眉藻、爪哇曲壳藻亚缢变种和亚历山大菱形藻。研究结果揭示了适宜的底栖硅藻种类对鲍早期稚贝阶段生长和存活的重要性。个体小或者胞外产物量大的藻类很可能对九孔鲍早期稚贝的培养是有利的。     

底栖硅藻种类组成对皱纹盘鲍匍匐幼体和稚鲍生长、存活的影响

为验证由生产培养获得的不同质量的底栖硅藻培养皱纹盘鲍不同生长阶段幼体的效果,采用经不同培养措施获得的两类底栖硅藻群落(饵料A和饵料B)培养皱纹盘鲍前期匍訇幼体和稚鲍.试验结果表明,饵料A培养前期匍匐幼体的存活率[(43.47±13.53)%]显著高于饵料B[(6.94±5.17)%],但生长指标差异不显著.两类饵料培养的稚鲍存活率差异不显著,但饵料B的生长速度和特定生长率[(0.151±0.025)mm/d和(4.706±0.492)%/d)]显著高于饵料A[(0.103±0.022)mm/d和(3.625±0.374)%/d)].说明皱纹盘鲍幼体在不同生长阶段适应底栖硅藻的种类不同.匍匐幼体期对底栖硅藻质量要求较高,稚鲍期由于食量增加和食性转化,对饵料数量的要求表现得更为明显.     

底栖硅藻大面积培养技术

底栖硅藻是从鲍上足分化匍匐幼体附着变态到长至壳长达3-5mm的稚鲍所必需的饵料。底栖硅藻质量的优劣，直接影响到鲍苗培育的成活率。因此培养足量的优质底栖硅藻是鲍人工育苗生产中最为重要的环节。本文重点围绕高密度、优质底栖硅藻规模化培养技术进行探讨，总结了一套底栖硅藻大面积培养技术，     

浅谈单细胞藻类的培养及在海水育苗中的不同效果

1种类搭配在育苗生产中,幼体成长的好坏与饵料的营养直接相关。而多种饵料混合投喂是解决营养全面的最佳途径。如鲍的人工育苗中,在提供适量的底栖硅藻的同时,投喂含大量蛋白质的人工合成饵料以促进生长,而人工合成饵料中的相当一部分就是单胞藻,如螺旋藻、海泥中的底栖硅藻等；海参育苗已见的最佳投饵方法就是角毛藻、盐藻混和投喂,尽管使用其一甚至小新月菱形藻也能出苗,但受营养及培养条件(主要是施肥量)的影响,     

2种底栖硅藻饲养杂色鲍幼体和稚贝的饵料效果

用羽纹藻（Pinnularia sp．）、简单双眉藻（Amprhoraexigua）、羽纹藻和简单双眉藻混合（混合组）作为饵料，以自然海区采集的硅藻作对照，研究其对杂色鲍（Haliotisdiversicolor）幼体和稚贝成活和生长的影响，确定人工分离的2种硅藻的饵料效果。结果表明，2种底栖硅藻在杂色鲍育苗中具有一定的饵料价值。饵料种类对不同规格杂色鲍的成活率和生长影响显著，并且2个观测指标的结果相同。幼体羽纹藻组、简单双眉藻、混合组3组间的成活率、生长无显著差异，但都明显比对照组低；稚贝简单双眉藻、混合组和对照组无显著差异，羽纹藻组显著比对照组低。     

添加植物生长刺激素α-奈乙酸钠培养底栖硅藻的效果

海产底栖硅藻是皱纹盘鲍幼体进入匍匐期后的主要饵料。其培养的好坏和增殖的快慢，直接影响前期稚鲍的生长和发育。     

硅藻对鲍幼体着底和生长的影响

硅藻对鲍幼体着底和生长的影响日本水产大学大贝政治、广岛县水试若野真、兵库县水试长井敏研究表明，皱纹盘鲍幼体从浮游转着底时，因附着板上硅藻优势种不同，而出现着底数差别很大，且其后生长也有很大差异。附着硅藻为单一培养的卵形藻、聚生舟形藻、新月菱形藻。对照...     

稚鲍饵料的初步试验

在鲍鱼人工育苗过程中，解决稚鲍的适宜饵料及满足供应的问题是充分发挥幼体的生长发育潜力，提高成活率的关键。如何解决稚鲍饵料问题，国内外曾有一些报道。如福建省东山鲍珠站的措施是：5mm以内的稚鲍，主要靠附着板上事先培养的底栖硅藻，其后以更换附着片进行饵料的补充。5mm以上幼鲍主要投喂海藻及人工配合饵料片。日本在5mm以下幼鲍期，主要也是靠底栖硅藻类及海带，     

流溪河底栖硅藻群落结构的时空异质性及其影响因素

底栖硅藻能够较好地响应河流水环境变化,其群落物种组成与分布已被广泛应用于河流水质与生态状况的指示与评估。为了解流溪河底栖硅藻群落结构的时空异质性及其受河流水文与水质的影响,从上游到下游共设置了 20个样点,于2018年3月、6月、9月和12月进行季节采样和观测,采集底栖硅藻,同步测定了水体理化因子;在分析群落组成的基础上,进一步探究河流环境变量和空间变量对流溪河底栖硅藻群落的影响。结果表明,4次调查共检 出底栖硅藻276种,隶属于2纲、6目、9科、55属,底栖硅藻多样性具有明显的时空差异。在季节上,丰水期的物种丰 富度和真辛普森多样性指数高于枯水期。枯水期曲壳藻科的相对丰度超过50%,高于丰水期;而舟形藻科在丰水期 时相对丰度比枯水期高。在空间上,三级河流物种多样性最高,一级河流物种多样最低;一级河流和二级河流的优 势种主要以曲丝藻属的种类为主,三级河流的优势种主要以菱形藻属的种类为主。随着河流等级增加,曲壳藻科相 对丰度降低,而菱形藻科的相对丰度逐渐升高。冗余分析（RDA）表明,环境选择和空间要素对流溪河硅藻群落结构 均有显著影响,但营养盐水平比空间要素有更高的解释率。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.     

Cardiac,ventilatory and metabolic responses of two ecologically dissimilar species of fish to waterborne cyanide

Changes in heart rate, ventilatory activity and oxygen consumption were determined in trout (Salmo gairdneri) and brown bullhead catfish (Ictalurus nebulosus) during exposure to a steadily increasing concentration of waterborne cyanide selected to produce death in 8–9 hours for each species. The lethal cyanide concentration for the bullheads was an order of magnitude higher than for trout. Trout developed an immediate and gradually increasing bradycardia throughout the exposure period. Cyanide produced tachycardia in the bullhead followed by a gradual onset of bradycardia as the concentration of cyanide was raised. Pericardial injection of atropine (a muscarinic cholinergic antagonist) indicated that bradycardia in the trout was due initially to increased vagal tone but later due to the direct effect of cyanide on the heart. Hyperventilation in the trout persisted throughout the exposure period, although the rate and amplitude fluctuated and was variable between individual fish. During the last hour of exposure (highest cyanide concentration), ventilation was characterized by rapid, shallow breaths followed by a sudden respiratory arrest. The bullheads exhibited hyperventilation during the first 3 hours of exposure followed by a gradual, linear drop in ventilation rate and amplitude until death occurred. Cardiac and ventilatory responses in both species were attributed to stimulation of central and peripheral chemoreceptors by cyanide. Evidence is presented which suggests the initial response in the bullheads was due, at least in part, to gustatory stimulation by the cyanide. Oxygen consumption of the trout remained above pre-exposure levels for the majority of the test period. Oxygen consumption in the bullhead paralleled the changes in heart and ventilatory rates. Whole-body lactate levels of fingerlings of both species during cyanide exposure were measured to estimate the extent of anaerobiosis. Whole-body lactate levels were much greater in the bullheads than the trout, indicating a higher capacity for anaerobiosis, possibly due to a greater fuel supply. Overall, the trout responded to cyanide in a manner similar to that produced by environmental hypoxia whereas the bullheads experienced a gustatory stimulus which masked the hypoxia-like response.     

An overview of stress physiology of fish transport: changes in water quality as a function of transport duration

This study brings an integrated analysis about the relationship between water deterioration and its physiological consequences in live fish transport. The analysis was focused on the transport water and its deterioration, and physiological challenges imposed on the fish. Usual commercial handling procedures employed to mitigate fish stress during transport were discussed. Future topics of research for the establishment of safer fish transport protocols were proposed. Transport was classified into short (≤8 h) or long transport (>8 h). The main issue in short transports should be the prevention of water pH reduction, while in long transports it is the increase in ammonia. Plasma cortisol is the most employed marker for stress and is acutely elevated upon short episodes of transport, but remains elevated even in long‐transport events. Plasma glucose is perhaps a better marker for handling stress. Plasma lactate, pH, osmolality CO₂ and ions should be more often evaluated. Plasma Na⁺ and Cl^‐ are very useful markers of acidosis, due to their respective exchange for H⁺ and , for acid–base regulation. The establishment of species‐specific transport protocols should be preceded by such combined analyses of water and physiological parameters.     

Are hatchery‐reared abalone naïve of predators? Comparing the behaviours of wild and hatchery‐reared northern abalone,Haliotis kamtschatkana (Jonas, 1845)

Abalone populations have declined worldwide, generating interest in enhancement using hatchery‐reared individuals. In many cases, such restoration efforts have met with limited success due to high predator‐induced mortality rates. Furthermore, the mortality rates of outplanted hatchery abalone are often considerably higher than for wild individuals. This study uses northern abalone (Haliotis kamtschatkana) as a case study to determine whether hatchery‐reared abalone behave differently than their wild counterparts. In the field, outplanted hatchery‐reared abalone were significantly less responsive than wild abalone, in terms of number of abalone responding and intensity of response, to nearby movement and to physical contact with an inert probe. Also, when encountering a cue to which all abalone responded (a seastar predator), hatchery‐reared individuals remained subdued. Anti‐predator behavioural deficits in hatchery‐reared abalone were more pronounced in 4‐year‐old individuals than in 1‐year‐old individuals, suggesting an influence of either age or amount of time spent in the hatchery environment. These behavioural differences are expected to increase the vulnerability of hatchery‐reared abalone to predators, and are likely a major cause of their elevated predator‐induced mortality when outplanted.     

Effects of cadmium on the kinetics of calcium uptake in developing tilapia larvae, Oreochromis mossambicus

The toxic effects of Cd²⁺ on Ca²⁺ influx kinetics in developing tilapia (Oreochromis mossambicus) larvae were evaluated. Addition of 20 µg l^-1 of Cd²⁺ to the environment of 0 and 3 day-old larvae competitively inhibited the Ca²⁺ uptake within 4h resulting in a great increase in K_m values for Ca²⁺ influx (19.3 and 17.4 fold, respectively) as compared with their respective controls. Consequently, the actual Ca²⁺ influx of larvae in solutions of 0.2 mM Ca²⁺ are suppressed by 32–45%. Also, 3 day-old larvae were more sensitive to internally accumulated Cd²⁺ than 0 day-old larvae. Although the Ca²⁺ influx in 0 and 3 day-old larvae may be restored to the levels of their respective controls with 24h of being transferred to a 20 µg l^-1 Cd²⁺ solution, total body Ca²⁺ content was significantly reduced in 3 day-old larvae. Increased Ca²⁺ uptake efficiency ensures sufficient Ca²⁺ for normal growth. However, rapid increase in Ca²⁺ influx after hatching also leads to higher Cd²⁺ uptake. Exposure to Cd²⁺ will lead to a drop in body Ca²⁺ content resulting in retardation of larval growth. Therefore, we conclude that if Ca²⁺ uptake is interfered with at this critical stage of development, larvae will not be able to maintain normal levels of body Ca²⁺ and will show signs of Cd²⁺ poisoning.     

Migratory dynamics of stream-spawning longnose gar (Lepisosteus osseus)

Abstract– Literature evidence suggests that lake-dwelling longnose gar (Lepisosteus osseus) enter tributary streams to spawn, Until the present study, the dynamics of this breeding migration had never been investigated quantitatively. During the summers of 1991 and 1992, longnose gar were captured as they entered Weaubleau Creek, Missouri, a tributary of Harry S. Truman Reservoir. The in-stream spawning migration began in early April and ended in late May, and was positively correlated with stream flow and water level, and negatively correlated with water temperature. In-stream residence times ranged from 15 to 94 days, with males exhibiting longer residence times than females. Once in-stream, longnose gar travelled as far as 10 km upstream and occupied certain pools at greater relative frequencies. Although the reason for this preferential utilization is not completely understood, it may relate to pool depth and riffle proximity. Longnose gar disperse from the spawning stream great distances, with gar captured in Weaubleau Creek being recaptured up to 48 km away. This information should provide fisheries biologists the means to consider the reproductive ecology of this species in their conservation and management decisions.     

Nutritional regulation of hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition in zebrafish (Danio rerio) and tilapia (Oreochromis niloticus)

The desaturation and elongation of [1-¹⁴C]18:3n-3 was investigated in hepatocytes of the tropical warm freshwater species, zebrafish (Danio rerio) and Nile tilapia (Oreochromis niloticus). The hepatocyte fatty acid desaturation/elongation pathway was assayed before and after the fish were fed two experimental diets, a control diet containing fish oil (FO) and a diet containing vegetable oil (VO; a blend of olive, linseed and high oleic acid sunflower oils) for 10 weeks. The VO diet was formulated to provide 1% each of 18:2n-6 and 18:3n-3, and so satisfy the possible EFA requirements of zebrafish and tilapia. At the end of the dietary trial, the lipid and fatty acid composition was determined in whole zebrafish, and liver, white muscle and brain of tilapia. Both zebrafish and tilapia expressed a hepatocyte fatty acid desaturation/elongation pattern consistent with them being freshwater and planktonivorous fish. The data also showed that hepatic fatty acid desaturation/elongation was nutritionally regulated with the activities being higher in fish fed the VO diet compared to fish fed the FO diet. In zebrafish, the main effect of the VO diet was increased fatty acid Δ6 desaturase activity resulting in the production of significantly more 18:4n-3 compared to fish fed the FO diet. In tilapia, all activities in the pathway were greater in fish fed the VO diet resulting in increased amounts of all fatty acids in the pathway, but primarily eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3). However, the fatty acid compositional data indicated that despite increased activity, desaturation of 18:3n-3 was insufficient to maintain tissue proportions of EPA and DHA in fish fed the VO diet at the same level as in fish fed the FO diet. Practically, these results indicate that manipulation of tilapia diets in commercial culture in response to the declining global fish oil market would have important consequences for fish fatty acid composition and the health of consumers. Scientifically, zebrafish and tilapia, both the subject of active genome mapping projects, could be useful models for studies of lipid and fatty acid metabolism at a molecular biological and genetic level. This revised version was published online in August 2006 with corrections to the Cover Date.     

Protein and amino acid composition from the mantle of juvenile O ctopus vulgaris exposed to prolonged starvation

Protein and amino acid composition of the mantle of juvenile O ctopus vulgaris (Cuvier, 1797) during fasting for 27 days were determined. Average protein content of octopus mantle was of 711.19 ± 46.80 g kg^?1 DW, and it decreased with increasing fasting days. The non‐essential amino acids content was higher (486.18 ± 11.08 g kg^?1 protein) than essential amino acids (425.82 ± 9.15 g kg^?1 protein) at the start of the experiment (unstarved animals). The results suggest that the amino acid profile of the mantle where the most abundant amino acids are Arg, His, Lys, Gly, Leu and Pro could indicate a prolonged fasting condition (>20 days) or poor nutrition of O . vulgaris. This study supports the idea of using mantle for metabolic needs of starved O . vulgaris suggesting that the degradation pathway of amino acids to pyruvate and tricarboxylic acid cycle intermediates was favoured contrary to the degradation pathway of ketogenic amino acids. Special considerations should be taken concerning Thr, Ile, Ser, Ala, Asx (Asp, Asn), Glx (Glu, Gln) (because of their fast intake) and Lys and His (due to their stable contents) during a prolonged period of fasting.