丁溃疡病的病原菌分离、鉴定与人工感染试验

采用细菌常规分离方法分离丁溃疡病病原,并对所获得的纯培养物进行VITEK-32鉴定和人工感染试验,结果显示:维罗纳气单胞菌温和其变种是丁溃疡病的主要病原菌。     

丁(鱼岁)溃疡病的病原菌分离、鉴定与人工感染试验

采用细菌常规分离方法分离丁(鱼岁)溃疡病病原,并对所获得的纯培养物进行VITEK-32鉴定和人工感染试验,结果显示:维罗纳气单胞菌温和其变种是丁(鱼岁)溃疡病的主要病原菌.     

丁鱥生物学特性及其养殖技术

丁鱥（Tincatmca）隶属于鲤科、雅罗鱼亚科、丁鱥属，广泛分布于欧洲内陆河流、湖泊，在我国分布于新疆。丁鱥是近年来新兴的名优养殖鱼类，因其肉质好而深受消费者欢迎，市场前景看好。近两年，黑龙江省也开始丁鱥养殖试验，养殖效果良好。     

额尔齐斯河经济鱼类——丁鱥

丁鱥亦名须鱥，主要分布于欧洲，目前一些书刊上介绍的欧洲丁桂鱼同我国仅分布于新疆额尔齐斯河和乌伦古河流域的丁鱥是同一条鱼。16-18世纪欧洲就有了池塘养殖丁鱥的记载。但是，该鱼的大规模驯化和人工繁殖近年才开始。丁鱥营养丰富，富含脑黄金(多聚不饱和脂肪酸)，有较好的医用价值。丁鱥肉质细嫩，肉味特别鲜美，其烹饪后体表的胶质尤受人们喜爱，     

丁鱥鱼种水泥池培育试验

丁鱥(欧洲丁鱥)肉味鲜美，具有环境适应范围广、生存温度0～40℃、抗病力强、食性杂、养殖技术简单等优良特点，在国内形成了丁鱥养殖热。为了进一步探索了解苗种生长情况，我们于2003年5月，在水泥池进行了丁鱥苗种培育试验，现将培育试验总结如下：     

周根林套养丁鱥收益好

我们于2004～2005年开展了池塘套养丁鱥技术试验，项目实施两年来取得了较好的成效。2005年在822亩池塘内套养丁鱥，取得了半均亩产丁鱥15．56千克、平均每亩净增收249元的好收成，吴中区横泾镇周根林就是其中的典型，现将其主要技术总结如下：     

寒冷地区丁人工早繁的试验报告

丁鱥（Tinca tinca）属鲤科、雅罗鱼亚科、丁鱥属，本世纪初引入国内池塘养殖，以其优良的经济性状，已经在全世界大面积推广。从2004年起，道外区民主乡一渔场连续引入南方丁鱥夏花培育鱼种并规模化养殖食用鱼，2006年人工繁殖及发塘育苗成功，现在，年产丁鱥食用鱼已达5万kg以上。为了寒冷地区当年育成大规格鱼种，进而实现优质苗种供应本地化的需要，2007年开展了简易节能日光温室产前升温早繁的试验，并获得较好的结果。     

丁鱥鱼种养殖试验

丁鱥（Tican tican）隶属于鲤科、雅罗鱼亚科、丁鱥属。该鱼为底栖杂食性鱼类，在天然水域中以腐败植物残渣及水生昆虫幼虫为食。喜在静水泥底地区生活，冬季耐寒能力强，近几年被我国南方许多城市移植于池塘中饲养，目前主要有四种颜色，即蓝、绿、黄、白，极具观赏价值，易于上钩，是极好的垂钓品种，目前养殖较多的为黄丁鱥与绿丁鱥。     

丁人工繁殖与养殖技术

丁鱥，学名Tinca tinca（Linnaeus），隶属于鲤科、雅罗鱼亚科、丁鱥属，广泛分布于欧洲各地，在欧洲有皇家宠鱼之称，在我国仅分布于新疆额尔齐斯河流域。该品种为淡水底栖鱼类，杂食性，肉质鲜美，生长速度快，对含氧量变化适应性强，经驯化能较好地摄食人工饵料，易于养殖。16—18世纪欧洲就有池塘养殖丁鱥的记载，由于丁鱥的地理分布偏远，国内对其研究一直不多。2001-2002年上海水产大学和新疆建设兵团农十师额河特种鱼类繁育场合作，成功地诱导了丁鱥产卵，目前，福建、广东、湖北、浙江等省市已先后引进试养。现将其人工繁殖及养殖技术介绍如下以供参考。     

丁鱥打印病的防治

丁鱥又称丁桂，隶属于鲤科，雅罗鱼亚科，丁鱥属，广泛分布于欧洲内陆，河流，湖泊，我国分布于新疆的布尔津地区，水温0-40℃下均可存活，生长适温18-30℃。     

新品种条斑紫菜的养殖技术

研究了新品种条斑紫菜的苗种培育、苗网解网、干燥、包装、入库等技术以及紫菜栽培海区的选择和栽培设施等技术关键,引用日本条斑紫菜育苗的技术工艺,采用高密度投放果孢子、丝状体恒温培育、回转式(水车式)采苗等技术,有效控制了出苗帘数量,缩短了育苗时间,提高了壳孢子的利用率,其综合技术经济指标达国内领先水平。     

泥蚶人工育苗及滩涂养成技术研究

通过对泥蚶人工育苗及养成技术生产试验，从饵料、水质、幼虫培育、附着变态、技术、管理措施等方面进行了探讨，取得泥蚶人工育苗生产性突破。共繁育泥蚶苗40．7亿粒，填补了泥蚶工厂化育苗省内空白。     

Immunomodulatory potential of marine secondary metabolites against bacterial diseases of shrimp

Shrimp disease management using bioactive marine secondary metabolites (MSMs) was developed as a package of practice for the sustainable shrimp farming. Therefore, the effect of MSMs on the host defense factors of shrimp was evaluated in the present study. Findings indicated that Ulva diet significantly increase the defense factors such as haemogram, agglutination index, phagocytic rate, bacterial clearance and serum bactericidal activity of treated shrimps over the control group. Based on the gut bacterial load, Ulva diet was considered as proactive drug whereas Dendrilla diet was determined as a curative agent.     

STUDIESONTHEOPTIMALREQUIREMENTSOFPANTOTHENICACID．BIOTIN．FOLICACIDANDB＿（12）INTHESHRIMPPENAEUSCHINENSI

ＳＴＵＤＩＥＳＯＮＴＨＥＯＰＴＩＭＡＬＲＥＱＵＩＲＥＭＥＮＴＳＯＦＰＡＮＴＯＴＨＥＮＩＣＡＣＩＤ．ＢＩＯＴＩＮ．ＦＯＬＩＣＡＣＩＤＡＮＤＢ＿（１２）ＩＮＴＨＥＳＨＲＩＭＰＰＥＮＡＥＵＳＣＨＩＮＥＮＳＩＳＬｉｕＴｉｅｂｉｎ，ＺｈａｎｇＪｉａｍｅｎｇ，Ｌ...     

春季三亚湾浮游动物生态特征与环境因子的关系

采用2011年3月27日-4月3日调查资料,对三亚湾海域浮游动物的种类组成、优势种、生物量、密度、多样性指数和均匀度等分布特征进行分析。结果表明,本次调查鉴定浮游动物68种,优势种为桡足类的瘦歪水蚤（ Tortanus gracilis）、太平洋纺锤水蚤（ Acartia pacifica）、美丽大眼剑水蚤（ Corycaeus speciosus）、小哲水蚤（ Nannocalanus minor）,微刺哲水蚤（Canthocalanus pauper）,锥形宽水蚤（Temora turbinata）;毛颚类的百陶箭虫（Sagitta bedoti）和肥胖箭虫（Sagitta enflata）;介形类的针刺真浮萤（Euconchoecia aculeata）;被囊类的异体住囊虫（ Oikopleura dioica）;樱虾类的中型莹虾（ Lucifer intermedius）。生态类群主要以暖水沿岸种类为主。三亚湾浮游动物密度和生物量分布呈现由河口沿着三亚湾向外海再到鹿回头沿岸呈逆时针逐渐减少趋势,且湾内大于湾外;三亚湾浮游动物的密度与重金属铜呈极显著正相关,与水温呈显著正相关,与活性磷酸盐呈极显著负相关,与盐度呈显著负相关;浮游动物生物量与悬浮物、叶绿素a、铜呈显著正相关,与盐度呈显著负相关。     

Quality improvement by vacuum treatment of boiled skipjack loins for the production of dried skipjack

ABSTRACT: This study attempted to reduce the lipid and water contents in boiled skipjack loins by vacuum treatment and the quality of vacuumed boiled meat was then evaluated during the chilled and frozen storage. The boiled meats were vacuumed at a pressure of 400 Pa for 30 min using a vacuum freeze dryer as an experimental convenience in order to degrease, cool and dehydrate rapidly. The vacuumed boiled meats were stored at 0.0 ± 0.2°C for 10 days and at −20 ± 0.5°C for 90 days. The unvacuumed boiled meats were cooled by air-cooling and stored as the control. Lipid content on a wet basis (w.b.) was reduced as much as 0.5% in the ventral part and 0.4% in the dorsal part of the boiled meats after vacuum treatment. Water content (w.b.) was reduced as much as 2.5% in the ventral part and 3.0% in the dorsal part. The thiobarbituric acid and volatile basic nitrogen values in vacuumed boiled meats were significantly lower ( P < 0.05) than that in unvacuumed boiled meats during storage. Inosine monophosphate (IMP) was only detected as a major nucleotide (about 70%) in boiled meats, and the decomposition of IMP in vacuumed boiled meats was smaller than that in unvacuumed boiled meats during storage. It was concluded that the quality of boiled skipjack loins could be improved by vacuum treatment as a preprocessing procedure in the production of dried skipjack such as katsuobushi .     

长江口最大浑浊带浮游植物的生态研究↑（*）

研究海区浮游植物数量的时空变化具有相当明显的潮汐节律特殊。具体表现为：１、洪、枯水期均为大潮的数量高于小潮的数量，洪季前者为后者的６．８倍；估季前者为后者的６．１倍。２、洪、估期的大小潮期，基本上都以涨憩的数量高于落憩的数量。３、洪、枯季均为大潮期的数量空间分布不均，疏密分布现象明显。     

Identification of pathogenic bacteria associated with the occurrence of 'red spot' in sea mullet, Mugil cephalus L., in south-eastern Queensland

Abstract. An investigation was undertaken to determine the bacterial species associated with the fish disease known as 'red spot' which affected wild fish stocks in south-eastern Queensland. Outbreaks of the disease in the Noosa River were examined with particular reference to the sea mullet, Mugil cephalus. The bacterial flora of the skin of healthy fish as well as those exhibiting early and advanced lesions was determined. In the lower Noosa River estuary, Vibrio anguillarum was the sole organism associated with very early lesions. It was later shown that V. alginolyticus was also associated with V. anguillarum in early lesions, but was considered to be a secondary invader. Aeromonas hydrophila was isolated from advanced lesions on fish taken in freshwater reaches of the river. This organism was also regarded as a secondary invader.     

中华倒刺鲃迟缓爱德华菌的分离鉴定

从患病中华倒刺鲃(Spinibarbus sinensis Bleeker)肝脏中分离得到1株优势菌,命名为SS01。将分离菌株感染健康中华倒刺鲃,人工试验发现其患病症状与自然发病症状一致,半致死剂量(LD50)为2.88×106CFU。根据分离菌株的形态特征、生理生化特性、16S rRNA序列测定结果及系统发育树分析表明菌株SS01为迟缓爱德华菌(Edwadsiella tarda)。药敏试验表明菌株SS01对氟哌酸、恩诺沙星、氟苯尼考、克拉霉素等9种药物敏感,对青霉素、利福平、强力霉素等13种药物表现出耐药性。