芦荟治疗对子宫内膜癌survivin表达的影响

目的:探讨芦荟治疗对子宫内膜癌中凋亡相关基因surv iv in表达的影响。方法:应用芦荟治疗子宫内膜癌患者35例,采用免疫组化(SP)法检测治疗前后癌组织中surv iv in的表达水平。结果:芦荟治疗前后子宫内膜癌组织surv iv in的阳性表达率及表达强度分别为71.4%、(19.36±4.78)和25.7%、(13.35±2.93),治疗后子宫内膜癌surv iv in阳性表达率及表达强度显著降低(P<0.01)。子宫内膜癌组织中surv iv in表达强度与组织学分级、手术分期、肿瘤大小均呈正相关(r=0.412,0.398,0.408,P均<0.05)。结论:芦荟治疗子宫内膜癌的作用可能与其抑制癌组织surv iv in的表达有关。     

渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究(英文)

[Objective] The study aimed to reveal the effect of osmosis-regulating substances and organic appendices on somatic embryogenesis in wheat. [Method] The suitable concentration combination of appendices was optimized by adding different concentrations of osmosis-regulating substances including mannitol, sorbitol and organic appendices such as Gln, CH and LH, into the somatic embryogenesis in wheat. [Result] The mannitol or sorbitol lower than 40 g/L was helpful for improving somatic embryogenesis; there was no significant difference in the induction rate of somatic embryogenesis when 300-500 mg/L Gln、CH or LH was respectively added into the induced medium, while somatic embryogenesis could be enhanced dramatically in the presence of 500 mg/L Gln together with 300 mg/L CH. [Conclusion] Somatic embryogenesis could be improved to some extent by different concentrations of osmosis-regulating substances and organic appendices, which laid foundation for establishing a more perfect system of somatic embryogenesis in wheat.     

沙枣引种试栽研究(英文)

[Objective] The experiment aimed to explore the possibility of introducing Elaeagnus angustifolia L. in saline land of West Jilin Province for improving local ecological environment. [Method] The Elaeagnus angustifolia L. from Ningxia Province were sown in nursery and practice base of Forestry College in Beihua University to observe their phenophase, growth state, stress resistance and study the relations between survival rate and different cutting medium, grafting methods. [Result] Elaeagnus angustifolia L. could adapt to climate condition in Jilin area and grow well . Among 3 cold-proof measures, there was no freezing injury by soil burial method, although there was 2.8% and 3.9% mortality by covering grassy marshland and winding straw, the cold injury degree was light, so they could go through winter normally. Meanwhile, Elaeagnus angustifolia L. had good saline-alkali tolerance, drought resistance and flood resistance. The successful cutting and graft of Elaeagnus angustifolia L. provided a condition for planting in large-scale and knew the rooting rate of different cutting medium. [Conclusion] Elaeagnus angustifolia L. could be introduced to plant in West Jilin Province.     

杜鹃花色素的提取及稳定性研究(英文)

[Objective] The aim of this study was to further investigate and utilize the natural anthocyanidin from Rhododendron L. [Method] Using Rhododendron L. with four different colors including white, pink, red and purple as the test materials, its anthocyanidin extraction methods were analyzed in this study to evaluate the spectral properties and its stability of various anthocyanidin. [Result] The anthocyanidin from Rhododendron L. was perfectly extracted by methanol with 1% concentrated hydrochloric acid (V/V) and had better stability in this extraction solution. The further experiment in vitro indicated that the anthocyanidin from Rhododendron L. became stable with pH value of 0-3, but could not resistant to high temperature or strong light, and the alkaline condition had also great effects on its stability. [Conclusion] The methanol with 1% concentrated hydrochloric acid (V/V) has the best effect for extraction, and the anthocyanidin from Rhododendron L. is more stable in low temperature, weak light and acid conditions.     

流加培养方式对大肠杆菌XD-12发酵的影响研究

[目的]研究大肠杆菌流加培养方式,提高转氨酶供体——大肠杆菌XD-12的发酵浓度。[方法]通过研究碳源流加、氮源流加、pH控制流加对发酵的影响,获得了优化的培养条件。[结果]产转氨酶大肠杆菌的最佳培养条件为：温度37℃,搅拌转速500 r/m in,通气量1.5 L/m in,培养基初始pH为7.0,控制发酵过程pH为7.5,初始葡萄糖浓度5 g/L,初始氮源为5 g/L蛋白胨＋1.5 g/L牛肉膏,从葡萄糖浓度下降为2 g/L开始每隔2 h间歇流加120 g/L的糖,从8 h起每隔2 h间歇流加15 g/L蛋白胨＋4.5 g/L牛肉膏。在此条件下培养24 h,大肠杆菌的菌体干重浓度达9.66 g/L,较分批培养提高了104.7%。[结论]这对降低酶法制备L-苯丙氨酸的生产成本、提高生产效率、满足日益增长的市场需求具有重要的现实意义。     

Effect of Phytohormones on Adventitious Bud Differentiation from Bulb Scales of Oriental Lily Test-tube Plantlets

[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS＋0.2 mg/L 6-BA＋0.2 mg/L 2,4-D, MS＋I.0 mg/L 6-BA＋0.2 mg/L IAA, MS＋ 1.0 mg/L KT＋0.5 mg/L 2,4-D, respectively.     

泥胡菜的组织培养及高效无性系建立(英文)

[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in calluses induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied. [Result] The results showed that the optimum medium for granulated calluses induction from tender stem was MS+BA 0.3 mg/L+2,4-D 1-1.5 mg/L, for granulated calluses and adventitious bud differentiation was MS+AgNO3 1.5 mg/L +BA 0.4 mg/L +NAA 0.1 mg/L. 1/2 MS+IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration, and cinder was used as transplantation and cutting substrate. [Conclusion] This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.     

龙柏组织培养研究

[目的]为龙柏的快速繁殖提供参考。[方法]用圆柏（对照材料）、龙柏的当年生幼嫩茎段为外植体,以MS为基本培养基附加不同浓度的激素对其进行诱导分化和增殖研究,筛选诱导芽分化、增殖的最佳激素组合。[结果]接种前,将圆柏和龙柏的外植体用浓度75%乙醇浸泡30 m in,无菌水冲洗几次,用浓度0.2%HgC l2消毒5 m in,无菌水洗5次,然后置于培养皿上沥干,消毒效果最好。在1/2MS＋6-BA 0.5 mg/L＋NAA 0.5 mg/L的培养基上,圆柏试管苗分化率最高,生长良好;在1/2MS＋6-BA 1.5 mg/L＋NAA 0.5 mg/L的培养基上,龙柏试管苗分化率最高,生长良好。在MS＋6-BA 1.0 mg/L＋NAA 0.01 mg/L的培养基上,龙柏增殖效果最好。[结论]建立了龙柏组织培养体系。     

微波催陈黄酒的研究

[目的]为人工催陈黄酒提供试验依据。[方法]以新酿制黄酒为研究对象,在单因素试验的基础上,通过正交试验对微波催陈黄酒的工艺进行研究。[结果]微波催陈黄酒的较佳工艺条件为：微波处理能量87.5 J/s、微波处理时间3 m in、柠檬酸添加量0.3%（M/M）、微波作用次数为1次。该条件下催陈黄酒中还原糖含量为24.07 g/L、总酸含量为5.12 g/L、总酯含量为3.89 g/L、pH为4.18,总酸、总酯和风味物质组成与三年自然陈酿相近。[结论]采用微波技术催陈黄酒,风味物质显著提高,达到了人工催陈的效果。     

土壤镉污染对大蒜生理生化指标的影响(英文)

[Objective] The experiment aimed to study the effects of cadmium pollution in soil on physiological and biochemical index of Allium sativum L. and provided reference for the recovery of cadmium pollution in soil. [Method]By setting eleven Cd concentrations from 0.21 to 500 mg/kg in soil and the pot test, ecological corresponding mechanism of plant height, chlorophyll (Chl) content, catalase (CAT) activity and malondialdehyde (MDA) of Allium sativum L. was analyzed. [Result] The plant height had a strong tolerance to cadmium pollution in soil, while the total chlorophyll content and chlorophyll a content had no significant difference compared with control treatment, except Cd concentration was 500 mg/kg. The high Cd concentration would increase the damage to membrane of Allium sativum L. however with the regulation of physiological mechanism, the damage was gradually decreased.[Conclusion] Allium sativum L. had strong eco-physiological adaptability to Cd contaminated soil and it had potential for recovering Cd contaminated soil.     

生长素介导下三唑醇在大豆植株内的传导性(英文)

[Objective] This study was to investigate the mobility of IAA-triadimenol and NAA-triadimenol in soybean seedlings. [Method] Soybean seedlings were treated by spraying the solution of conjugated compounds onto their leaves; and chromatography was employed to measure the contents of IAA-triadimenol and NAA-triadimenol in different parts of these soybean seedlings, through which to represent the mobility of the conjugates. [Result] Both the triadimenol and NAA-triadimenol could not transport basipetally; whereas IAA-triademenol was ambimobile. When sprayed with 0.5 mmol/L IAA-triadimenol, as much as 1.87 μg/(g·FW) IAA-triadimenol was detected in the roots of soybean seedlings 12 h later, which was higher than that in the stem [0.68 μg/(g·FW)]; while that decreased to 0.80 μg/(g·FW) in the seedlings sprayed with 0.5 mmol/L IAA-triadimenol and the same concentration of IAA. [Conclusion] IAA moiety of the conjugate could enhance the transport and accumulation of the fungicide towards the root via the IAA carriers.     

不同培养基和泡水时间处理对油茶花粉萌发率的影响

[目的]研究不同处理方式对油茶（Camellia oleifera）花粉萌发率的影响。[方法]油茶品种为望漠油茶和岑溪软枝油茶。共设不同泡水时间和培养基处理。其中,泡水时间分别为0、10、20、30 m in,培养基分别为5%蔗糖＋100 mg/L硼酸＋3%琼脂、10%蔗糖＋100mg/L硼酸＋3%琼脂和20%蔗糖＋100 mg/L硼酸＋3%琼脂。[结果]在不泡水的情况下,望漠油茶花粉萌发最佳培养基是5%蔗糖＋100 mg/L硼酸＋3%琼脂,其花粉的萌发率为80.4%;岑溪软枝油茶花粉萌发的最佳培养基是10%蔗糖＋100 mg/L硼酸＋3%琼脂,其花粉的萌发率为88.5%。在采用相同培养基时,对油茶花粉泡水10~30 m in,花粉的平均萌发率仅为6.3%,最高萌发率为17.9%。[结论]该研究说明泡水对油茶花粉的生命力有极大损害。因此,在油茶人工辅助授粉中不宜采用水粉喷雾法。     

雌二醇对体外培养绒山羊初级毛囊的影响(英文)

[Objective] The aim of this study was to preliminarily explore the effects of estradiol on morphology and growth of cashmere goat primary hair follicles. [Method] Cashmere goat primary hair follicles were cultured in serum-free Williams E media supplemented with different doses of 17 β-E2 (0, 0.1, 1.0, 10.0, 100.0 nmol/L), and their growth rates and morphological changes were observed. [Result] The growth rate of 0.1 nmol/L 17 β-E2 group was quite comparable with that of the control group(0 nmol/L), but the 17 β-E2 with concentrations of 1.0, 10.0 and 100.0 nmol/L displayed different degrees of inhibition on the growth of hair follicles. Different morphological changes of hair follicles could also be discovered in different concentration treatments. [Conclusion] The study laid a certain foundation for exploring the regulation mechanism of estrogen on growth of cashmere goat hair follicles.     

NaCl胁迫对普陀樟叶绿素荧光参数的影响(英文)

[Objective] Study on the changes of chlorophyll fluorescence parameters in Cinnamomum japonicum var. chenii under NaCl stress. [Method] The seedling growth increment,chlorophyll content and chlorophyll fluorescence parameters in leaves of 1-year old Cinnamomum japonicum var. chenii were investigated in field experiment. [Result] Under NaCl stress,seedling growth increment reduced and the chlorophyll content decreased to a stable value; changes of Fv/Fm and Fv/Fo showed identical increasing trend and double peak type. With the aggravation of salt stress,most variations were observed in Fo,correlations among chlorophyll fluorescence parameters presented "rise-drop" trend (in the treatment of 7 g/L NaCl). [Conclusion] Cinnamomum japonicum var. chenii is endowed with strong salt resistance and wide adaptability.     

Effects of NaCl Stress on Chlorophyll Fluorescence Parameters in Cinnamomum japonicum var. chenii

[Objective] Study on the changes of chlorophyll fluorescence parameters in Cinnamomum japonicum var. chenii under NaCl stress. [Method] The seedling growth increment,chlorophyll content and chlorophyll fluorescence parameters in leaves of 1-year old Cinnamomum japonicum var. chenii were investigated in field experiment. [Result] Under NaCl stress,seedling growth increment reduced and the chlorophyll content decreased to a stable value; changes of Fv/Fm and Fv/Fo showed identical increasing trend and double peak type. With the aggravation of salt stress,most variations were observed in Fo,correlations among chlorophyll fluorescence parameters presented "rise-drop" trend (in the treatment of 7 g/L NaCl). [Conclusion] Cinnamomum japonicum var. chenii is endowed with strong salt resistance and wide adaptability.     

阴香扦插繁殖试验

[目的]探讨基质和生根剂对阴香扦插生根的影响。[方法]采用田间扦插试验研究不同基质、不同种类和不同浓度的生根促进剂对阴香扦插生根的影响。[结果]阴香扦插的基质以黄心土和珍珠岩为好。200 mg/L ABT+黄心土+15 m in和100 mg/L ABT+黄心土+30 m in组合的生根率最高。基质对生根率影响较大,基质间不同浓度的生根剂对生根率影响显著,而生根剂对生根率的影响不显著,生根剂之间不存在差异。生根进程中POD和CAT活性随插条生根的进程逐渐增加,有利于难生根的植物插条的扦插生根。[结论]采用当年生半木质化或1年生木质化程度不高的插条,以黄心土或珍珠岩为基质,选用ABT为生根剂可提高阴香生根率。     

复合诱变选育丙酮丁醇梭菌发酵玉米秸秆水解液

[目的]提高丙酮丁醇梭菌CICC8012产丁醇能力。[方法]采用紫外线和甲基磺酸乙酯（EMS）复合诱变选育丙酮丁醇梭菌CICC8012。[结果]紫外辐照120 s,5%EMS处理60 m in条件下,筛选得到1株遗传稳定性良好的高产突变株M-31,其在葡萄糖培养基中总溶剂产量10.39 g/L,丁醇产量6.55 g/L,较出发菌株分别提高了16.48%和20.62%。对M-31玉米秸秆水解液发酵培养基进行优化,得到最优工艺组合：初始水解糖浓度为80 g/L,（NH4）2SO43 g/L,KH2PO40.6 g/L,MgSO4.7H2O 0.4 g/L,FeSO4.7H2O 15 mg/L,并选择亚硫酸盐法对秸秆水解液进行脱毒,丁醇和总溶剂产量分别达到5.19和8.27 g/L,较优化前分别提高了55.39%和55.74%。[结论]得到的高产突变株较出发菌株丁醇产量更高、更适合于生物质发酵。     

丁香酚对中国对虾幼虾麻醉效果的初步研究(英文)

[Objective] The aim of the study is to seek a good anesthetic to Fenneropenaeus chinensis. [Method] The anesthetic effect of eugenol to juvenile Fenneropenaeus chinensis was investigated. [Result] The juveniles could be effectively anaesthetized by 50-400 mg/L eugenol aqueous solution with temperature of 24 ℃. Within the concentration range of 50-400 mg/L, the increase of the eugenol concentration could shorten the time required for anesthesia, meanwhile could prolong the time for recovery. The recovered rate of prawn reached 100% when the eugenol concentration was lower than 200 mg/L, while the recovered rate of prawn was just 66.67% when the eugenol concentration was higher than 400 mg/L. The survival rate of prawns in test group was 100% from the observation of three consecutive days. For the specific dose, the anesthetic effect enhanced with the increase of water temperature (18-27℃). [Conclusion] Eugenol is a safe and efficient anesthetics that can be applied in genetic breeding of prawn.     

百蕊草无性系建立与瓶外生根研究(英文)

[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.[Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were designed to conduct induction culture,strengthening plantlet culture and in vitro rooting.[Result]The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA,0.01 mg/L NAA and 0.3 mg/L 2,4-D;in addition,60 mg/kg ABT was suitable for rooting,by which the percentage of rooted plantlets reached 76.6%.[Conclusion]This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate,providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.     

长寿花快繁及试管苗开花研究

[目的]探讨长寿花试管苗培养及诱导开花的关键技术。[方法]以长寿花带芽茎段为材料,研究HgC l2消毒时间、植物生长调节剂配比对外植体污染率、增殖效果及试管苗开花的影响。[结果]HgC l2消毒8 m in效果最好,污染率为20%,明显低于不消毒的77.77%;培养基中6-BA添加量为2.8 mg/L时,试管苗明显高于添加量为1.0、1.5、2.1 mg/L的处理,IAA对丛芽增殖影响不明显,但添加量过高抑制丛芽增殖。增殖培养基中植物生长激素的最佳组合为：0.30 mg/L IAA＋2.1 mg/L 6-BA＋0.6 mg/L GA3;NAA添加量为2.0-4.5 mg/L时,均能诱导花芽分化,诱导率为5.5%-35.6%,单独使用GA3不能诱导试管苗开花,但GA3与NAA配合使用可使试管苗提前15 d开花。[结论]诱导长寿花试管苗增殖及开花的最佳培养基分别为MS＋0.30 mg/L IAA＋2.1 mg/L 6-BA＋0.6 mg/LGA3和MS＋2.5 mg/L NAA。