| 百蕊草无性系建立与瓶外生根研究(英文) |
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| 引用本文： | 江芹,董玲,宁志怨,陈静娴,廖华俊.百蕊草无性系建立与瓶外生根研究(英文)[J].农业科学与技术,2008,9(5):47-49. |
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| 作者姓名： | 江芹 董玲 宁志怨 陈静娴 廖华俊 |
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| 作者单位： | 安徽省农业科学院园艺研究所 |
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| 基金项目： | 安徽省农业科学院重点及新兴学科培育项目
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| 摘 要： | Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were designed to conduct induction culture,strengthening plantlet culture and in vitro rooting.Result]The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA,0.01 mg/L NAA and 0.3 mg/L 2,4-D;in addition,60 mg/kg ABT was suitable for rooting,by which the percentage of rooted plantlets reached 76.6%.Conclusion]This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate,providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.
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| 关 键 词： | Thesium chinense Turcz Clustered shoots Rooting |
Establishment of Somatic Cell Clones in Thesium chinense Turcz and Its in vitro Rootinq Technique |
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| JIANG Qin,DONG Ling,NING Zhi-yuan,CHEN Jing-xian,LIAO Hua-jun.Establishment of Somatic Cell Clones in Thesium chinense Turcz and Its in vitro Rootinq Technique[J].Agricultural Science ＆ Technology,2008,9(5):47-49. |
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| Authors: | JIANG Qin DONG Ling NING Zhi-yuan CHEN Jing-xian LIAO Hua-jun |
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| Institution: | The Horticulture Institute of Anhui Academy of Agricultural Sciences,Hefei 230031 |
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| Abstract: | Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz. Method] With stem frag-ments of wild Thesium chinense Turcz as explants, different culture media were designed to conduct induction culture, strengthening plantlet culture and in vitro rooting. Result] The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA, 0. 01 mg/L NAA and 0.3 mg/L 2,4-D; in addition, 60 mg/kg ABT was suitable for rooting, by which the percentage of rooted plantlets reached 76.6%. Conclu-sion] This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate, providing basis for artificial cul-tivation and resource protection of Thesium chinense Turcz. |
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| Keywords: | Thesium chinense Turcz Clustered shoots Rooting |
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