蝴蝶兰‘红天鹅’组织培养及快繁技术

[目的]探索‘红天鹅’花梗的不定芽诱导、丛生芽继代增殖和生根诱导技术。[方法]以蝴蝶兰新品种‘红天鹅’的花梗作为外植体,开展组织培养技术研究。[结果]用0.1%的HgCl2消毒外植体20 min,污染率可降低至27.2%,萌芽率达91.0%;不定芽继代增殖培养的最适培养基为MS+6-BA 6.0 mg/L+NAA 0.1 mg/L+AD 5.0 mg/L+香蕉50 g/L+土豆50g/L+蔗糖30g/L+琼脂7.5 g/L,增殖系数达3.80;最适合的生根诱导培养基为1/2MS+NAA0.4 mg/L+香蕉50 g/L+土豆50g/L+C 1.0 g/L+蔗糖15 g/L+卡拉胶7.0 g/L,生根时间为15 d,生根率达100%。[结论]发现了适宜‘红天鹅’的从消毒到增殖,再到生根中各试剂和激素的合理用量,为蝴蝶兰快繁技术发展提供了参考。     

不同培养基成分对薄荷试管苗生根的影响

[目的]筛选薄荷试管苗生根的最佳培养基组合。[方法]以薄荷试管苗茎段为试验材料,探讨不同培养基成分、凝固剂种类及添加量对其生根的影响。[结果]在薄荷试管苗培养过程中,使用琼脂为凝固剂,且添加量为7 g/L的1/2MS培养基时,其生根时间最短,生根数和生根长度较高。[结论]为薄荷试管苗的大规模、优质、高效生产提供理论依据。     

葡萄组培苗瓶外生根技术研究

采用不同种类和浓度的生长素诱导葡萄组培苗瓶外生根，并观察组培苗在不同处理基质、不同的温度、不同的生理年龄条件下生根的效果。通过优化技术参数，建立了葡萄组培苗瓶接生根的技术优化体系，结果表明，用浓度为0.3mg/L的IBA浸蘸处理的插条，在温度为27~29℃的环境条件下在基质珍珠岩上培养，瓶苗茎段的生根率可达85.5 %，移栽后苗木的成活率达98 %，相对于其他方法有了较大的提高。该技术的建立有助于实现葡萄工厂化育苗技术的商业化扩大。     

驱蚊草组培快繁技术研究初报

以驱蚊草茎段为试材，正交设计法考察3种激素对其增殖率的影响，结果表明，MS+6-BA1.0 mg/L +IBA0.1 mg/L +GA3 1.1 mg/L为增殖培养的适宜培养基。1/2MS+ IBA0.1mg/L为生根培养的适宜培养基。     

Variation in Shoot Anatomy and Rooting Behaviour of Stem Cuttings in Relation to Age of Donor Plants in Teak (Tectona grandis Linn. f.)

Shoot anatomical features viz., number of vessel elements, width and length of vessel and fibre elements were studied in relation to juvenility/maturity of teak (Tectona grandis Linn. f.). The findings show significant differences in these features, which indicates that variation in shoot anatomy can be used as a reliable indicator of juvenility/maturity in teak. The effect of age of donor plants and application of auxins on adventitious root formation in single noded leafy stem cuttings of teak was also investigated. Aging of donor trees suppressed rooting and sprouting of cuttings, but increased callus formation at the base of cuttings. Per cent rooting and per cent sprouting in cuttings decreased as age of donor plants increased from 2-months to 15-years and up to 30-years. IBA treatment promoted per cent rooting as well as per cent sprouting, NAA promoted only rooting and it suppressed sprouting. The effect of auxin treatment on rooting and sprouting of teak stem cuttings varied with the age of donor plants. Auxin and its concentration, which suppressed rooting and sprouting in cuttings of younger donor plants, actually promoted rooting when cuttings were taken from more mature donor plants. It appears that the auxin requirement for causing and promoting rooting in teak cuttings increased with increasing age of donor plants. The higher auxin requirement for causing and promoting rooting in cuttings of older trees may be due to a decrease in the content on endogenous auxins or decreased sensitivity of aging tissues to rooting promoters and /or accumulation of inhibitory substances which inhibit rooting.     

香椿组织培养与快速繁殖的研究

香椿试管苗在不同培养条件下增殖速度明显不同.太行山香椿试管苗的最佳增殖条件为改良MS十SBA1.0十GA_32.0+IAA0.0;燕山香椿试管苗则为WPM+6BA1.0+GA_32.0+IAA0.0。试管苗的生根能力在IBA0.2～1.0的范围内逐渐提高.增殖倍数不同试管苗其K的摄入量、碳水化合物含量以及POD和EST同工酶图谱亦有明显差别。     

Root growth enhancement by rhizospheric aluminum treatment in Quercus serrata Thunb. seedlings

This article discusses the effect of aluminum on root growth of Querucus serrata Thunb. In a 9-week hydroponics experiment, the effects of various concentrations (0 to 5.0 mM) of Al on root growth were examined. Results revealed that root biomass increases with the increase in Al concentration up to 2.5 mM, and then it tended to decrease. In the next experiment, the effects of H⁺ and Al³⁺ on roots were compared in a 4-week hydroponics experiment using three treatment solutions: control (pH 6.0), −Al (without Al, pH 3.5), and +Al (with 2.5 mM Al, pH 3.5). No clear difference in the biomass and root length between the control and −Al treated roots was observed, and root and shoot biomass were increased by Al treatment. These results confirmed that the H⁺ concentration level, at a pH of 3.5, is not toxic for Q. serrata and the Al-induced increase in root biomass is not caused by the amelioration of H⁺ toxicity by Al. In the third experiment, roots were exposed to an Al solution (pH 3.6) intermittently. This treatment clarified that Al stimulated rooting and root elongation. In the fourth experiment, the effect of 1 mM Al on root growth during a 15-month period in a sand culture were examined. This experiment confirmed that Al stimulated good growth and development of root systems at appropriate concentrations. Therefore, it is considered that Al-induced root growth enhancement occurs as a long-term and short-term phenomenon.     

金丝垂柳扦插繁殖试验

金丝垂柳扦插繁育技术的研究结果表明 :金丝垂柳插条主要以皮部生根为主 ;ABT 1号生根粉 10 0 0× 10 -6mg/ ml处理 ,促进插条生根效果最显著 ,插穗长势最好。其它处理效果由强到弱为 ABT 1号生根粉 5 0 0× 10 -6mg/ ml、南林 I型生根粉、 ABD生根剂 10 0 0× 10 -6mg/ ml和清水。     

抗生素对火炬松的器官发生植株再生的影响(英文)

本文研究了氨苄青霉素、羧苄青霉素和头孢霉素等3种抗生素对火炬松愈伤组织的生长和分化及不定芽生根的影响。结果表明,头孢霉素最有利于愈伤组织的诱导和生长,羧苄青霉素最有利于芽的分化,氨苄青霉素降低了不定芽的生根频率。所有试验的3种抗生素提高愈伤组织的形成和芽再生,但降低了芽的生根频率。这些结果表明,选择合适的抗生素对优化火炬松遗传转化体系有重要作用。图3表4参25。     

Erythrina brucei: Propagation attributes, leaf nutrient concentration and impact on barley grain yield

Erythrina brucei Schweinf. emend. Gillett (Papilionaceae),a tree species that is endemic to Ethiopia, is known to possess useful agroforestry attributes, but these attributes have not been quantified. To achieve this, propagation of the species, growth rate of young trees and their impact on barley (Hordeum vulgare L.), and leaf-nutrient concentrations were examined. Germination of seeds planted in plastic pots varied from 73 to 93%, and direct seeding in the field yielded 83%germination. Branch cuttings obtained from 2 to 3-year-old stock plants (n =150) resulted in 70 to 80% rooting within 8 to 12 weeks after setting cuttings into the rooting medium. Considerable nodulation occurred on leaves one year after planting out seedlings and stecklings. Leaf concentrations of Mg, N,P, and S were significantly lower in nodulated than in the non-nodulated leaves. On the other hand, leaf Fe concentration was higher in nodulated than in the non-nodulated leaves. There was no significant difference in Ca and K contentsbetween the nodulated and non-nodulated leaves. E. brucei-treatment significantly increased tiller and seed numbers, as well as seed weight. However, thousand-grain weight and grain size were similar in harvests from E. brucei-treated and the control plots. The results indicate that the species is easy to propagate by seed and vegetative means, grows fast, is desirable for intercropping, and has relatively high leaf-nutrient concentration.This revised version was published online in November 2005 with corrections to the Cover Date.